EGTA AM *CAS 99590-86-0*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
Quotation | Request |
International | See distributors |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 668.6 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C) |
UNSPSC | 12352200 |
Alternative formats
EGTA AM *10 mM DMSO solution* |
Overview | ![]() ![]() |
CAS 99590-86-0 | Molecular weight 668.6 |
EGTA AM is cell-permeable version of EGTA (ethylene glycol tetraacetic acid), a cell-permeable calcium chelator. This EGTA derivative is used for adjusting calcium concentrations in cells and tissues. EGTA is an aminopolycarboxylic acid, a chelating agent. Compared to EDTA, EGTA has a lower affinity for magnesium, making it more selective for calcium ions. It is useful in buffer solutions that resemble the environment in living cells where calcium ions are usually at least a thousand fold less concentrated than magnesium. The pKa for binding of calcium ions by tetrabasic EGTA is 11.00, but the protonated forms do not significantly contribute to binding, so at pH 7, the apparent pKa becomes 6.91.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of EGTA AM *CAS 99590-86-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 149.566 µL | 747.831 µL | 1.496 mL | 7.478 mL | 14.957 mL |
5 mM | 29.913 µL | 149.566 µL | 299.133 µL | 1.496 mL | 2.991 mL |
10 mM | 14.957 µL | 74.783 µL | 149.566 µL | 747.831 µL | 1.496 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
Name | Excitation (nm) | Emission (nm) |
BCECF, AM | 504 | 527 |
BCECF, AM *UltraPure grade* | 504 | 527 |
Zinquin AM *UltraPure grade* | 355 | 491 |
Calcein, AM *CAS 148504-34-1* | 501 | 521 |
Calcein, AM *UltraPure grade* *CAS 148504-34-1* | 501 | 521 |
Citations
View all 6 citations: Citation Explorer
Vesicular Glutamate Transporter Expression Ensures High-Fidelity Synaptic Transmission at the Calyx of Held Synapses
Authors: Nakakubo, Yutaro and Abe, Saeka and Yoshida, Tomofumi and Takami, Chihiro and Isa, Masayuki and Wojcik, Sonja M and Brose, Nils and Takamori, Shigeo and Hori, Tetsuya
Journal: Cell Reports (2020): 108040
Authors: Nakakubo, Yutaro and Abe, Saeka and Yoshida, Tomofumi and Takami, Chihiro and Isa, Masayuki and Wojcik, Sonja M and Brose, Nils and Takamori, Shigeo and Hori, Tetsuya
Journal: Cell Reports (2020): 108040
TBC1D9 regulates TBK1 activation through Ca 2+ signaling in selective autophagy
Authors: Nozawa, Takashi and Sano, Shunsuke and Minowa-Nozawa, Atsuko and Toh, Hirotaka and Nakajima, Shintaro and Murase, Kazunori and Aikawa, Chihiro and Nakagawa, Ichiro
Journal: Nature communications (2020): 1--16
Authors: Nozawa, Takashi and Sano, Shunsuke and Minowa-Nozawa, Atsuko and Toh, Hirotaka and Nakajima, Shintaro and Murase, Kazunori and Aikawa, Chihiro and Nakagawa, Ichiro
Journal: Nature communications (2020): 1--16
Distortion of the Actin A-Triad Results in Contractile Disinhibition and Cardiomyopathy
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell Reports (2017): 2612--2625
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell Reports (2017): 2612--2625
Distortion of the actin A-triad results in contractile disinhibition and cardiomyopathy
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell reports (2017): 2612--2625
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell reports (2017): 2612--2625
Routes of Ca2+ Shuttling during Ca2+ Oscillations FOCUS ON THE ROLE OF MITOCHONDRIAL Ca2+ HANDLING AND CYTOSOLIC Ca2+ BUFFERS
Authors: Pecze, László and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230
Authors: Pecze, László and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230
Routes of Ca2+ shuttling during Ca2+ oscillations: Focus on the role of mitochondrial Ca2+ handling and cytosolic Ca2+ buffers
Authors: Pecze, L{\'a}szl{\'o} and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230
Authors: Pecze, L{\'a}szl{\'o} and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230
References
View all 77 references: Citation Explorer
Tuning caged calcium: photolabile analogues of EGTA with improved optical and chelation properties
Authors: Ellis-Davies GC, Barsotti RJ.
Journal: Cell Calcium (2006): 75
Authors: Ellis-Davies GC, Barsotti RJ.
Journal: Cell Calcium (2006): 75
Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG
Authors: Fisher AE, Hague TA, Clarke CL, Naughton DP.
Journal: Biochem Biophys Res Commun (2004): 163
Authors: Fisher AE, Hague TA, Clarke CL, Naughton DP.
Journal: Biochem Biophys Res Commun (2004): 163
Voltage-activated calcium signals in myotubes loaded with high concentrations of EGTA
Authors: Schuhmeier RP, Dietze B, Ursu D, Lehmann-Horn F, Melzer W.
Journal: Biophys J (2003): 1065
Authors: Schuhmeier RP, Dietze B, Ursu D, Lehmann-Horn F, Melzer W.
Journal: Biophys J (2003): 1065
Gravicurvature loss, changes in ultrastructure and calcium balance of pea root statocytes treated with EGTA
Authors: Belyavskaya NA., undefined
Journal: J Gravit Physiol (2001): P33
Authors: Belyavskaya NA., undefined
Journal: J Gravit Physiol (2001): P33
L-type calcium channel blockers and EGTA enhance superoxide production in cardiac fibroblasts
Authors: Shivakumar K, Kumaran C.
Journal: J Mol Cell Cardiol (2001): 373
Authors: Shivakumar K, Kumaran C.
Journal: J Mol Cell Cardiol (2001): 373
Role of calcium in nitric oxide-induced cytotoxicity: EGTA protects mouse oligodendrocytes
Authors: Boullerne AI, Nedelkoska L, Benjamins JA.
Journal: J Neurosci Res (2001): 124
Authors: Boullerne AI, Nedelkoska L, Benjamins JA.
Journal: J Neurosci Res (2001): 124
Correlation of EGTA and calcium-blocking agents on the response of the bladder to in vitro ischemia
Authors: Levin RM, Leggett R, Whitbeck C, Horan P.
Journal: Pharmacology (1999): 113
Authors: Levin RM, Leggett R, Whitbeck C, Horan P.
Journal: Pharmacology (1999): 113
Effect of sarcoplasmic reticulum (SR) calcium content on SR calcium release elicited by small voltage-clamp depolarizations in frog cut skeletal muscle fibers equilibrated with 20 mM EGTA
Authors: Pape PC, Carrier N.
Journal: J Gen Physiol (1998): 161
Authors: Pape PC, Carrier N.
Journal: J Gen Physiol (1998): 161
Effects of partial sarcoplasmic reticulum calcium depletion on calcium release in frog cut muscle fibers equilibrated with 20 mM EGTA
Authors: Pape PC, Jong DS, Ch and ler WK., undefined
Journal: J Gen Physiol (1998): 263
Authors: Pape PC, Jong DS, Ch and ler WK., undefined
Journal: J Gen Physiol (1998): 263
Fura-2 calcium signals in skeletal muscle fibres loaded with high concentrations of EGTA
Authors: Struk A, Szucs G, Kemmer H, Melzer W.
Journal: Cell Calcium (1998): 23
Authors: Struk A, Szucs G, Kemmer H, Melzer W.
Journal: Cell Calcium (1998): 23
Application notes
A Comparison of Fluorescent Red Calcium Indicators for Detecting Intracellular Calcium Mobilization in CHO Cells
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
A Meta-Analysis of Common Calcium Indicators
A New Red Fluorescent & Robust Screen Quest™ Rhod-4™ Ca2+Indicator for Screening GPCR & Ca2+ Channel Targets
A New Robust No-Wash FLIPR Calcium Assay Kit for Screening GPCR and Calcium Channel Targets
A Novel NO Wash Probeniceid-Free Calcium Assay for Functional Analysis of GPCR and Calcium Channel Targets
FAQ
Are there any calcium indicators that don't require probenecid (PBC)?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
How do I make an AM ester stock solution?
Are there upgraded trypan blue derivatives for cell viability testing?
Can I intracellularly measure mitochondria calcium flux and changes in mitochondria membrane potential at the same time?
Do you offer any products for measuring intracellular calcium concentration or movement by flow cytometry?
How do I make an AM ester stock solution?