FastClick™ XFD350 Alkyne
FastClick™ XFD350 Alkyne contains both the CAG moiety of FastClick (for assisting click efficiency) and Alexa Fluor® 350 fluorophore (as the fluorescence tag) for developing Alexa Fluor® 350-based fluorescent probes. FastClick™ XFD350 Alkyne readily reacts with an azido-modified biomolecule under extremely mild conditions. Alexa Fluor® 350 is a commonly used blue fluorophore for labeling proteins, nucleic acids, or other biomolecules. It has moderate photostability and excitation that matches the 350 nm laser line. Alexa Fluor® is a trademark of ThermoFisher Scientific. FastClick™ reagents have been developed by the scientists of AAT Bioquest for enhancing the yield and reaction speed of copper-catalyzed azide-alkyne cycloaddition (CuAAC) reaction. They contain a copper-chelating ligand that significantly stabilizes the Cu(I) oxidation state and thus accelerates the click reaction. They do not require the use of an external copper-chelator (such as the common THPTA or BTTAA). The high concentration of copper chelators is known to have a detrimental effect on DNA/RNA, thus causing biocompatibility issues. The introduction of a copper-chelating moiety at the reporter molecule allows for a dramatic raise of the effective Cu(I) concentration at the reaction site and thus accelerates the reaction. Under extremely mild conditions the FastClick™ azides and alkynes react much faster in high yield compared to the corresponding conventional CuAAC reactions. Click chemistry was developed by K. Barry Sharpless as a robust and specific method of ligating two molecules together. Two important characteristics make click chemistry attractive for assembling biomolecules. First, click reactions are bio-orthogonal, thus the click chemistry-functionalized biomolecules would not react with the natural biomolecules that lack a clickable functional group. Second, the reactions proceed with ease under mild conditions, such as at room temperature and in aqueous media.
Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
FastClick™ Cy3 Alkyne | 555 | 569 | 1500001 | 0.151 | 0.07 | 0.073 |
FastClick™ Cy5 Alkyne | 651 | 670 | 2500001 | 0.271, 0.42 | 0.02 | 0.03 |
FastClick™ Cy7 Alkyne | 756 | 779 | 250000 | 0.3 | 0.05 | 0.036 |
FastClick™ XFD488 Alkyne | 499 | 520 | 71000 | 0.921 | 0.30 | 0.11 |
FastClick™ XFD555 Alkyne | 553 | 568 | 150000 | 0.11 | 0.08 | 0.08 |
FastClick™ XFD647 Alkyne | 650 | 671 | 239000 | 0.331 | 0.00 | 0.03 |
FastClick™ XFD750 Alkyne | 752 | 776 | 240000 | 0.121 | 0.00 | 0.04 |
References
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Click chemistry approaches for developing carbonic anhydrase inhibitors and their applications.
Authors: Angeli, Andrea and Supuran, Claudiu T
Journal: Journal of enzyme inhibition and medicinal chemistry (2023): 2166503
Authors: Angeli, Andrea and Supuran, Claudiu T
Journal: Journal of enzyme inhibition and medicinal chemistry (2023): 2166503
Polydopamine nanoparticle-mediated, click chemistry triggered, microparticle-counting immunosensor for the sensitive detection of ochratoxin A.
Authors: Chen, Rui and Dong, Yongzhen and Hong, Feng and Zhang, Xiya and Wang, Xiaohong and Wang, Jia and Chen, Yiping
Journal: Journal of hazardous materials (2022): 128206
Authors: Chen, Rui and Dong, Yongzhen and Hong, Feng and Zhang, Xiya and Wang, Xiaohong and Wang, Jia and Chen, Yiping
Journal: Journal of hazardous materials (2022): 128206
Design, Synthesis and Biological Evaluation of New Carbohydrate-Based Coumarin Derivatives as Selective Carbonic Anhydrase IX Inhibitors via "Click" Reaction.
Authors: Chu, Naying and Wang, Yitong and Jia, Hao and Han, Jie and Wang, Xiaoyi and Hou, Zhuang
Journal: Molecules (Basel, Switzerland) (2022)
Authors: Chu, Naying and Wang, Yitong and Jia, Hao and Han, Jie and Wang, Xiaoyi and Hou, Zhuang
Journal: Molecules (Basel, Switzerland) (2022)
Late-Stage Functionalization through Click Chemistry Provides GLUT5-Targeting Glycoconjugate as a Potential PET Imaging Probe.
Authors: Oronova, Adelina and Tanasova, Marina
Journal: International journal of molecular sciences (2022)
Authors: Oronova, Adelina and Tanasova, Marina
Journal: International journal of molecular sciences (2022)
New Azido Coumarins as Potential Agents for Fluorescent Labeling and Their "Click" Chemistry Reactions for the Conjugation with closo-Dodecaborate Anion.
Authors: Laskova, Julia and Serdyukov, Alexander and Kosenko, Irina and Ananyev, Ivan and Titova, Ekaterina and Druzina, Anna and Sivaev, Igor and Antonets, Anastasia A and Nazarov, Alexey A and Bregadze, Vladimir I
Journal: Molecules (Basel, Switzerland) (2022)
Authors: Laskova, Julia and Serdyukov, Alexander and Kosenko, Irina and Ananyev, Ivan and Titova, Ekaterina and Druzina, Anna and Sivaev, Igor and Antonets, Anastasia A and Nazarov, Alexey A and Bregadze, Vladimir I
Journal: Molecules (Basel, Switzerland) (2022)
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