FDGlcU [Fluorescein di-beta-D-glucuronide]

FDGlcU

Fluorescein di-beta-D-glucuronide

The beta-glucuronidase (GUS) enzyme from E. coli (EC 3.2.1.31) has been well documented to provide desirable characteristics as a marker gene in transformed plants. The GUS reporter gene system has many advantages including stable expression of E. coli GUS enzyme, no interference with normal plant metabolism, and low intrinsic GUS activity in higher plants. FDGlcU is considered to be one of the most sensitive fluorogenic substrates available for detecting beta-glucuronidase. The colorless and nonfluorescent FDGlcU is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments. Glucuronidase-catalyzed hydrolysis of FDGlcU can be followed by fluorescence increase around 520 nm. Alternatively, FDGlcU can also be used to detect glucuronidase in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). FDGlcU has been used for identifying GUS-positive cells with fluorescence microscopy and flow cytometry.

Protocol

SDS

Catalog	Size	Price	Quantity
14002	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FDGlcU [Fluorescein di-beta-D-glucuronide] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	146.081 µL	730.407 µL	1.461 mL	7.304 mL	14.608 mL
5 mM	29.216 µL	146.081 µL	292.163 µL	1.461 mL	2.922 mL
10 mM	14.608 µL	73.041 µL	146.081 µL	730.407 µL	1.461 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	684.55 g/mol	=	mL	x	mM	=	mmol

References

View all 80 references: Citation Explorer

Active site tyrosine is essential for amidohydrolase but not for esterase activity of a class 2 histone deacetylase-like bacterial enzyme

Authors:

Moreth K, Riester D, Hildmann C, Hempel R, Wegener D, Schober A, Schwienhorst A.

Journal:

Biochem J. (2006)

The histone deacetylase inhibitor trichostatin a has genotoxic effects in human lymphoblasts in vitro

Authors:

Olaharski AJ, Ji Z, Woo JY, Lim S, Hubbard AE, Zhang L, Smith MT.

Journal:

Toxicol Sci (2006): 341

Histone deacetylase (HDAC) 4 involvement in both Lewy and Marinesco bodies

Authors:

Takahashi-Fujigasaki J, Fujigasaki H.

Journal:

Neuropathol Appl Neurobiol (2006): 562

Experimental therapy of malignant gliomas using the inhibitor of histone deacetylase MS-275

Authors:

Eyupoglu IY, Hahnen E, Trankle C, Savaskan NE, Siebzehnrubl FA, Buslei R, Lemke D, Wick W, Fahlbusch R, Blumcke I.

Journal:

Mol Cancer Ther (2006): 1248

Role of histone deacetylase Rpd3 in regulating rRNA gene transcription and nucleolar structure in yeast

Authors:

Oakes ML, Siddiqi I, French SL, Vu L, Sato M, Aris JP, Beyer AL, Nomura M.

Journal:

Mol Cell Biol (2006): 3889

Physical properties

Molecular weight	684.55
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Enzyme Probes & Assay Kits

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Page updated on July 12, 2023