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FITC-Palmitate Conjugate
FITC-Palmitate Conjugate is a highly hydrophobic fatty acid lipid that is tagged with fluorescein. Palmitic acid is a saturated C16 fatty acid that can interact well with the hydrophobic cell membranes or lysosome membranes. Fluorescein-modified palmitic acid has strong green fluorescence that can well be imaged with the standard FITC filter set. AAT Bioquest also offer other dye-labeled lipids, such as phospholipids, sphingolipids, sterols, glycerolipids, and others.
Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
221925 mg
Price
 
Physical properties
Molecular weight848.11
SolventDMSO
Spectral properties
Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
, 
0.95
2
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 25, 2025