AAT Bioquest

EthD-1 [Ethidium Homodimer-1]

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Physical properties
Molecular weight856.76
Spectral properties
Excitation (nm)528
Emission (nm)617
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Excitation (nm)
Emission (nm)
Ethidium Homodimer-1 (EthD-1) is a high-affinity fluorescent nucleic acid dye that binds ssDNA, dsDNA, RNA, oligonucleotides and triple helix DNA. Upon binding DNAs, its fluorescence is significantly enhanced (>30 times), and bright red fluorescence is emitted. Since EthD-1 carries a strong positive charge and cannot penetrate intact/living cell membranes, it is often used to detect dead cells. EthD-1 is generally used in combination with Calcein AM to stain dead and live cells respectively to determine cell viability and toxicity. EthD-1 is a dead cell-specific dye in all cell types (including mammals, bacteria and yeast). It might also be used for monitoring live/dead cell tests in combination with our other cell viability stains such as the superior Calcein Ultra Blue, Calcein Ultra Green, Cytocalcein, Cytotrace dyes and CytoTell dyes. AAT Bioquest offers the largest collection of fluorescent stains for monitoring cell viabilities.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EthD-1 [Ethidium Homodimer-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM116.719 µL583.594 µL1.167 mL5.836 mL11.672 mL
5 mM23.344 µL116.719 µL233.438 µL1.167 mL2.334 mL
10 mM11.672 µL58.359 µL116.719 µL583.594 µL1.167 mL

Molarity calculator

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Spectral properties

Excitation (nm)528
Emission (nm)617



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