EthD-1 [Ethidium Homodimer-1]

EthD-1

Ethidium Homodimer-1

Ethidium Homodimer-1 (EthD-1) is a high-affinity fluorescent nucleic acid dye that binds ssDNA, dsDNA, RNA, oligonucleotides and triple helix DNA. Upon binding DNAs, its fluorescence is significantly enhanced (>30 times), and bright red fluorescence is emitted. Since EthD-1 carries a strong positive charge and cannot penetrate intact/living cell membranes, it is often used to detect dead cells. EthD-1 is generally used in combination with Calcein AM to stain dead and live cells respectively to determine cell viability and toxicity. EthD-1 is a dead cell-specific dye in all cell types (including mammals, bacteria and yeast). It might also be used for monitoring live/dead cell tests in combination with our other cell viability stains such as the superior Calcein Ultra Blue, Calcein Ultra Green, Cytocalcein, Cytotrace dyes and CytoTell® dyes. AAT Bioquest offers the largest collection of fluorescent stains for monitoring cell viabilities.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17780	1 mg	Price

References

View all 50 references: Citation Explorer

Designing of spider silk proteins for human induced pluripotent stem cell-based cardiac tissue engineering.

Authors:

Esser, T U and Trossmann, V T and Lentz, S and Engel, F B and Scheibel, T

Journal:

Materials today. Bio (2021): 100114

Designing and validation of an automated ex-vivo bioreactor system for long term culture of bone.

Authors:

Dua, Rupak and Jones, Hugh and Noble, Philip C

Journal:

Bone reports (2021): 101074

The Silk Protein Sericin Promotes Viability of ARPE-19 and Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells in vitro.

Authors:

Khan, Ayyad Zartasht and Utheim, Tor Paaske and Moe, Morten Carstens and Aass, Hans Christian D and Sapkota, Dipak and Vallenari, Evan Michael and Eidet, Jon Roger

Journal:

Current eye research (2021): 504-514

Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model.

Authors:

Mamani, Javier Bustamante and Marinho, Bruna Souto and Rego, Gabriel Nery de Albuquerque and Nucci, Mariana Penteado and Alvieri, Fernando and Santos, Ricardo Silva Dos and Ferreira, João Victor Matias and Oliveira, Fernando Anselmo de and Gamarra, Lionel Fernel

Journal:

Einstein (Sao Paulo, Brazil) (2020): eAO4954

Influence of Tooth Pigmentation on H2O2 Diffusion and Its Cytotoxicity After In-office Tooth Bleaching.

Authors:

de Oliveira Duque, C C and Soares, D G and Briso, Alf and Ortecho-Zuta, U and de Oliveira Ribeiro, R A and Hebling, J and de Souza Costa, C A

Journal:

Operative dentistry (2020): 632-642

Physical properties

Molecular weight	856.76
Solvent	DMSO

Spectral properties

Excitation (nm)	528
Emission (nm)	617

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	61926-22-5

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on July 26, 2023