FDG [Fluorescein di-beta-D-galactopyranoside] *CAS#: 17817-20-8*

FDG

Fluorescein di-beta-D-galactopyranoside; CAS#: 17817-20-8

Although chromogenic assays of beta-galactosidase activity (i.e. X-Gal) are still widely used, the recent advance in fluorescence instrumentation has made fluorogenic substrates (such as FDG and MUG) be several orders of magnitude more sensitive. FDG is considered to be one of the most sensitive fluorogenic substrates available for detecting beta-galactosidase. The colorless and nonfluorescent FDG is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Galactosidase-catalyzed hydrolysis of FDG can be followed by fluorescence increase around 520 nm. Alternatively, FDG can also be used to detect ?-galactosidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). FDG has been widely used for identifying LacZ-positive cells with fluorescence microscopy and flow cytometry. It is also used to detect beta-galactosidase expression in live cells. Fluorescence-based assays employing FDG are also reported to be 100 to 1000-fold more sensitive than radioisotope-based ELISAs.

Protocol

SDS

Catalog	Size	Price	Quantity
14001	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FDG [Fluorescein di-beta-D-galactopyranoside] *CAS#: 17817-20-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	152.304 µL	761.522 µL	1.523 mL	7.615 mL	15.23 mL
5 mM	30.461 µL	152.304 µL	304.609 µL	1.523 mL	3.046 mL
10 mM	15.23 µL	76.152 µL	152.304 µL	761.522 µL	1.523 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	656.58 g/mol	=	mL	x	mM	=	mmol

Citations

View all 7 citations: Citation Explorer

A galactose-tethered tetraphenylethene prodrug mediated apoptosis of senescent cells for osteoporosis treatment

Authors:

Gao, Xin and Hu, Yichen and Zhang, Yingfei and Huang, Yanyan and Zhang, Guanxin and Zhang, Xiao and Zhou, Yongsheng and Zhang, Deqing

Journal:

Science Advances (2025): eadr2833

Capturing nascent extracellular vesicles by metabolic glycan labeling-assisted microfluidics

Authors:

Wu, Qiuyue and Wang, Wencheng and Zhang, Chi and You, Zhenlong and Zeng, Yinyan and Lu, Yinzhu and Zhang, Suhui and Li, Xingrui and Yang, Chaoyong and Song, Yanling

Journal:

Nature Communications (2023): 6541

Temporal Differentiation of Extracellular Vesicles by Metabolic Glycan Labeling-Assisted Microfluidics

Authors:

Wu, Qiuyue and Wang, Wencheng and Zhang, Chi and You, Zhenlong and Zeng, Yinyan and Lu, Yinzhu and Zhang, Suhui and Li, Xingrui and Yang, Chaoyong and Song, Yanling

Aptamer/antibody sandwich method for digital detection of SARS-CoV2 nucleocapsid protein

Authors:

Ge, Chenchen and Feng, Juan and Zhang, Jiaming and Hu, Kai and Wang, Dou and Zha, Ling and Hu, Xuejuan and Li, Rongsong

Journal:

Talanta (2021): 122847

Gfi1b regulates the level of Wnt/β-catenin signaling in hematopoietic stem cells and megakaryocytes

Authors:

Shooshtarizadeh, Peiman and Helness, Anne and Vadnais, Charles and Brouwer, Nelleke and Beauchemin, Hugues and Chen, Riyan and Bagci, Halil and Staal, Frank JT and Coté, Jean-Fran&ccaron;ois and Möröy, Tarik

Journal:

Nature communications (2019): 1270

References

View all 23 references: Citation Explorer

Microfluidic sequential injection analysis in a short capillary

Authors:

Du WB, Fang Q, Fang ZL.

Journal:

Anal Chem (2006): 6404

Cellular logic with orthogonal ribosomes

Authors:

Rackham O, Chin JW.

Journal:

J Am Chem Soc (2005): 17584

New transport peptides broaden the horizon of applications for peptidic pharmaceuticals

Authors:

Langedijk JP, Olijhoek T, Schut D, Autar R, Meloen RH.

Journal:

Mol Divers (2004): 101

Integration of on-column immobilized enzyme reactor in microchip electrophoresis

Authors:

Park SS, Cho SI, Kim MS, Kim YK, Kim BG.

Journal:

Electrophoresis (2003): 200

Model for focal demyelination of the spinal dorsal columns of transgenic MBP-LacZ mice by phototargeted ablation of oligodendrocytes

Authors:

V, undefined and erluit JL, Bourque JA, Peterson AC, Tetzlaff W.

Journal:

J Neurosci Res (2000): 28

Physical properties

Molecular weight	656.58
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	17817-20-8

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Enzyme Probes & Assay Kits

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Page updated on October 30, 2025