FastClick™ Cy3 Alkyne
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 939.20 |
Solvent | DMSO |
Spectral properties
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.073 |
Extinction coefficient (cm -1 M -1) | 1500001 |
Excitation (nm) | 555 |
Emission (nm) | 569 |
Quantum yield | 0.151 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
Alternative formats
FastClick™ Cy3 Azide |
Overview | SDSProtocol |
See also: Click Chemistry
Molecular weight 939.20 | Correction Factor (260 nm) 0.07 | Correction Factor (280 nm) 0.073 | Extinction coefficient (cm -1 M -1) 1500001 | Excitation (nm) 555 | Emission (nm) 569 | Quantum yield 0.151 |
FastClick™ Cy3 Alkyne contains both the moiety of FastClick (for assisting click efficiency) and Cy3 fluorophore (as the fluorescence tag) for developing Cy3-based fluorescent probes. It reacts with an azido-modified biomolecule under extremely mild conditions. Cy3 is a common orange-red fluorophore in combination with Cy5 (red fluorescence) for dual color detections. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FastClick™ Cy3 Alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 106.474 µL | 532.368 µL | 1.065 mL | 5.324 mL | 10.647 mL |
5 mM | 21.295 µL | 106.474 µL | 212.947 µL | 1.065 mL | 2.129 mL |
10 mM | 10.647 µL | 53.237 µL | 106.474 µL | 532.368 µL | 1.065 mL |
Molarity calculator
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
Correction Factor (260 nm) | 0.07 |
Correction Factor (280 nm) | 0.073 |
Extinction coefficient (cm -1 M -1) | 1500001 |
Excitation (nm) | 555 |
Emission (nm) | 569 |
Quantum yield | 0.151 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
FastClick™ Cy5 Alkyne | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
FastClick™ Cy7 Alkyne | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
FastClick™ XFD350 Alkyne | 343 | 441 | 19000 | - | 0.25 | 0.19 |
FastClick™ XFD488 Alkyne | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
FastClick™ XFD555 Alkyne | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
FastClick™ XFD647 Alkyne | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
FastClick™ XFD750 Alkyne | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
Images
Figure 1. The reaction (Green Bar) of FastClick Cy5 Alkyne with coumarin azide occurs under extremely mild conditions (e.g., [Azide] = 0.02 mM, [Alkyne] = 0.02 mM, [CuSO4] = 0.02 mM, [Sodium Ascorbate] = 5 mM, in 100 mM HEPES) under which the common Cy5 alkyne does not effectively react with the coumarin azide substrate.
References
View all 24 references: Citation Explorer
Programmed immobilization of living cells using independent click pairs.
Authors: Zhu, Chengyuan and Takemoto, Hiroyasu and Higuchi, Yuriko and Yamashita, Fumiyoshi
Journal: Biochemical and biophysical research communications (2024): 149556
Authors: Zhu, Chengyuan and Takemoto, Hiroyasu and Higuchi, Yuriko and Yamashita, Fumiyoshi
Journal: Biochemical and biophysical research communications (2024): 149556
Effective, Rapid, and Small-Scale Bioconjugation and Purification of "Clicked" Small-Molecule DNA Oligonucleotide for Nucleic Acid Nanoparticle Functionalization.
Authors: Doe, Erwin and Hayth, Hannah L and Brumett, Ross and Khisamutdinov, Emil F
Journal: International journal of molecular sciences (2023)
Authors: Doe, Erwin and Hayth, Hannah L and Brumett, Ross and Khisamutdinov, Emil F
Journal: International journal of molecular sciences (2023)
Affinity Peptide-Tethered Suspension Hydrogel Sensor for Selective and Sensitive Detection of Influenza Virus.
Authors: Kim, Ji Hong and Jeong, Hye-Seon and Hwang, Jaehyeon and Kweon, Dae-Hyuk and Choi, Chang-Hyung and Park, Jong Pil
Journal: ACS applied materials & interfaces (2023)
Authors: Kim, Ji Hong and Jeong, Hye-Seon and Hwang, Jaehyeon and Kweon, Dae-Hyuk and Choi, Chang-Hyung and Park, Jong Pil
Journal: ACS applied materials & interfaces (2023)
Fluorescent Properties of Cyanine Dyes As a Matter of the Environment.
Authors: Fan, Fan and Povedailo, Vladimir A and Lysenko, Ivan L and Seviarynchyk, Tatsiana P and Sharko, Olga L and Mazunin, Ilya O and Shmanai, Vadim V
Journal: Journal of fluorescence (2023)
Authors: Fan, Fan and Povedailo, Vladimir A and Lysenko, Ivan L and Seviarynchyk, Tatsiana P and Sharko, Olga L and Mazunin, Ilya O and Shmanai, Vadim V
Journal: Journal of fluorescence (2023)
Hybridization-Sensitive Fluorescent Probes for DNA and RNA by a Modular "Click" Approach.
Authors: Gebhard, Julian and Hirsch, Lara and Schwechheimer, Christian and Wagenknecht, Hans-Achim
Journal: Bioconjugate chemistry (2022): 1634-1642
Authors: Gebhard, Julian and Hirsch, Lara and Schwechheimer, Christian and Wagenknecht, Hans-Achim
Journal: Bioconjugate chemistry (2022): 1634-1642
Cu(I)-Catalyzed Click Reaction-Triggered 3D DNA Walker for Constructing an "OFF-ON" Fluorescent Biosensor for Cu2+ Detection.
Authors: Wang, Zhen and Jia, Nan and Zhou, Xumei and Han, Jing and Bu, Huaiyu
Journal: ACS applied bio materials (2021): 3571-3578
Authors: Wang, Zhen and Jia, Nan and Zhou, Xumei and Han, Jing and Bu, Huaiyu
Journal: ACS applied bio materials (2021): 3571-3578
Tug-of-war: molecular dynamometers against living cells for analyzing sub-piconewton interaction of a specific protein with the cell membrane.
Authors: Liu, Huipu and Chen, Yunlong and Wang, Jiawei and Yang, Yuanjiao and Ju, Huangxian
Journal: Chemical science (2021): 14389-14395
Authors: Liu, Huipu and Chen, Yunlong and Wang, Jiawei and Yang, Yuanjiao and Ju, Huangxian
Journal: Chemical science (2021): 14389-14395
Cy3-RgIA-5727 Labels and Inhibits α9-Containing nAChRs of Cochlear Hair Cells.
Authors: Fisher, Fernando and Zhang, Yuanyuan and Vincent, Philippe F Y and Gajewiak, Joanna and Gordon, Thomas J and Glowatzki, Elisabeth and Fuchs, Paul Albert and McIntosh, J Michael
Journal: Frontiers in cellular neuroscience (2021): 697560
Authors: Fisher, Fernando and Zhang, Yuanyuan and Vincent, Philippe F Y and Gajewiak, Joanna and Gordon, Thomas J and Glowatzki, Elisabeth and Fuchs, Paul Albert and McIntosh, J Michael
Journal: Frontiers in cellular neuroscience (2021): 697560
Design, Synthesis, Conjugation, and Reactivity of Novel trans,trans-1,5-Cyclooctadiene-Derived Bioorthogonal Linkers.
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Authors: Longo, Beatrice and Zanato, Chiara and Piras, Monica and Dall'Angelo, Sergio and Windhorst, Albert D and Vugts, Danielle J and Baldassarre, Massimiliano and Zanda, Matteo
Journal: Bioconjugate chemistry (2020)
Quantitative Comparison of Dye and Ultrasmall Fluorescent Silica Core-Shell Nanoparticle Probes for Optical Super-Resolution Imaging of Model Block Copolymer Thin Film Surfaces.
Authors: Hinckley, Joshua A and Chapman, Dana V and Hedderick, Konrad R and Oleske, Katharine W and Estroff, Lara A and Wiesner, Ulrich B
Journal: ACS macro letters (2019): 1378-1382
Authors: Hinckley, Joshua A and Chapman, Dana V and Hedderick, Konrad R and Oleske, Katharine W and Estroff, Lara A and Wiesner, Ulrich B
Journal: ACS macro letters (2019): 1378-1382