EGTA AM CAS 99590-86-0

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Physical properties

Molecular weight	668.6
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12352200

Alternative formats

EGTA AM *10 mM DMSO solution*

Overview SDS Protocol

CAS

99590-86-0

Molecular weight

668.6

EGTA AM is cell-permeable version of EGTA (ethylene glycol tetraacetic acid), a cell-permeable calcium chelator. This EGTA derivative is used for adjusting calcium concentrations in cells and tissues. EGTA is an aminopolycarboxylic acid, a chelating agent. Compared to EDTA, EGTA has a lower affinity for magnesium, making it more selective for calcium ions. It is useful in buffer solutions that resemble the environment in living cells where calcium ions are usually at least a thousand fold less concentrated than magnesium. The pKa for binding of calcium ions by tetrabasic EGTA is 11.00, but the protonated forms do not significantly contribute to binding, so at pH 7, the apparent pKa becomes 6.91.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EGTA AM *CAS 99590-86-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	149.566 µL	747.831 µL	1.496 mL	7.478 mL	14.957 mL
5 mM	29.913 µL	149.566 µL	299.133 µL	1.496 mL	2.991 mL
10 mM	14.957 µL	74.783 µL	149.566 µL	747.831 µL	1.496 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Product Family

Name	Excitation (nm)	Emission (nm)
BCECF, AM	504	527
BCECF, AM UltraPure grade	504	527
Zinquin AM UltraPure grade	355	491
Calcein, AM CAS 148504-34-1	501	521
Calcein, AM UltraPure grade CAS 148504-34-1	501	521

Images

Figure 1. Chemical structure for EGTA AM *CAS 99590-86-0*

Citations

View all 6 citations: Citation Explorer

Vesicular Glutamate Transporter Expression Ensures High-Fidelity Synaptic Transmission at the Calyx of Held Synapses
Authors: Nakakubo, Yutaro and Abe, Saeka and Yoshida, Tomofumi and Takami, Chihiro and Isa, Masayuki and Wojcik, Sonja M and Brose, Nils and Takamori, Shigeo and Hori, Tetsuya
Journal: Cell Reports (2020): 108040

TBC1D9 regulates TBK1 activation through Ca 2+ signaling in selective autophagy
Authors: Nozawa, Takashi and Sano, Shunsuke and Minowa-Nozawa, Atsuko and Toh, Hirotaka and Nakajima, Shintaro and Murase, Kazunori and Aikawa, Chihiro and Nakagawa, Ichiro
Journal: Nature communications (2020): 1--16

Distortion of the Actin A-Triad Results in Contractile Disinhibition and Cardiomyopathy
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell Reports (2017): 2612--2625

Distortion of the actin A-triad results in contractile disinhibition and cardiomyopathy
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell reports (2017): 2612--2625

Routes of Ca2+ Shuttling during Ca2+ Oscillations FOCUS ON THE ROLE OF MITOCHONDRIAL Ca2+ HANDLING AND CYTOSOLIC Ca2+ BUFFERS
Authors: Pecze, László and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230

Routes of Ca2+ shuttling during Ca2+ oscillations: Focus on the role of mitochondrial Ca2+ handling and cytosolic Ca2+ buffers
Authors: Pecze, L{\'a}szl{\'o} and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230

References

View all 77 references: Citation Explorer

Tuning caged calcium: photolabile analogues of EGTA with improved optical and chelation properties
Authors: Ellis-Davies GC, Barsotti RJ.
Journal: Cell Calcium (2006): 75

Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG
Authors: Fisher AE, Hague TA, Clarke CL, Naughton DP.
Journal: Biochem Biophys Res Commun (2004): 163

Voltage-activated calcium signals in myotubes loaded with high concentrations of EGTA
Authors: Schuhmeier RP, Dietze B, Ursu D, Lehmann-Horn F, Melzer W.
Journal: Biophys J (2003): 1065

Gravicurvature loss, changes in ultrastructure and calcium balance of pea root statocytes treated with EGTA
Authors: Belyavskaya NA., undefined
Journal: J Gravit Physiol (2001): P33

L-type calcium channel blockers and EGTA enhance superoxide production in cardiac fibroblasts
Authors: Shivakumar K, Kumaran C.
Journal: J Mol Cell Cardiol (2001): 373

Role of calcium in nitric oxide-induced cytotoxicity: EGTA protects mouse oligodendrocytes
Authors: Boullerne AI, Nedelkoska L, Benjamins JA.
Journal: J Neurosci Res (2001): 124

Correlation of EGTA and calcium-blocking agents on the response of the bladder to in vitro ischemia
Authors: Levin RM, Leggett R, Whitbeck C, Horan P.
Journal: Pharmacology (1999): 113

Effect of sarcoplasmic reticulum (SR) calcium content on SR calcium release elicited by small voltage-clamp depolarizations in frog cut skeletal muscle fibers equilibrated with 20 mM EGTA
Authors: Pape PC, Carrier N.
Journal: J Gen Physiol (1998): 161

Effects of partial sarcoplasmic reticulum calcium depletion on calcium release in frog cut muscle fibers equilibrated with 20 mM EGTA
Authors: Pape PC, Jong DS, Ch and ler WK., undefined
Journal: J Gen Physiol (1998): 263

Fura-2 calcium signals in skeletal muscle fibres loaded with high concentrations of EGTA
Authors: Struk A, Szucs G, Kemmer H, Melzer W.
Journal: Cell Calcium (1998): 23