EthD-2 [Ethidium Homodimer-2]

EthD-2

Ethidium Homodimer-2

Ethidium Homodimer-2 (EthD-2) is usually used as a cell-impermeant viability indicator. It is a high-affinity nucleic acid stain that is weakly fluorescent until bound to DNA and emits red fluorescence (excitation/emission maxima ~535/624). It is often used together with Calcein AM. The viability of cells can be readily assessed by staining with calcein acetoxymethyl ester (Calcein AM) and EthD-2. The live cells (green, stained with Calcein AM) and dead cells (red, stained with EthD-2) can be easily imaged and even quantified from the images using the ImageJ program. It might also be combined with our other cell viability stains such as the superior Calcein Ultra Blue, Calcein Ultra Green, Cytocalcein, Cytotrace dyes and CytoTell® dyes. AAT Bioquest offers the largest collection of fluorescent stains for monitoring cell viabilities.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
17782	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EthD-2 [Ethidium Homodimer-2] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	77.357 µL	386.784 µL	773.569 µL	3.868 mL	7.736 mL
5 mM	15.471 µL	77.357 µL	154.714 µL	773.569 µL	1.547 mL
10 mM	7.736 µL	38.678 µL	77.357 µL	386.784 µL	773.569 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1292.71 g/mol	=	mL	x	mM	=	mmol

References

View all 15 references: Citation Explorer

Iatrogenic Articular Cartilage Injury in Arthroscopic Hip and Knee Videos and the Potential for Cartilage Cell Death When Simulated in a Bovine Model.

Authors:

Compton, Jocelyn and Slattery, Michael and Coleman, Mitchell and Westermann, Robert

Journal:

Arthroscopy : the journal of arthroscopic & related surgery : official publication of the Arthroscopy Association of North Ameri (2020): 2114-2121

In Vitro Effect of Vancomycin on the Viability of Articular Chondrocytes.

Authors:

Shaw, K Aaron and Eichinger, Josef K and Nadig, Nischal and Parada, Stephen A

Journal:

Journal of orthopaedic trauma (2018): 148-153

Chondrocyte Viability After a Simulated Blast Exposure.

Authors:

Shaw, K Aaron and Johnson, Peter C and Williams, David and Zumbrun, Steven D and Topolski, Richard and Cameron, Craig D

Journal:

Military medicine (2017): e1941-e1947

Why Do Osteochondral Allografts Survive? Comparative Analysis of Cartilage Biochemical Properties Unveils a Molecular Basis for Durability.

Authors:

Ding, Lei and Zampogna, Biagio and Vasta, Sebastiano and Jang, Kee Woong and De Caro, Francesca and Martin, James A and Amendola, Annunziato

Journal:

The American journal of sports medicine (2015): 2459-68

DNA counterstaining for methylation and hydroxymethylation immunostaining in bovine zygotes.

Authors:

Heras, Sonia and Forier, Katrien and Rombouts, Koen and Braeckmans, Kevin and Van Soom, Ann

Journal:

Analytical biochemistry (2014): 14-6

Physical properties

Molecular weight	1292.71
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on July 26, 2023