FAM-cGMP PDE V substrate *Green Fluorescence*

FAM-cGMP PDE V substrate

Green Fluorescence

This green fluorescent cGMP derivative is a specific substrate for phosphodiesterase (PDE) V. It can be used for assaying PDE V activities or screening PDE V inhibitors in combination with anti-cGMP antibody in a FRET readout or FP format. PDE is a group of enzymes that degrade the second messenger molecules: cyclic nucleotides cAMP and cGMP. They regulate the localization, duration, and amplitude of cyclic nucleotide signaling within subcellular domains. PDEs are therefore important regulators of signal transduction mediated by these second messenger molecules. PDE enzymes are often targets for pharmacological inhibition due to their unique tissue distribution, structural and functional properties. Inhibitors of PDE can prolong or enhance the effects of physiological processes mediated by cAMP or cGMP by inhibition of their degradation by PDE. PDE inhibitors have been identified as new potential therapeutics in areas such as pulmonary arterial hypertension, coronary heart disease, dementia, depression and schizophrenia. For example, Sildenafil (Viagra) is an inhibitor of cGMP-specific PDE V, which enhances the vasodilatory effects of cGMP in the corpus cavernosum, and is used to treat erectile dysfunction.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
13604	0.5 umol	Price

References

View all 34 references: Citation Explorer

Importance of cAMP-response element-binding protein in regulation of expression of the murine cyclic nucleotide phosphodiesterase 3B (Pde3b) gene in differentiating 3T3-L1 preadipocytes

Authors:

Liu H, Tang JR, Choi YH, Napolitano M, Hockman S, Taira M, Degerman E, Manganiello VC.

Journal:

J Biol Chem (2006): 21096

Immunohistochemical distribution of cAMP- and cGMP-Phosphodiesterase (PDE) isoenzymes in the human prostate

Authors:

Sampaio FJ., undefined

Journal:

Int Braz J Urol (2006): 368

Scanning peptide array analyses identify overlapping binding sites for the signalling scaffold proteins, beta-arrestin and RACK1, in cAMP-specific phosphodiesterase PDE4D5

Authors:

Bolger GB, Baillie GS, Li X, Lynch MJ, Herzyk P, Mohamed A, Mitchell LH, McCahill A, Hundsrucker C, Klussmann E, Adams DR, Houslay MD.

Journal:

Biochem J (2006): 23

cAMP phosphodiesterase-4A1 (PDE4A1) has provided the paradigm for the intracellular targeting of phosphodiesterases, a process that underpins compartmentalized cAMP signalling

Authors:

Huston E, Houslay TM, Baillie GS, Houslay MD.

Journal:

Biochem Soc Trans (2006): 504

Expression and Activity of cAMP Phosphodiesterase Isoforms in Pulmonary Artery Smooth Muscle Cells from Patients with Pulmonary Hypertension: Role for PDE1

Authors:

Murray F, Patel HH, Suda RY, Zhang S, Thistlethwaite P, Yuan JX, Insel PA.

Journal:

Am J Physiol Lung Cell Mol Physiol. (2006)

Physical properties

Molecular weight	~800
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.178
Extinction coefficient (cm ^-1 M ^-1)	83000
Excitation (nm)	493
Emission (nm)	517

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	525 nm
Cutoff	515 nm
Recommended plate	Solid black

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 18, 2025