Name: FITC-C6-LEHD-FMK
Brand: AAT Bioquest
SKU: 13407
Availability: InStock

FITC-C6-LEHD-FMK

Activation of caspases plays a central role in apoptosis. FITC-C6-LEHD-FMK provides a convenient means for sensitive detection of activated caspase-9 in living cells. FITC-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase-9 in apoptotic cells. The FITC label allows for direct detection of activated caspases in apoptotic cells by fluorescence microscopy, flow cytometry, or fluorescence plate reader.

Protocol

COA

Catalog	Size	Price	Quantity
13407	1 mg	Price
13409	100 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FITC-C6-LEHD-FMK to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	96.987 µL	484.933 µL	969.866 µL	4.849 mL	9.699 mL
5 mM	19.397 µL	96.987 µL	193.973 µL	969.866 µL	1.94 mL
10 mM	9.699 µL	48.493 µL	96.987 µL	484.933 µL	969.866 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1031.07 g/mol	=	mL	x	mM	=	mmol

References

View all 9 references: Citation Explorer

Endoplasmic reticulum stress and trophic factor withdrawal activate distinct signaling cascades that induce glycogen synthase kinase-3 beta and a caspase-9-dependent apoptosis in cerebellar granule neurons

Authors:

Brewster JL, Linseman DA, Bouchard RJ, Loucks FA, Precht TA, Esch EA, Heidenreich KA.

Journal:

Mol Cell Neurosci (2006): 242

Enhanced 15-HPETE production during oxidant stress induces apoptosis of endothelial cells

Authors:

Sordillo LM, Weaver JA, Cao YZ, Corl C, Sylte MJ, Mullarky IK.

Journal:

Prostaglandins Other Lipid Mediat (2005): 19

P2X7 receptor-mediated apoptosis of human cervical epithelial cells

Authors:

Wang Q, Wang L, Feng YH, Li X, Zeng R, Gorodeski GI.

Journal:

Am J Physiol Cell Physiol (2004): C1349

Induction of apoptosis by apicidin, a histone deacetylase inhibitor, via the activation of mitochondria-dependent caspase cascades in human Bcr-Abl-positive leukemia cells

Authors:

Cheong JW, Chong SY, Kim JY, Eom JI, Jeung HK, Maeng HY, Lee ST, Min YH.

Journal:

Clin Cancer Res (2003): 5018

Caspase activation during Haemophilus somnus lipooligosaccharide-mediated apoptosis of bovine endothelial cells

Authors:

Sylte MJ, Leite FP, Kuckleburg CJ, Inzana TJ, Czuprynski CJ.

Journal:

Microb Pathog (2003): 285

Physical properties

Molecular weight	1031.07
Solvent	DMSO

Spectral properties

Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 12, 2025