FastClick™ 5-TAMRA Alkyne
FastClick™ 5-TAMRA Alkyne contains both the moiety of FastClick (for assisting click efficiency) and 5-TAMRA fluorophore (as the fluorescence tag) for developing 5-TAMRA-based fluorescent probes. It readily reacts with an azido-modified biomolecule under extremely mild conditions. 5-TAMRA is one of the most common orange-red fluorophores used for labeling biomolecules, in particular, for labeling peptides. 5-TAMRA conjugates are widely used in fluorescence-based biological detections such as for developing FRET protease substrates. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.
References
View all 27 references: Citation Explorer
Monitoring regional astrocyte diversity by cell type-specific proteomic labeling in vivo.
Authors: Prabhakar, Priyadharshini and Pielot, Rainer and Landgraf, Peter and Wissing, Josef and Bayrhammer, Anne and van Ham, Marco and Gundelfinger, Eckart D and Jänsch, Lothar and Dieterich, Daniela C and Müller, Anke
Journal: Glia (2023): 682-703
Authors: Prabhakar, Priyadharshini and Pielot, Rainer and Landgraf, Peter and Wissing, Josef and Bayrhammer, Anne and van Ham, Marco and Gundelfinger, Eckart D and Jänsch, Lothar and Dieterich, Daniela C and Müller, Anke
Journal: Glia (2023): 682-703
trans-Cyclooctene- and Bicyclononyne-Linked Nucleotides for Click Modification of DNA with Fluorogenic Tetrazines and Live Cell Metabolic Labeling and Imaging.
Authors: Spampinato, Ambra and Kužmová, Erika and Pohl, Radek and Sýkorová, Veronika and Vrábel, Milan and Kraus, Tomáš and Hocek, Michal
Journal: Bioconjugate chemistry (2023): 772-80
Authors: Spampinato, Ambra and Kužmová, Erika and Pohl, Radek and Sýkorová, Veronika and Vrábel, Milan and Kraus, Tomáš and Hocek, Michal
Journal: Bioconjugate chemistry (2023): 772-80
Divergent Synthesis of Ultrabright and Dendritic Xanthenes for Enhanced Click-Chemistry-Based Bioimaging.
Authors: Montiel, Luis and Spada, Fabio and Crisp, Antony and Serdjukow, Sascha and Carell, Thomas and Frischmuth, Thomas
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202202633
Authors: Montiel, Luis and Spada, Fabio and Crisp, Antony and Serdjukow, Sascha and Carell, Thomas and Frischmuth, Thomas
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202202633
Identification and Profiling of Histone Acetyltransferase Substrates by Bioorthogonal Labeling.
Authors: Song, Jiabao and Han, Zhen and Zheng, Y George
Journal: Current protocols (2022): e497
Authors: Song, Jiabao and Han, Zhen and Zheng, Y George
Journal: Current protocols (2022): e497
Synthesis and characterization of abscisic acid receptor modulators.
Authors: Vaidya, Aditya S and Park, Sang-Youl and Xing, Zenan and Cutler, Sean R
Journal: Methods in enzymology (2022): 435-470
Authors: Vaidya, Aditya S and Park, Sang-Youl and Xing, Zenan and Cutler, Sean R
Journal: Methods in enzymology (2022): 435-470
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