FITC–dextran conjugate (average MW = ~150K)

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Physical properties

Molecular weight	~150000
Solvent	Water

Spectral properties

Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

~150000

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

73000

Excitation (nm)

491

Emission (nm)

516

Quantum yield

0.92

FITC-labeled dextran is used in cardiovascular, microcirculation, perfusion, cell monolayer and cell membrane permeability research as fluorescent flux tracer compound that supports the measurement of processes such as blood flow, membrane damage, vascular drainage and renal elimination. It is also used in microcirculation and cell permeability research utilizing microfluorimetry, and possible in perfusion studies in animals. FITC-dextran has also been used to study plant cell wall porosity and capillary permeability. Plasma proteins have been shown not to bind to FITC-dextran. Fluorescein-dextran of150 kDa may be used to help assess the integrity of the blood brain barrier (BBB) and to perform fluorescence microlymphography. It may also be used as a microcirculation vascular flux tracer.

Spectrum

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Spectral properties

Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	73000
Excitation (nm)	491
Emission (nm)	516
Quantum yield	0.92

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
FITC–dextran conjugate (average MW = ~20K)	491	516	73000	0.92	0.35
FITC–dextran conjugate (average MW = ~70K)	491	516	73000	0.92	0.35
TRITC–dextran conjugate (average MW = ~150K)	544	570	100000	-	0.34

Images

Figure 1. Chemical structure for FITC–dextran conjugate (average MW = ~150K).

Citations

View all 43 citations: Citation Explorer

Lymphatic Reconnection and Restoration of Lymphatic Flow by Nonvascularized Lymph Node Transplantation: Real-Time Fluorescence Imaging Using Indocyanine Green and Fluorescein Isothiocyanate-Dextran
Authors: Maeda, T., Yamamoto, Y., Iwasaki, D., Hayashi, T., Funayama, E., Oyama, A., Murao, N., Furukawa, H.
Journal: Lymphat Res Biol (2018): 165-173

Optimizing Fluorescein Isothiocyanate Dextran Measurement As a Biomarker in a 24-h Feed Restriction Model to Induce Gut Permeability in Broiler Chickens
Authors: Baxter, M. F. A., Merino-Guzman, R., Latorre, J. D., Mahaffey, B. D., Yang, Y., Teague, K. D., Graham, L. E., Wolfenden, A. D., Hern and ez-Velasco, X., Bielke, L. R., Hargis, B. M., Tellez, G.
Journal: Front Vet Sci (2017): 56

Fluorescein Isothiocyanate (FITC)-Dextran Extravasation as a Measure of Blood-Brain Barrier Permeability
Authors: Natarajan, R., Northrop, N., Yamamoto, B.
Journal: Curr Protoc Neurosci (2017): 9 58 1-9 58 15

Adenosine receptor agonist NECA increases cerebral extravasation of fluorescein and low molecular weight dextran independent of blood-brain barrier modulation
Authors: Cheng, C. C., Yang, Y. L., Liao, K. H., Lai, T. W.
Journal: Sci Rep (2016): 23882

Analysis of hair follicle penetration of lidocaine and fluorescein isothiocyanate-dextran 4 kDa using hair follicle-plugging method
Authors: Horita, D., Yoshimoto, M., Todo, H., Sugibayashi, K.
Journal: Drug Dev Ind Pharm (2014): 345-51

Fluorescein-dextran sequestration in the reproductive tract of the migratory grasshopper Melanoplus sanguinipes (Orthoptera, Acridiidae)
Authors: Jones, N., Taub-Montemayor, T., Rankin, M. A.
Journal: Micron (2013): 80-4

Evaluation of changes in hepatic disposition of phenolsulfonphthalein, indocyanine green and fluorescein isothiocyanate-dextran at low temperatures using a rat liver perfusion system
Authors: Miyamoto, H., Miyake, H., Yoshikawa, N., Hirata, H., Ohwaki, Y., Fumoto, S., Sasaki, H., Nakamura, J., Nishida, K.
Journal: J Pharm Pharmacol (2012): 848-54

Effects of vitreous liquefaction on the intravitreal distribution of sodium fluorescein, fluorescein dextran, and fluorescent microparticles
Authors: Tan, L. E., Orilla, W., Hughes, P. M., Tsai, S., Burke, J. A., Wilson, C. G.
Journal: Invest Ophthalmol Vis Sci (2011): 1111-8

Lineage labeling of zebrafish cells with laser uncagable fluorescein dextran
Authors: Clanton, J. A., Shestopalov, I., Chen, J. K., Gamse, J. T.
Journal: J Vis Exp (2011): se name="21700.enl" path="C:\Users\aatbi\Drop

A method for assessment of blood volume parameters in pregnant sheep using fluorescein-labelled dextran
Authors: Rumball, C. W., Van Zijl, P., Rutl and , M. D., Bloomfield, F. H., Harding, J. E.
Journal: Placenta (2008): 15-9