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FITC-Palmitate Conjugate

Documents
pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
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Application notes
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FITC-Palmitate Conjugate is a highly hydrophobic fatty acid lipid that is tagged with fluorescein. Palmitic acid is a saturated C16 fatty acid that can interact well with the hydrophobic cell membranes or lysosome membranes. Fluorescein-modified palmitic acid has strong green fluorescence that can well be imaged with the standard FITC filter set. AAT Bioquest also offer other dye-labeled lipids, such as phospholipids, sphingolipids, sterols, glycerolipids, and others.
Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
Gallery Image 2
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Catalog Number22192
Quantity
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Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
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Physical properties
Molecular weight848.11
SolventDMSO
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501
Platform

Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall, clear bottom
Spectrum
Product family
NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
FITC-streptavidin conjugate491516730000.920.35
References
View all 37 references: Citation Explorer
Planarian toxicity fluorescent assay: A rapid and cheap pre-screening tool for potential skin irritants.
Authors: Shah, Syed Ibrahim and Williams, Adrian C and Lau, Wing Man and Khutoryanskiy, Vitaliy V
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2020): 105004
MIP-1α Expression Induced by Co-Stimulation of Human Monocytic Cells with Palmitate and TNF-α Involves the TLR4-IRF3 Pathway and Is Amplified by Oxidative Stress.
Authors: Sindhu, Sardar and Akhter, Nadeem and Wilson, Ajit and Thomas, Reeby and Arefanian, Hossein and Al Madhoun, Ashraf and Al-Mulla, Fahd and Ahmad, Rasheed
Journal: Cells (2020)
Fluorescent labelling of membrane fatty acid transporter CD36 (SR-B2) in the extracellular loop.
Authors: Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert
Journal: PloS one (2019): e0210704
Palmitate up-regulates laminin expression via ROS/integrin αvβ3 pathway in HLSECs.
Authors: Zhang, Qi and Jiang, Luxia and Yu, Jing and Tian, Limin and Guo, Tiankang and Di, Baoshan and Quan, Jinxing and Feng, Jing and Liu, Jing
Journal: Oncotarget (2019): 4083-4090
Mucus permeating self-emulsifying drug delivery systems (SEDDS): About the impact of mucolytic enzymes.
Authors: Efiana, Nuri Ari and Phan, Thi Nhu Quynh and Wicaksono, Arko Jatmiko and Bernkop-Schnürch, Andreas
Journal: Colloids and surfaces. B, Biointerfaces (2018): 228-235
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