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FITC-Palmitate Conjugate

Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde.  Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde.  Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
Chemical structure for FITC-Palmitate Conjugate.
Ordering information
Price ()
Catalog Number22192
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
SolventDMSO
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.275
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Absorbance (nm)
487
Correction Factor (260 nm)
0.32
Correction Factor (280 nm)
0.275
Extinction coefficient (cm -1 M -1)
800001
Excitation (nm)
498
Emission (nm)
517
Quantum yield
0.79001, 0.952
FITC-Palmitate Conjugate is a highly hydrophobic fatty acid lipid that is tagged with fluorescein. Palmitic acid is a saturated C16 fatty acid that can interact well with the hydrophobic cell membranes or lysosome membranes. Fluorescein-modified palmitic acid has strong green fluorescence that can well be imaged with the standard FITC filter set. AAT Bioquest also offer other dye-labeled lipids, such as phospholipids, sphingolipids, sterols, glycerolipids, and others.

Platform


Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.275
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952

Product family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
FITC-streptavidin conjugate491516730000.920.254

References


View all 37 references: Citation Explorer
Planarian toxicity fluorescent assay: A rapid and cheap pre-screening tool for potential skin irritants.
Authors: Shah, Syed Ibrahim and Williams, Adrian C and Lau, Wing Man and Khutoryanskiy, Vitaliy V
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2020): 105004
MIP-1α Expression Induced by Co-Stimulation of Human Monocytic Cells with Palmitate and TNF-α Involves the TLR4-IRF3 Pathway and Is Amplified by Oxidative Stress.
Authors: Sindhu, Sardar and Akhter, Nadeem and Wilson, Ajit and Thomas, Reeby and Arefanian, Hossein and Al Madhoun, Ashraf and Al-Mulla, Fahd and Ahmad, Rasheed
Journal: Cells (2020)
Fluorescent labelling of membrane fatty acid transporter CD36 (SR-B2) in the extracellular loop.
Authors: Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert
Journal: PloS one (2019): e0210704
Palmitate up-regulates laminin expression via ROS/integrin αvβ3 pathway in HLSECs.
Authors: Zhang, Qi and Jiang, Luxia and Yu, Jing and Tian, Limin and Guo, Tiankang and Di, Baoshan and Quan, Jinxing and Feng, Jing and Liu, Jing
Journal: Oncotarget (2019): 4083-4090
Mucus permeating self-emulsifying drug delivery systems (SEDDS): About the impact of mucolytic enzymes.
Authors: Efiana, Nuri Ari and Phan, Thi Nhu Quynh and Wicaksono, Arko Jatmiko and Bernkop-Schnürch, Andreas
Journal: Colloids and surfaces. B, Biointerfaces (2018): 228-235
Efficacy and safety of retinol palmitate ophthalmic solution in the treatment of dry eye: a Japanese Phase II clinical trial.
Authors: Toshida, Hiroshi and Funaki, Toshinari and Ono, Koichi and Tabuchi, Nobuhito and Watanabe, Sota and Seki, Tamotsu and Otake, Hiroshi and Kato, Takuji and Ebihara, Nobuyuki and Murakami, Akira
Journal: Drug design, development and therapy (2017): 1871-1879
Neutral Ceramidase Secreted Via Exosome Protects Against Palmitate-Induced Apoptosis in INS-1 Cells.
Authors: Tang, S and Luo, F and Feng, Y M and Wei, X and Miao, H and Lu, Y B and Tang, Y and Ding, D F and Jin, J F and Zhu, Q
Journal: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Ass (2017): 130-135
Lack of Impact of High Dietary Vitamin A on T Helper 2-Dependent Contact Hypersensitivity to Fluorescein Isothiocyanate in Mice.
Authors: Kobayashi, Chie and Kurohane, Kohta and Imai, Yasuyuki
Journal: Biological & pharmaceutical bulletin (2015): 1827-30
Inhibition of de novo Palmitate Synthesis by Fatty Acid Synthase Induces Apoptosis in Tumor Cells by Remodeling Cell Membranes, Inhibiting Signaling Pathways, and Reprogramming Gene Expression.
Authors: Ventura, Richard and Mordec, Kasia and Waszczuk, Joanna and Wang, Zhaoti and Lai, Julie and Fridlib, Marina and Buckley, Douglas and Kemble, George and Heuer, Timothy S
Journal: EBioMedicine (2015): 808-24
Sucrose esters increase drug penetration, but do not inhibit p-glycoprotein in caco-2 intestinal epithelial cells.
Authors: Kiss, Lóránd and Hellinger, Éva and Pilbat, Ana-Maria and Kittel, Ágnes and Török, Zsolt and Füredi, András and Szakács, Gergely and Veszelka, Szilvia and Sipos, Péter and Ózsvári, Béla and Puskás, László G and Vastag, Monika and Szabó-Révész, Piroska and Deli, Mária A
Journal: Journal of pharmaceutical sciences (2014): 3107-19