FITC-Palmitate Conjugate

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Physical properties

Molecular weight	848.11
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Platform

Fluorescence microscope

Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of FITC-Palmitate Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	117.909 µL	589.546 µL	1.179 mL	5.895 mL	11.791 mL
5 mM	23.582 µL	117.909 µL	235.818 µL	1.179 mL	2.358 mL
10 mM	11.791 µL	58.955 µL	117.909 µL	589.546 µL	1.179 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)
FITC-streptavidin conjugate	491	516	73000	0.92	0.35

Images

Figure 1. Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.

Figure 2. Chemical structure for FITC-Palmitate Conjugate.

References

View all 37 references: Citation Explorer

Planarian toxicity fluorescent assay: A rapid and cheap pre-screening tool for potential skin irritants.
Authors: Shah, Syed Ibrahim and Williams, Adrian C and Lau, Wing Man and Khutoryanskiy, Vitaliy V
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2020): 105004

MIP-1α Expression Induced by Co-Stimulation of Human Monocytic Cells with Palmitate and TNF-α Involves the TLR4-IRF3 Pathway and Is Amplified by Oxidative Stress.
Authors: Sindhu, Sardar and Akhter, Nadeem and Wilson, Ajit and Thomas, Reeby and Arefanian, Hossein and Al Madhoun, Ashraf and Al-Mulla, Fahd and Ahmad, Rasheed
Journal: Cells (2020)

Fluorescent labelling of membrane fatty acid transporter CD36 (SR-B2) in the extracellular loop.
Authors: Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert
Journal: PloS one (2019): e0210704

Palmitate up-regulates laminin expression via ROS/integrin αvβ3 pathway in HLSECs.
Authors: Zhang, Qi and Jiang, Luxia and Yu, Jing and Tian, Limin and Guo, Tiankang and Di, Baoshan and Quan, Jinxing and Feng, Jing and Liu, Jing
Journal: Oncotarget (2019): 4083-4090

Mucus permeating self-emulsifying drug delivery systems (SEDDS): About the impact of mucolytic enzymes.
Authors: Efiana, Nuri Ari and Phan, Thi Nhu Quynh and Wicaksono, Arko Jatmiko and Bernkop-Schnürch, Andreas
Journal: Colloids and surfaces. B, Biointerfaces (2018): 228-235

Efficacy and safety of retinol palmitate ophthalmic solution in the treatment of dry eye: a Japanese Phase II clinical trial.
Authors: Toshida, Hiroshi and Funaki, Toshinari and Ono, Koichi and Tabuchi, Nobuhito and Watanabe, Sota and Seki, Tamotsu and Otake, Hiroshi and Kato, Takuji and Ebihara, Nobuyuki and Murakami, Akira
Journal: Drug design, development and therapy (2017): 1871-1879

Neutral Ceramidase Secreted Via Exosome Protects Against Palmitate-Induced Apoptosis in INS-1 Cells.
Authors: Tang, S and Luo, F and Feng, Y M and Wei, X and Miao, H and Lu, Y B and Tang, Y and Ding, D F and Jin, J F and Zhu, Q
Journal: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Ass (2017): 130-135

Lack of Impact of High Dietary Vitamin A on T Helper 2-Dependent Contact Hypersensitivity to Fluorescein Isothiocyanate in Mice.
Authors: Kobayashi, Chie and Kurohane, Kohta and Imai, Yasuyuki
Journal: Biological & pharmaceutical bulletin (2015): 1827-30

Inhibition of de novo Palmitate Synthesis by Fatty Acid Synthase Induces Apoptosis in Tumor Cells by Remodeling Cell Membranes, Inhibiting Signaling Pathways, and Reprogramming Gene Expression.
Authors: Ventura, Richard and Mordec, Kasia and Waszczuk, Joanna and Wang, Zhaoti and Lai, Julie and Fridlib, Marina and Buckley, Douglas and Kemble, George and Heuer, Timothy S
Journal: EBioMedicine (2015): 808-24

Sucrose esters increase drug penetration, but do not inhibit p-glycoprotein in caco-2 intestinal epithelial cells.
Authors: Kiss, Lóránd and Hellinger, Éva and Pilbat, Ana-Maria and Kittel, Ágnes and Török, Zsolt and Füredi, András and Szakács, Gergely and Veszelka, Szilvia and Sipos, Péter and Ózsvári, Béla and Puskás, László G and Vastag, Monika and Szabó-Révész, Piroska and Deli, Mária A
Journal: Journal of pharmaceutical sciences (2014): 3107-19