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FastClick™ Click Reaction Buffer
Product key features
The FastClick™ Click Reaction Buffer provides a streamlined and efficient solution for performing copper-catalyzed click reactions in chemical and synthetic workflows.
- High-efficiency click coupling: Supports CuAAC for reliable and high-yield molecular conjugation.
- Simplified two-component system: Pre-optimized buffers reduce preparation steps and ensure consistent reaction performance.
- Versatile compatibility: Ideal for a wide range of chemical labeling and ligation applications including small molecules, polymers, and material surface modifications.
Product description
The FastClick™ Click Reaction Buffer Kit is a market-first solution for chemists seeking a fast, reproducible, and scalable method for copper-catalyzed azide-alkyne cycloaddition (CuAAC) in synthetic systems. This kit includes two components that enable straightforward setup and highly efficient click reactions.
CuAAC is widely recognized as a reliable and orthogonal ligation method for generating complex molecules, attaching tags, and linking functional groups. The FastClick™ buffer system facilitates this process by delivering robust performance across a range of commonly used organic and aqueous solvents, substrates, and surfaces. With a convenient mix-and-use format and 50 mL pack size, this kit is well-suited for both bench-scale reactions and larger preparative work in chemical synthesis, materials science, drug development, and surface functionalization.
Example protocol
AT A GLANCE
- Prepare 50 µL azide- and alkyne- functionalized molecules solution mix.
- Add 50 µL 2X Click Reaction Mix, and mix briefly.
- Add 5 µL 20X Click Reaction Additive stock solution to start the reaction.
- Incubate samples for 20-30 min at room temperature (protected from light).
- Alkyne-functionalized biomolecules and azide-functionalized biomolecules are now click-labeled and ready for downstream process.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 1 mL of water to one vial of Click Reaction Additive (Component B) to make a 20X additive stock solution.
Note 1: The 20X Click Reaction Additive stock solution is oxidized easily, use it promptly. Prepare single use aliquots, and store at -20 °C. Avoid repeated freeze-thaw-cycles.
Note 2: Do not use solutions that appears dark brown. Freshly prepared 20X Click Reaction Additive stock solution is colorless to slightly yellow. It loses its reduction capability when it turns dark brown upon oxidation.
SAMPLE EXPERIMENTAL PROTOCOL
- Prepare 50 µL azide- and alkyne- functionalized molecules solution mix in PBS or appropriate buffer.
- Add 50 µL of 2X Click Reaction Mix (Component A), and mix briefly.
- Add 5 µL of 20X Click Reaction Additive stock solution to start the reaction.
- Incubate samples for 20-30 minutes at room temperature (protected from light).
- Alkyne-functionalized biomolecules and azide-functionalized biomolecules are now click-labeled and ready for downstream processes.
Note: The volume of the click reaction can be scaled-up proportionally.
References
Authors: Sombat, Witsanu and Padungros, Panuwat and Hoven, Voravee P
Journal: Langmuir : the ACS journal of surfaces and colloids (2025): 6729-6739
Authors: Imamura, Ryota and Takasu, Akinori
Journal: RSC advances (2025): 12001-12008
Authors: Wang, Dinghai and He, Zhiyong and Liu, Chang and Wu, Yuting and Tang, Zhongkuan and Liu, Siqi and Yu, Baolin and Luo, Jingwen and Li, Bindong
Journal: Organic letters (2025): 4000-4005
Authors: Gao, Zhongshuai and Zhang, Shuya and Duan, Yanyi and Chang, Heng and Cui, Mei and Huang, Renliang and Su, Rongxin
Journal: ACS applied materials & interfaces (2025): 8299-8311
Authors: Kelemen, Viktor and Bege, Miklós and Debreczeni, Nóra and Bereczki, Ilona and Bényei, Attila Csaba and Herczegh, Pál and Borbás, Anikó
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2025): e202500104
Safety data sheet