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AAT Bioquest

EthD-1 [Ethidium Homodimer-1]

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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight856.76
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
61926-22-5
Molecular weight
856.76
Ethidium Homodimer-1 (EthD-1) is a high-affinity fluorescent nucleic acid dye that binds ssDNA, dsDNA, RNA, oligonucleotides and triple helix DNA. Upon binding DNAs, its fluorescence is significantly enhanced (>30 times), and bright red fluorescence is emitted. Since EthD-1 carries a strong positive charge and cannot penetrate intact/living cell membranes, it is often used to detect dead cells. EthD-1 is generally used in combination with Calcein AM to stain dead and live cells respectively to determine cell viability and toxicity. EthD-1 is a dead cell-specific dye in all cell types (including mammals, bacteria and yeast). It might also be used for monitoring live/dead cell tests in combination with our other cell viability stains such as the superior Calcein Ultra Blue, Calcein Ultra Green, Cytocalcein, Cytotrace dyes and CytoTell dyes. AAT Bioquest offers the largest collection of fluorescent stains for monitoring cell viabilities.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EthD-1 [Ethidium Homodimer-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM116.719 µL583.594 µL1.167 mL5.836 mL11.672 mL
5 mM23.344 µL116.719 µL233.438 µL1.167 mL2.334 mL
10 mM11.672 µL58.359 µL116.719 µL583.594 µL1.167 mL

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References


View all 50 references: Citation Explorer
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Optisol-GS Storage of Cultured Human Limbal Epithelial Cells at Ambient Temperature Is Superior to Hypothermic Storage.
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Journal: Current eye research (2020): 1497-1503
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Authors: Swift, William and Bair, Jeffrey A and Chen, Wanxue and Li, Michael and Lie, Sole and Li, Dayu and Yang, Menglu and Shatos, Marie A and Hodges, Robin R and Kolko, Miriam and Utheim, Tor P and Scott, Wendell and Dartt, Darlene A
Journal: BMJ open ophthalmology (2020): e000545
Butaphosphan and Cyanocobalamin Supplementation in Semen Extender on Chilled Boar Sperm Quality and Life Span.
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Journal: Frontiers in veterinary science (2020): 592162
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Authors: Shahsavari, Mohammad Hamed and Alves, Kele Amaral and Alves, Benner Geraldo and de Lima, Laritza Ferreira and Vizcarra, Diego Alberto Montano and Berrocal, Deysi Juana Dipaz and Silva, Luciana Mascena and da Silva, Yago Pinto and Zelinski, Mary B and de Figueiredo, José Ricardo and Moghaddam, Gholamali and Rodrigues, Ana Paula Ribeiro
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