Ferroptosis is an iron-dependent form of regulated cell death associated with the increase in lipid peroxides. Divalent iron (Fe2+) can lead to spontaneous lipid peroxidation through the Fenton reaction. Ferroptosis is regulated by signaling pathways that control iron storage and oxidative stress. FerroBrite™ Green has been developed for detecting ferroptosis via fluorescence imaging. FerroBrite™ Green has superior photostability and responds quickly to ferroptosis. The dye is permeable to live cells. Upon induction of ferroptosis, the imbalance of Fe2+ causes the reduction in the fluorescence intensity of FerroBrite™ Green. The fluorescence of FerroBrite™ Green can be readily monitored using the common FITC filter, which is equipped in most of fluorescence instruments. FerroBrite™ Green enables the real-time tracking of ferroptosis.