FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK

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Physical properties

Molecular weight	1037.07
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

1037.07

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.92

Activation of caspases plays a central role in apoptosis. FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK provides a convenient means for sensitive detection of activated caspase-3 in living cells. FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-3 in apoptotic cells. Based on our in-house studies FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK gave the highest signal/background ratio compared to other DEVD-FMK probes we tested. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.426 µL	482.128 µL	964.255 µL	4.821 mL	9.643 mL
5 mM	19.285 µL	96.426 µL	192.851 µL	964.255 µL	1.929 mL
10 mM	9.643 µL	48.213 µL	96.426 µL	482.128 µL	964.255 µL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Images

Figure 1. Fluorometric detection of active caspases 3/7 using FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK (Cat# 13418) in Jurkat cells. The cells were treated with 1 μM staurosporine for 4 hours (Green) while untreated cells were used as a control (Red). Control or treated Cells were incubated with FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK for 1 hour at 37 °C, and then washed once after stain. Fluorescent intensity was measured with NovoCyte™ 3000 Flow Cytometer FITC channel.

Figure 2. Chemical structure for FITC-C6-D(OMe)E(OMe)VD(OMe)-FMK

References

View all 4 references: Citation Explorer

Activation of caspase-like activity and poly (ADP-ribose) polymerase degradation during sporulation in Aspergillus nidulans
Authors: Thrane C, Kaufmann U, Stummann BM, Olsson S.
Journal: Fungal Genet Biol (2004): 361

Antitumor mechanism of 3-bromopropionylamino benzoylurea on leukemia and lymphoma
Authors: Li JN, Song DQ, Jiang JD.
Journal: Yao Xue Xue Bao (2004): 491

Caspase-dependent and independent cell death in rat hepatoma 5123tc cells
Authors: P, undefined and ey S, Smith B, Walker PR, Sikorska M.
Journal: Apoptosis (2000): 265

C-myc antisense oligodeoxynucleotides can induce apoptosis and down-regulate Fas expression in rheumatoid synoviocytes
Authors: Hashiramoto A, Sano H, Maekawa T, Kawahito Y, Kimura S, Kusaka Y, Wilder RL, Kato H, Kondo M, Nakajima H.
Journal: Arthritis Rheum (1999): 954