FastClick™ 5-TAMRA Alkyne
Ordering information
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Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 738.89 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
FastClick™ 5-TAMRA Azide |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 738.89 |
FastClick™ 5-TAMRA Alkyne contains both the moiety of FastClick (for assisting click efficiency) and 5-TAMRA fluorophore (as the fluorescence tag) for developing 5-TAMRA-based fluorescent probes. It readily reacts with an azido-modified biomolecule under extremely mild conditions. 5-TAMRA is one of the most common orange-red fluorophores used for labeling biomolecules, in particular, for labeling peptides. 5-TAMRA conjugates are widely used in fluorescence-based biological detections such as for developing FRET protease substrates. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ 5-TAMRA Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 135.338 µL | 676.691 µL | 1.353 mL | 6.767 mL | 13.534 mL |
5 mM | 27.068 µL | 135.338 µL | 270.676 µL | 1.353 mL | 2.707 mL |
10 mM | 13.534 µL | 67.669 µL | 135.338 µL | 676.691 µL | 1.353 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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Images
Figure 1. The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
References
View all 27 references: Citation Explorer
Monitoring regional astrocyte diversity by cell type-specific proteomic labeling in vivo.
Authors: Prabhakar, Priyadharshini and Pielot, Rainer and Landgraf, Peter and Wissing, Josef and Bayrhammer, Anne and van Ham, Marco and Gundelfinger, Eckart D and Jänsch, Lothar and Dieterich, Daniela C and Müller, Anke
Journal: Glia (2023): 682-703
Authors: Prabhakar, Priyadharshini and Pielot, Rainer and Landgraf, Peter and Wissing, Josef and Bayrhammer, Anne and van Ham, Marco and Gundelfinger, Eckart D and Jänsch, Lothar and Dieterich, Daniela C and Müller, Anke
Journal: Glia (2023): 682-703
trans-Cyclooctene- and Bicyclononyne-Linked Nucleotides for Click Modification of DNA with Fluorogenic Tetrazines and Live Cell Metabolic Labeling and Imaging.
Authors: Spampinato, Ambra and Kužmová, Erika and Pohl, Radek and Sýkorová, Veronika and Vrábel, Milan and Kraus, Tomáš and Hocek, Michal
Journal: Bioconjugate chemistry (2023): 772-80
Authors: Spampinato, Ambra and Kužmová, Erika and Pohl, Radek and Sýkorová, Veronika and Vrábel, Milan and Kraus, Tomáš and Hocek, Michal
Journal: Bioconjugate chemistry (2023): 772-80
Divergent Synthesis of Ultrabright and Dendritic Xanthenes for Enhanced Click-Chemistry-Based Bioimaging.
Authors: Montiel, Luis and Spada, Fabio and Crisp, Antony and Serdjukow, Sascha and Carell, Thomas and Frischmuth, Thomas
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202202633
Authors: Montiel, Luis and Spada, Fabio and Crisp, Antony and Serdjukow, Sascha and Carell, Thomas and Frischmuth, Thomas
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2023): e202202633
Identification and Profiling of Histone Acetyltransferase Substrates by Bioorthogonal Labeling.
Authors: Song, Jiabao and Han, Zhen and Zheng, Y George
Journal: Current protocols (2022): e497
Authors: Song, Jiabao and Han, Zhen and Zheng, Y George
Journal: Current protocols (2022): e497
Synthesis and characterization of abscisic acid receptor modulators.
Authors: Vaidya, Aditya S and Park, Sang-Youl and Xing, Zenan and Cutler, Sean R
Journal: Methods in enzymology (2022): 435-470
Authors: Vaidya, Aditya S and Park, Sang-Youl and Xing, Zenan and Cutler, Sean R
Journal: Methods in enzymology (2022): 435-470
Genetically encoded dihydroxyphenylalanine coupled with tyrosinase for strain promoted labeling.
Authors: George, Augustine and Indhu, Mohan and Ashokraj, Sundarapandian and Shanmugam, Ganesh and Ganesan, Ponesakki and Kamini, Numbi Ramudu and Ayyadurai, Niraikulam
Journal: Bioorganic & medicinal chemistry (2021): 116460
Authors: George, Augustine and Indhu, Mohan and Ashokraj, Sundarapandian and Shanmugam, Ganesh and Ganesan, Ponesakki and Kamini, Numbi Ramudu and Ayyadurai, Niraikulam
Journal: Bioorganic & medicinal chemistry (2021): 116460
Click-to-lead design of a picomolar ABA receptor antagonist with potent activity in vivo.
Authors: Vaidya, Aditya S and Peterson, Francis C and Eckhardt, James and Xing, Zenan and Park, Sang-Youl and Dejonghe, Wim and Takeuchi, Jun and Pri-Tal, Oded and Faria, Julianna and Elzinga, Dezi and Volkman, Brian F and Todoroki, Yasushi and Mosquna, Assaf and Okamoto, Masanori and Cutler, Sean R
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Authors: Vaidya, Aditya S and Peterson, Francis C and Eckhardt, James and Xing, Zenan and Park, Sang-Youl and Dejonghe, Wim and Takeuchi, Jun and Pri-Tal, Oded and Faria, Julianna and Elzinga, Dezi and Volkman, Brian F and Todoroki, Yasushi and Mosquna, Assaf and Okamoto, Masanori and Cutler, Sean R
Journal: Proceedings of the National Academy of Sciences of the United States of America (2021)
Direct and specific binding of cholesterol to the mitochondrial translocator protein (TSPO) using PhotoClick cholesterol analogue.
Authors: Georges, Elias and Sottas, Chantal and Li, Yuchang and Papadopoulos, Vassilios
Journal: Journal of biochemistry (2021): 239-243
Authors: Georges, Elias and Sottas, Chantal and Li, Yuchang and Papadopoulos, Vassilios
Journal: Journal of biochemistry (2021): 239-243
Profiling and Validation of Live-Cell Protein Methylation with Engineered Enzymes and Methionine Analogues.
Authors: Weiss, Nicole and Seneviranthe, Chamara and Jiang, Ming and Wang, Ke and Luo, Minkui
Journal: Current protocols (2021): e213
Authors: Weiss, Nicole and Seneviranthe, Chamara and Jiang, Ming and Wang, Ke and Luo, Minkui
Journal: Current protocols (2021): e213
Azide-Functionalized Naphthoxyloside as a Tool for Glycosaminoglycan Investigations.
Authors: Willén, Daniel and Mastio, Roberto and Söderlund, Zackarias and Manner, Sophie and Westergren-Thorsson, Gunilla and Tykesson, Emil and Ellervik, Ulf
Journal: Bioconjugate chemistry (2021): 2507-2515
Authors: Willén, Daniel and Mastio, Roberto and Söderlund, Zackarias and Manner, Sophie and Westergren-Thorsson, Gunilla and Tykesson, Emil and Ellervik, Ulf
Journal: Bioconjugate chemistry (2021): 2507-2515