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EthD-1 [Ethidium Homodimer-1]
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 856.76 |
| Solvent | DMSO |
Spectral properties
| Excitation (nm) | 528 |
| Emission (nm) | 617 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
CAS 61926-22-5 | Molecular weight 856.76 | Excitation (nm) 528 | Emission (nm) 617 |
Ethidium Homodimer-1 (EthD-1) is a high-affinity fluorescent nucleic acid dye that binds ssDNA, dsDNA, RNA, oligonucleotides and triple helix DNA. Upon binding DNAs, its fluorescence is significantly enhanced (>30 times), and bright red fluorescence is emitted. Since EthD-1 carries a strong positive charge and cannot penetrate intact/living cell membranes, it is often used to detect dead cells. EthD-1 is generally used in combination with Calcein AM to stain dead and live cells respectively to determine cell viability and toxicity. EthD-1 is a dead cell-specific dye in all cell types (including mammals, bacteria and yeast). It might also be used for monitoring live/dead cell tests in combination with our other cell viability stains such as the superior Calcein Ultra Blue, Calcein Ultra Green, Cytocalcein, Cytotrace dyes and CytoTell dyes. AAT Bioquest offers the largest collection of fluorescent stains for monitoring cell viabilities.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of EthD-1 [Ethidium Homodimer-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 116.719 µL | 583.594 µL | 1.167 mL | 5.836 mL | 11.672 mL |
| 5 mM | 23.344 µL | 116.719 µL | 233.438 µL | 1.167 mL | 2.334 mL |
| 10 mM | 11.672 µL | 58.359 µL | 116.719 µL | 583.594 µL | 1.167 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 |
| DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 |
| DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 |
| JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 |
| BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - |
| DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 |
| DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 |
Images
Figure 1. Chemical structure for EthD-1 [Ethidium Homodimer-1].
References
View all 50 references: Citation Explorer
Designing of spider silk proteins for human induced pluripotent stem cell-based cardiac tissue engineering.
Authors: Esser, T U and Trossmann, V T and Lentz, S and Engel, F B and Scheibel, T
Journal: Materials today. Bio (2021): 100114
Authors: Esser, T U and Trossmann, V T and Lentz, S and Engel, F B and Scheibel, T
Journal: Materials today. Bio (2021): 100114
Designing and validation of an automated ex-vivo bioreactor system for long term culture of bone.
Authors: Dua, Rupak and Jones, Hugh and Noble, Philip C
Journal: Bone reports (2021): 101074
Authors: Dua, Rupak and Jones, Hugh and Noble, Philip C
Journal: Bone reports (2021): 101074
The Silk Protein Sericin Promotes Viability of ARPE-19 and Induced Pluripotent Stem Cell-Derived Retinal Pigment Epithelial Cells in vitro.
Authors: Khan, Ayyad Zartasht and Utheim, Tor Paaske and Moe, Morten Carstens and Aass, Hans Christian D and Sapkota, Dipak and Vallenari, Evan Michael and Eidet, Jon Roger
Journal: Current eye research (2021): 504-514
Authors: Khan, Ayyad Zartasht and Utheim, Tor Paaske and Moe, Morten Carstens and Aass, Hans Christian D and Sapkota, Dipak and Vallenari, Evan Michael and Eidet, Jon Roger
Journal: Current eye research (2021): 504-514
Magnetic hyperthermia therapy in glioblastoma tumor on-a-Chip model.
Authors: Mamani, Javier Bustamante and Marinho, Bruna Souto and Rego, Gabriel Nery de Albuquerque and Nucci, Mariana Penteado and Alvieri, Fernando and Santos, Ricardo Silva Dos and Ferreira, João Victor Matias and Oliveira, Fernando Anselmo de and Gamarra, Lionel Fernel
Journal: Einstein (Sao Paulo, Brazil) (2020): eAO4954
Authors: Mamani, Javier Bustamante and Marinho, Bruna Souto and Rego, Gabriel Nery de Albuquerque and Nucci, Mariana Penteado and Alvieri, Fernando and Santos, Ricardo Silva Dos and Ferreira, João Victor Matias and Oliveira, Fernando Anselmo de and Gamarra, Lionel Fernel
Journal: Einstein (Sao Paulo, Brazil) (2020): eAO4954
Influence of Tooth Pigmentation on H2O2 Diffusion and Its Cytotoxicity After In-office Tooth Bleaching.
Authors: de Oliveira Duque, C C and Soares, D G and Briso, Alf and Ortecho-Zuta, U and de Oliveira Ribeiro, R A and Hebling, J and de Souza Costa, C A
Journal: Operative dentistry (2020): 632-642
Authors: de Oliveira Duque, C C and Soares, D G and Briso, Alf and Ortecho-Zuta, U and de Oliveira Ribeiro, R A and Hebling, J and de Souza Costa, C A
Journal: Operative dentistry (2020): 632-642
Preclinical ex-vivo Testing of Anti-inflammatory Drugs in a Bovine Intervertebral Degenerative Disc Model.
Authors: Li, Zhen and Gehlen, Yannik and Heizmann, Fabian and Grad, Sibylle and Alini, Mauro and Richards, R Geoff and Kubosch, David and Südkamp, Norbert and Izadpanah, Kaywan and Kubosch, Eva Johanna and Lang, Gernot
Journal: Frontiers in bioengineering and biotechnology (2020): 583
Authors: Li, Zhen and Gehlen, Yannik and Heizmann, Fabian and Grad, Sibylle and Alini, Mauro and Richards, R Geoff and Kubosch, David and Südkamp, Norbert and Izadpanah, Kaywan and Kubosch, Eva Johanna and Lang, Gernot
Journal: Frontiers in bioengineering and biotechnology (2020): 583
Optisol-GS Storage of Cultured Human Limbal Epithelial Cells at Ambient Temperature Is Superior to Hypothermic Storage.
Authors: Jackson, Catherine Joan and Pasovic, Lara and Raeder, Sten and Sehic, Amer and Roald, Borghild and de la Paz, Maria F and Tønseth, Kim Alexsander and Utheim, Tor Paaske
Journal: Current eye research (2020): 1497-1503
Authors: Jackson, Catherine Joan and Pasovic, Lara and Raeder, Sten and Sehic, Amer and Roald, Borghild and de la Paz, Maria F and Tønseth, Kim Alexsander and Utheim, Tor Paaske
Journal: Current eye research (2020): 1497-1503
Povidone iodine treatment is deleterious to human ocular surface conjunctival cells in culture.
Authors: Swift, William and Bair, Jeffrey A and Chen, Wanxue and Li, Michael and Lie, Sole and Li, Dayu and Yang, Menglu and Shatos, Marie A and Hodges, Robin R and Kolko, Miriam and Utheim, Tor P and Scott, Wendell and Dartt, Darlene A
Journal: BMJ open ophthalmology (2020): e000545
Authors: Swift, William and Bair, Jeffrey A and Chen, Wanxue and Li, Michael and Lie, Sole and Li, Dayu and Yang, Menglu and Shatos, Marie A and Hodges, Robin R and Kolko, Miriam and Utheim, Tor P and Scott, Wendell and Dartt, Darlene A
Journal: BMJ open ophthalmology (2020): e000545
Butaphosphan and Cyanocobalamin Supplementation in Semen Extender on Chilled Boar Sperm Quality and Life Span.
Authors: Suwimonteerabutr, J and Chumsri, S and Tummaruk, P and Nuntapaitoon, Morakot
Journal: Frontiers in veterinary science (2020): 592162
Authors: Suwimonteerabutr, J and Chumsri, S and Tummaruk, P and Nuntapaitoon, Morakot
Journal: Frontiers in veterinary science (2020): 592162
Impacts of different synthetic polymers on vitrification of ovarian tissue.
Authors: Shahsavari, Mohammad Hamed and Alves, Kele Amaral and Alves, Benner Geraldo and de Lima, Laritza Ferreira and Vizcarra, Diego Alberto Montano and Berrocal, Deysi Juana Dipaz and Silva, Luciana Mascena and da Silva, Yago Pinto and Zelinski, Mary B and de Figueiredo, José Ricardo and Moghaddam, Gholamali and Rodrigues, Ana Paula Ribeiro
Journal: Cryobiology (2020): 66-72
Authors: Shahsavari, Mohammad Hamed and Alves, Kele Amaral and Alves, Benner Geraldo and de Lima, Laritza Ferreira and Vizcarra, Diego Alberto Montano and Berrocal, Deysi Juana Dipaz and Silva, Luciana Mascena and da Silva, Yago Pinto and Zelinski, Mary B and de Figueiredo, José Ricardo and Moghaddam, Gholamali and Rodrigues, Ana Paula Ribeiro
Journal: Cryobiology (2020): 66-72