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FastClick™ Click Reaction Buffer Kit
Product key features
The FastClick™ Click Reaction Buffer Kit offers a fast and convenient method for performing copper-catalyzed click reactions in biological samples.
- Efficient click labeling: Facilitates CuAAC for fast and efficient bioconjugation of biomolecules.
- Two-component buffer system: Includes pre-optimized reagents for controlled and reproducible click reaction conditions in biological samples.
- Broad biological compatibility: Suitable for protein and nucleic acid labeling, biomolecular conjugation, and other biochemistry workflows.
Product description
The FastClick™ Click Reaction Buffer Kit is a market-first solution for a convenient copper-catalyzed azide-alkyne cycloaddition (CuAAC) in biological systems. This kit features two components in a mix-and-use format that enables rapid and specific bioconjugation under mild conditions.
The CuAAC click reaction is a gold-standard bioorthogonal coupling method, offering exceptional specificity due to the low natural abundance of azide and alkyne groups in biological systems. This kit is designed for biological research applications, making it ideal for supporting high-efficiency bioconjugation workflows such as protein labeling, nucleic acid modification, and molecular tagging. The reaction is quick and produces reliable, high-yield results with minimal background interference. With its simple protocol and robust performance, the FastClick™ buffer kit is well-suited for cell biology, proteomics, and biochemical studies involving click chemistry in complex biological samples.
Example protocol
AT A GLANCE
- Prepare 50 µL azide- and alkyne- functionalized molecules solution mix.
- Add 50 µL 2X Click Reaction Mix, and mix briefly.
- Add 5 µL 20X Click Reaction Additive stock solution to start the reaction.
- Incubate samples for 20-30 min at room temperature (protected from light).
- Alkyne-functionalized biomolecules and azide-functionalized biomolecules are now click-labeled and ready for downstream process.
PREPARATION OF STOCK SOLUTIONS
Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles
Add 125 µL of water to one vial of Click Reaction Additive (Component B) to make a 20X Additive stock solution.
Note 1: The 20X Click Reaction Additive stock solution is oxidized easily, use it promptly. Prepare single use aliquots, and store at -20 °C. Avoid repeated freeze-thaw-cycles.
Note 2: Do not use solutions that appears dark brown. Freshly prepared 20X Click Reaction Additive stock solution is colorless to slightly yellow. It loses its reduction capability when it turns dark brown upon oxidation.
SAMPLE EXPERIMENTAL PROTOCOL
- Prepare 50 µL azide- and alkyne- functionalized molecules solution mix in PBS or appropriate buffer.
- Add 50 µL of 2X Click Reaction Mix (Component A), and mix briefly.
- Add 5 µL of 20X Click Reaction Additive stock solution to start the reaction.
- Incubate samples for 20-30 minutes at room temperature (protected from light).
- Alkyne-functionalized biomolecules and azide-functionalized biomolecules are now click-labeled and ready for downstream processes.
References
Authors: Sombat, Witsanu and Padungros, Panuwat and Hoven, Voravee P
Journal: Langmuir : the ACS journal of surfaces and colloids (2025): 6729-6739
Authors: Imamura, Ryota and Takasu, Akinori
Journal: RSC advances (2025): 12001-12008
Authors: Wang, Dinghai and He, Zhiyong and Liu, Chang and Wu, Yuting and Tang, Zhongkuan and Liu, Siqi and Yu, Baolin and Luo, Jingwen and Li, Bindong
Journal: Organic letters (2025): 4000-4005
Authors: Gao, Zhongshuai and Zhang, Shuya and Duan, Yanyi and Chang, Heng and Cui, Mei and Huang, Renliang and Su, Rongxin
Journal: ACS applied materials & interfaces (2025): 8299-8311
Authors: Kelemen, Viktor and Bege, Miklós and Debreczeni, Nóra and Bereczki, Ilona and Bényei, Attila Csaba and Herczegh, Pál and Borbás, Anikó
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2025): e202500104
Safety data sheet