EGTA AM CAS 99590-86-0

EGTA AM is cell-permeable version of EGTA (ethylene glycol tetraacetic acid), a cell-permeable calcium chelator. This EGTA derivative is used for adjusting calcium concentrations in cells and tissues. EGTA is an aminopolycarboxylic acid, a chelating agent. Compared to EDTA, EGTA has a lower affinity for magnesium, making it more selective for calcium ions. It is useful in buffer solutions that resemble the environment in living cells where calcium ions are usually at least a thousand fold less concentrated than magnesium. The pKa for binding of calcium ions by tetrabasic EGTA is 11.00, but the protonated forms do not significantly contribute to binding, so at pH 7, the apparent pKa becomes 6.91.

Citations

View all 7 citations: Citation Explorer

Dual ablation of the RyR2-Ser2808 and RyR2-Ser2814 sites increases propensity for pro-arrhythmic spontaneous Ca2+ releases
Authors: Janicek, Radoslav and Camors, Emmanuel M and Potenza, Duilio M and Fernandez-Tenorio, Miguel and Zhao, Yanting and Dooge, Holly C and Loaiza, Randall and Alvarado, Francisco J and Egger, Marcel and Valdivia, Hector H and others,
Journal: The Journal of Physiology (2024)

Vesicular Glutamate Transporter Expression Ensures High-Fidelity Synaptic Transmission at the Calyx of Held Synapses
Authors: Nakakubo, Yutaro and Abe, Saeka and Yoshida, Tomofumi and Takami, Chihiro and Isa, Masayuki and Wojcik, Sonja M and Brose, Nils and Takamori, Shigeo and Hori, Tetsuya
Journal: Cell Reports (2020): 108040

TBC1D9 regulates TBK1 activation through Ca 2+ signaling in selective autophagy
Authors: Nozawa, Takashi and Sano, Shunsuke and Minowa-Nozawa, Atsuko and Toh, Hirotaka and Nakajima, Shintaro and Murase, Kazunori and Aikawa, Chihiro and Nakagawa, Ichiro
Journal: Nature communications (2020): 1--16

Distortion of the actin A-triad results in contractile disinhibition and cardiomyopathy
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell reports (2017): 2612--2625

Distortion of the Actin A-Triad Results in Contractile Disinhibition and Cardiomyopathy
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell Reports (2017): 2612--2625

References

View all 77 references: Citation Explorer

Tuning caged calcium: photolabile analogues of EGTA with improved optical and chelation properties
Authors: Ellis-Davies GC, Barsotti RJ.
Journal: Cell Calcium (2006): 75

Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG
Authors: Fisher AE, Hague TA, Clarke CL, Naughton DP.
Journal: Biochem Biophys Res Commun (2004): 163

Voltage-activated calcium signals in myotubes loaded with high concentrations of EGTA
Authors: Schuhmeier RP, Dietze B, Ursu D, Lehmann-Horn F, Melzer W.
Journal: Biophys J (2003): 1065

Gravicurvature loss, changes in ultrastructure and calcium balance of pea root statocytes treated with EGTA
Authors: Belyavskaya NA., undefined
Journal: J Gravit Physiol (2001): P33

L-type calcium channel blockers and EGTA enhance superoxide production in cardiac fibroblasts
Authors: Shivakumar K, Kumaran C.
Journal: J Mol Cell Cardiol (2001): 373

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