Name: FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2*
Brand: AAT Bioquest
SKU: 11600
Availability: InStock

FDP

Fluorescein diphosphate, tetraammonium salt; CAS 217305-49-2

Upon interaction with phosphatases the colorless and non-fluorescent FDP is hydrolyzed to highly fluorescent fluorescein, which exhibits excellent spectral properties that match the optimal detection window of most fluorescence instruments that are equipped with the Argon laser excitation. Alternatively, FDP can also be used to detect phosphatases in a chromogenic mode since the enzymatic product (fluorescein) exhibits a large extinction coefficient (close to 100,000 cm-1mol-1). In some literature, FDP was considered to be one of the most sensitive fluorogenic phosphatase substrates. FDP has been widely used in various ELISA assays. Additionally it is also used to detect tyrosine phosphatases. FDP is thermally unstable, and special cautions need be excised for storing the solid sample and stock solutions.

Catalog	Size	Price	Quantity
11600	5 mg	Price
11601	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of FDP [Fluorescein diphosphate, tetraammonium salt] *CAS 217305-49-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	178.447 µL	892.236 µL	1.784 mL	8.922 mL	17.845 mL
5 mM	35.689 µL	178.447 µL	356.894 µL	1.784 mL	3.569 mL
10 mM	17.845 µL	89.224 µL	178.447 µL	892.236 µL	1.784 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	560.39 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Gain-of-function PPM1D mutations attenuate ischemic stroke

Authors:

He, Wenyan and Li, Yan and Fan, Junwan and Liu, Yang and Yuan, Meng and Cheng, Si and Huang, Xinying and Yan, Bo and Zhang, Zhuoran and Xiu, Yuwen and others,

Journal:

Cell Death \& Differentiation (2025): 1--14

Phosphatase-mimicking Zr@ PDA nanozyme with excellent dispersion stability for the detection of fructose 1, 6-diphosphate

Authors:

Huang, Yusha and Xie, Chengli and Gong, Longcheng and Wang, Min and Hu, Lianzhe and Xia, Zhining

Journal:

Talanta (2024): 126657

On-Chip Enrichment System for Digital Bioassay Based on Aqueous Two-Phase System

Authors:

Minagawa, Yoshihiro and Nakata, Shoki and Date, Motoki and Ii, Yutaro and Noji, Hiroyuki

Journal:

ACS nano (2022)

Allosteric inhibition of PPM1D serine/threonine phosphatase via an altered conformational state

Authors:

Miller, Peter G and Sathappa, Murugappan and Moroco, Jamie A and Jiang, Wei and Qian, Yue and Iqbal, Sumaiya and Guo, Qi and Giacomelli, Andrew O and Shaw, Subrata and Vernier, Camille and others,

Journal:

Nature Communications (2022): 1--16

Ratiometric fluorescent sensing and imaging of intracellular pH by an AIE-active luminogen with intrinsic phosphatase-like catalytic activity

Authors:

Dai, Ling and Mao, Weilin and Hu, Lianzhe and Song, Jiaxing and Zhang, Yan and Huang, Ting and Wang, Min

Journal:

Dyes and Pigments (2022): 110436

References

View all 33 references: Citation Explorer

TNP-8N3-ADP photoaffinity labeling of two Na,K-ATPase sequences under separate Na+ plus K+ control

Authors:

Ward DG, Taylor M, Lilley KS, Cavieres JD.

Journal:

Biochemistry (2006): 3460

Xenopus apyrase (xapy), a secreted nucleotidase that is expressed during early development

Authors:

Devader C, Webb RJ, Thomas GM, Dale L.

Journal:

Gene (2006): 135

Enzyme-nanoparticle functionalization of three-dimensional protein scaffolds

Authors:

Hill RT, Shear JB.

Journal:

Anal Chem (2006): 7022

Immunoassay for B. globigii spores as a model for detecting B. anthracis spores in finished water

Authors:

Farrell S, Halsall HB, Heineman WR.

Journal:

Analyst (2005): 489

Controlled initiation of enzymatic reactions in micrometer-sized biomimetic compartments

Authors:

Karlsson A, Sott K, Markstrom M, Davidson M, Konkoli Z, Orwar O.

Journal:

J Phys Chem B Condens Matter Mater Surf Interfaces Biophys (2005): 1609

Physical properties

Molecular weight	560.39
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	217305-49-2

Instrument settings

Fluorescence microplate reader
Excitation	490 nm
Emission	514 nm
Cutoff	500 nm
Recommended plate	Solid black

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Page updated on October 9, 2025