FastClick™ 5-TAMRA Azide
Ordering information
Price | |
Catalog Number | |
Unit Size | |
Quantity |
Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 642.72 |
Solvent | DMSO |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
FastClick™ 5-TAMRA Alkyne |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 642.72 |
FastClick™ 5-TAMRA Azide contains both the CAG moiety of FastClick (for assisting click efficiency) and 5-TAMRA fluorophore (as the fluorescence tag) for developing 5-TAMRA-based fluorescent probes. 5-TAMRA azide is one of the most widely used orange fluorophores, in particular, for labeling alkyne-modified peptides and other small biomolecules. It has almost the identical fluorescence spectra to Alexa Fluor 546, a rhodamine analog. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ 5-TAMRA Azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 155.589 µL | 777.944 µL | 1.556 mL | 7.779 mL | 15.559 mL |
5 mM | 31.118 µL | 155.589 µL | 311.177 µL | 1.556 mL | 3.112 mL |
10 mM | 15.559 µL | 77.794 µL | 155.589 µL | 777.944 µL | 1.556 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Images
Figure 1. FastClick™ 5-TAMRA Azide contains both the CAG moiety of FastClick (for assisting click efficiency) and 5-TAMRA fluorophore (as the fluorescence tag) for developing 5-TAMRA-based fluorescent probes. 5-TAMRA azide is one of the most widely used orange fluorophores, in particular, for labeling alkyne-modified peptides and other small biomolecules.
Figure 2. The reaction (Green Bar) of FastClick Cy5 Azide with coumarin alkyne occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 azide does not effectively react with the coumarin alkyne substrate.
References
View all 5 references: Citation Explorer
Direct and specific binding of cholesterol to the mitochondrial translocator protein (TSPO) using PhotoClick cholesterol analogue.
Authors: Georges, Elias and Sottas, Chantal and Li, Yuchang and Papadopoulos, Vassilios
Journal: Journal of biochemistry (2021): 239-243
Authors: Georges, Elias and Sottas, Chantal and Li, Yuchang and Papadopoulos, Vassilios
Journal: Journal of biochemistry (2021): 239-243
Profiling and Validation of Live-Cell Protein Methylation with Engineered Enzymes and Methionine Analogues.
Authors: Weiss, Nicole and Seneviranthe, Chamara and Jiang, Ming and Wang, Ke and Luo, Minkui
Journal: Current protocols (2021): e213
Authors: Weiss, Nicole and Seneviranthe, Chamara and Jiang, Ming and Wang, Ke and Luo, Minkui
Journal: Current protocols (2021): e213
New chemical tools for probing activity and inhibition of the NAD+-dependent lysine deacylase sirtuin 2.
Authors: Swyter, Sören and Schiedel, Matthias and Monaldi, Daria and Szunyogh, Sándor and Lehotzky, Attila and Rumpf, Tobias and Ovádi, Judit and Sippl, Wolfgang and Jung, Manfred
Journal: Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2018)
Authors: Swyter, Sören and Schiedel, Matthias and Monaldi, Daria and Szunyogh, Sándor and Lehotzky, Attila and Rumpf, Tobias and Ovádi, Judit and Sippl, Wolfgang and Jung, Manfred
Journal: Philosophical transactions of the Royal Society of London. Series B, Biological sciences (2018)
Liposome functionalization with copper-free "click chemistry".
Authors: Oude Blenke, Erik and Klaasse, Gruson and Merten, Hannes and Plückthun, Andreas and Mastrobattista, Enrico and Martin, Nathaniel I
Journal: Journal of controlled release : official journal of the Controlled Release Society (2015): 14-20
Authors: Oude Blenke, Erik and Klaasse, Gruson and Merten, Hannes and Plückthun, Andreas and Mastrobattista, Enrico and Martin, Nathaniel I
Journal: Journal of controlled release : official journal of the Controlled Release Society (2015): 14-20
Development of clickable active site-directed photoaffinity probes for γ-secretase.
Authors: Crump, Christina J and am Ende, Christopher W and Ballard, T Eric and Pozdnyakov, Nikolay and Pettersson, Martin and Chau, De-Ming and Bales, Kelly R and Li, Yue-Ming and Johnson, Douglas S
Journal: Bioorganic & medicinal chemistry letters (2012): 2997-3000
Authors: Crump, Christina J and am Ende, Christopher W and Ballard, T Eric and Pozdnyakov, Nikolay and Pettersson, Martin and Chau, De-Ming and Bales, Kelly R and Li, Yue-Ming and Johnson, Douglas S
Journal: Bioorganic & medicinal chemistry letters (2012): 2997-3000