Name: FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK
Brand: AAT Bioquest
SKU: 13417
Availability: InStock

FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK

Activation of caspases plays a central role in apoptosis. FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK provides a convenient means for sensitive detection of activated caspase-3 in living cells. FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells. Based on our in-house studies FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK gave the highest signal/background ratio compared to other DEVD-FMK probes we tested. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

Protocol

COA

Catalog	Size	Price	Quantity
13417	100 ug	Price
13418	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.426 µL	482.128 µL	964.255 µL	4.821 mL	9.643 mL
5 mM	19.285 µL	96.426 µL	192.851 µL	964.255 µL	1.929 mL
10 mM	9.643 µL	48.213 µL	96.426 µL	482.128 µL	964.255 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1037.07 g/mol	=	mL	x	mM	=	mmol

References

View all 4 references: Citation Explorer

Antitumor mechanism of 3-bromopropionylamino benzoylurea on leukemia and lymphoma

Authors:

Li JN, Song DQ, Jiang JD.

Journal:

Yao Xue Xue Bao (2004): 491

Activation of caspase-like activity and poly (ADP-ribose) polymerase degradation during sporulation in Aspergillus nidulans

Authors:

Thrane C, Kaufmann U, Stummann BM, Olsson S.

Journal:

Fungal Genet Biol (2004): 361

Caspase-dependent and independent cell death in rat hepatoma 5123tc cells

Authors:

P, undefined and ey S, Smith B, Walker PR, Sikorska M.

Journal:

Apoptosis (2000): 265

C-myc antisense oligodeoxynucleotides can induce apoptosis and down-regulate Fas expression in rheumatoid synoviocytes

Authors:

Hashiramoto A, Sano H, Maekawa T, Kawahito Y, Kimura S, Kusaka Y, Wilder RL, Kato H, Kondo M, Nakajima H.

Journal:

Arthritis Rheum (1999): 954

Physical properties

Molecular weight	1037.07
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025