Name: FISHaqua™-dUTP *1 mM in TE Buffer (pH 7.5)*
Brand: AAT Bioquest
SKU: 17049
Price: 118 USD
Availability: InStock

FISHaqua™-dUTP

1 mM in TE Buffer (pH 7.5)

FISHaqua™-dUTP is a blue-fluorescent nucleotide that incorporates efficiently into DNA probes via nick translation or random priming, providing a photostable alternative to SpectrumAqua for multicolor FISH applications.

Polymerase-friendly incorporation: Readily integrates into DNA via standard enzymatic protocols (PCR, nick translation, random priming, reverse transcription), streamlining probe preparation for FISH and microarray assays
SpectrumAqua™-matched fluorescence: Emits bright blue signal optimized for SpectrumAqua™ filter sets, ensuring clear detection without additional optical adjustments
pH-independent, photostable signal: Delivers high-intensity fluorescence that resists photobleaching and remains stable across physiological pH ranges for reliable imaging sessions

Datasheet

SDS

Catalog	Size	Price	Quantity
17049	25 nmoles	Price

Overview

FISHaqua™-dUTP can be readily incorporated into DNAs in the presence of a polymerase. It is an excellent substrate for labeling DNAs for the Fluorescence in situ hybridization (FISH) (FISH) applications. FISHaqua™-dUTP has the same spectral properties well compatible with the filter set of SpectrumAqua™. It is used as a blue fluorescence color with SpectrumAqua™ filter set. It provides a bright signal that has high photostability and is not affected by pH. The dye-modified deoxyuridine 5'-triphosphates are one of the most common methods to produce dye-labelled DNA via the conventional enzymatic incorporation methods such as reverse transcription, nick translation, random primed labeling, or PCR. This enzymatic fluorescence labeling method is widely used for both FISH probes and microarray-based experiments. SpectrumAqua™ is the trademark of Vysis.

References

View all 50 references: Citation Explorer

RNAscope Multiplex FISH Signal Assessment in FFPE and Fresh Frozen Tissues: The Effect of Archival Duration on RNA Expression.

Authors:

Sari, Ariestya Indah Permata and Copeland, Katherine and Nuwongsri, Pattarin and Pipatsakulroj, Wiriya and Jinawath, Artit and Israsena, Nipan and Lertsittichai, Panuwat and Chirappapha, Prakasit and Shiao, Meng-Shin and Jinawath, Natini

Journal:

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society (2025): 9-28

Development of a quadruple Taqman probe-based real-time fluorescent quantitative PCR for the detection of bacterial pathogens in a marine fish.

Authors:

Dong, Yuchen and Hu, Liping and Zhang, Jianbai and Zhou, Dandan and Zhang, Binzhe and Li, Xuepeng and Zhang, Jian

Journal:

Microbial pathogenesis (2025): 107459

SABER-FISH in Hydractinia.

Authors:

Salinas-Saavedra, Miguel

Journal:

Methods in molecular biology (Clifton, N.J.) (2024): 77-85

SnapFISH-IMPUTE: an imputation method for multiplexed DNA FISH data.

Authors:

Yu, Hongyu and Wu, Daiqing and Mishra, Shreya and Shen, Guning and Sun, Huaigu and Hu, Ming and Li, Yun

Journal:

Communications biology (2024): 834

Designing Oligonucleotide-Based FISH Probe Sets with PaintSHOP.

Authors:

Perez, Monika W and Camplisson, Conor K and Beliveau, Brian J

Journal:

Methods in molecular biology (Clifton, N.J.) (2024): 177-189

Physical properties

Molecular weight	959.15
Solvent	Water

Spectral properties

Excitation (nm)	432
Emission (nm)	479

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 8, 2026