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EGTA AM *CAS 99590-86-0*

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight668.6
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C)
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
99590-86-0
Molecular weight
668.6
EGTA AM is cell-permeable version of EGTA (ethylene glycol tetraacetic acid), a cell-permeable calcium chelator. This EGTA derivative is used for adjusting calcium concentrations in cells and tissues. EGTA is an aminopolycarboxylic acid, a chelating agent. Compared to EDTA, EGTA has a lower affinity for magnesium, making it more selective for calcium ions. It is useful in buffer solutions that resemble the environment in living cells where calcium ions are usually at least a thousand fold less concentrated than magnesium. The pKa for binding of calcium ions by tetrabasic EGTA is 11.00, but the protonated forms do not significantly contribute to binding, so at pH 7, the apparent pKa becomes 6.91.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EGTA AM *CAS 99590-86-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM149.566 µL747.831 µL1.496 mL7.478 mL14.957 mL
5 mM29.913 µL149.566 µL299.133 µL1.496 mL2.991 mL
10 mM14.957 µL74.783 µL149.566 µL747.831 µL1.496 mL

Molarity calculator

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Citations


View all 6 citations: Citation Explorer
Vesicular Glutamate Transporter Expression Ensures High-Fidelity Synaptic Transmission at the Calyx of Held Synapses
Authors: Nakakubo, Yutaro and Abe, Saeka and Yoshida, Tomofumi and Takami, Chihiro and Isa, Masayuki and Wojcik, Sonja M and Brose, Nils and Takamori, Shigeo and Hori, Tetsuya
Journal: Cell Reports (2020): 108040
TBC1D9 regulates TBK1 activation through Ca 2+ signaling in selective autophagy
Authors: Nozawa, Takashi and Sano, Shunsuke and Minowa-Nozawa, Atsuko and Toh, Hirotaka and Nakajima, Shintaro and Murase, Kazunori and Aikawa, Chihiro and Nakagawa, Ichiro
Journal: Nature communications (2020): 1--16
Distortion of the Actin A-Triad Results in Contractile Disinhibition and Cardiomyopathy
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell Reports (2017): 2612--2625
Distortion of the actin A-triad results in contractile disinhibition and cardiomyopathy
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell reports (2017): 2612--2625
Routes of Ca2+ Shuttling during Ca2+ Oscillations FOCUS ON THE ROLE OF MITOCHONDRIAL Ca2+ HANDLING AND CYTOSOLIC Ca2+ BUFFERS
Authors: Pecze, L&aacute;szl&oacute; and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230
Routes of Ca2+ shuttling during Ca2+ oscillations: Focus on the role of mitochondrial Ca2+ handling and cytosolic Ca2+ buffers
Authors: Pecze, L{\'a}szl{\'o} and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230

References


View all 77 references: Citation Explorer
Tuning caged calcium: photolabile analogues of EGTA with improved optical and chelation properties
Authors: Ellis-Davies GC, Barsotti RJ.
Journal: Cell Calcium (2006): 75
Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG
Authors: Fisher AE, Hague TA, Clarke CL, Naughton DP.
Journal: Biochem Biophys Res Commun (2004): 163
Voltage-activated calcium signals in myotubes loaded with high concentrations of EGTA
Authors: Schuhmeier RP, Dietze B, Ursu D, Lehmann-Horn F, Melzer W.
Journal: Biophys J (2003): 1065
Gravicurvature loss, changes in ultrastructure and calcium balance of pea root statocytes treated with EGTA
Authors: Belyavskaya NA., undefined
Journal: J Gravit Physiol (2001): P33
L-type calcium channel blockers and EGTA enhance superoxide production in cardiac fibroblasts
Authors: Shivakumar K, Kumaran C.
Journal: J Mol Cell Cardiol (2001): 373
Role of calcium in nitric oxide-induced cytotoxicity: EGTA protects mouse oligodendrocytes
Authors: Boullerne AI, Nedelkoska L, Benjamins JA.
Journal: J Neurosci Res (2001): 124
Correlation of EGTA and calcium-blocking agents on the response of the bladder to in vitro ischemia
Authors: Levin RM, Leggett R, Whitbeck C, Horan P.
Journal: Pharmacology (1999): 113
Effect of sarcoplasmic reticulum (SR) calcium content on SR calcium release elicited by small voltage-clamp depolarizations in frog cut skeletal muscle fibers equilibrated with 20 mM EGTA
Authors: Pape PC, Carrier N.
Journal: J Gen Physiol (1998): 161
Effects of partial sarcoplasmic reticulum calcium depletion on calcium release in frog cut muscle fibers equilibrated with 20 mM EGTA
Authors: Pape PC, Jong DS, Ch and ler WK., undefined
Journal: J Gen Physiol (1998): 263
Fura-2 calcium signals in skeletal muscle fibres loaded with high concentrations of EGTA
Authors: Struk A, Szucs G, Kemmer H, Melzer W.
Journal: Cell Calcium (1998): 23