logo
AAT Bioquest

 
search
logo
AAT Bioquest
Image Gallery
Contact us
Company
Telephone:1-408-733-1055
Fax:1-408-733-1304
Hours:Monday to Friday
8:30 - 17:30 PST (GMT-8)
Location:5775 W Las Positas Blvd.
Pleasanton, CA, 94588
USA

Email us
Sales:sales@aatbio.com
Technical Support:support@aatbio.com
Website:websupport@aatbio.com
General Inquiries:info@aatbio.com
Order info
United States of America
Telephone:1-408-733-1055
Fax:1-408-733-1304
Email:sales@aatbio.com
Purchase Order:sales@aatbio.com

International
Click here to see all available distributors
 
search
calculatorCalculator

FITC-Palmitate Conjugate

FITC-Palmitate Conjugate is a highly hydrophobic fatty acid lipid that is tagged with fluorescein. Palmitic acid is a saturated C16 fatty acid that can interact well with the hydrophobic cell membranes or lysosome membranes. Fluorescein-modified palmitic acid has strong green fluorescence that can well be imaged with the standard FITC filter set. AAT Bioquest also offer other dye-labeled lipids, such as phospholipids, sphingolipids, sterols, glycerolipids, and others.

Example protocol

PREPARATION OF STOCK SOLUTIONS

Unless otherwise noted, all unused stock solutions should be divided into single-use aliquots and stored at -20 °C after preparation. Avoid repeated freeze-thaw cycles

FITC-Palmitate Conjugate stock solution

Prepare a 5 to 10 mM stock solution by dissolving an appropriate amount of FITC-Palmitate Conjugate in DMSO.

Note: For example, to prepare a 5 mM DMSO stock solution dissolve 1 mg of the FITC-Pamitate conjugate in 235 µL of DMSO.

PREPARATION OF WORKING SOLUTION

FITC-Palmitate Conjugate working solution

Prepare a 5 to 10 µM FITC-Palmitate conjugate working solution by diluting the stock solution in HHBS.

Note: Prepare a fresh working solution before use.

SAMPLE EXPERIMENTAL PROTOCOL

The following protocol can be used as a guideline. If needed, optimize the protocol to achieve the desired results.

  1. Culture cells as desired.

  2. Remove cell culture medium.

    Note: Cell culture medium must be removed since it may interfere with the conjugate staining.

  3. Add 100 µL of FITC-Palmitate Conjugate working solution per well.

  4. Incubate the cells in the dark for 30 minutes at room temperature.

  5. Remove the FITC-Palmitate Conjugate working solution and wash cells twice with HHBS.

  6. Add HHBS buffer to the cells and observe the cells using a fluorescence microscope with a FITC filter set.

  7. Optional: The cells can be fixed after staining.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
FITC-streptavidin conjugate491516730000.920.35

References

View all 37 references: Citation Explorer
Planarian toxicity fluorescent assay: A rapid and cheap pre-screening tool for potential skin irritants.
Authors: Shah, Syed Ibrahim and Williams, Adrian C and Lau, Wing Man and Khutoryanskiy, Vitaliy V
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2020): 105004
MIP-1α Expression Induced by Co-Stimulation of Human Monocytic Cells with Palmitate and TNF-α Involves the TLR4-IRF3 Pathway and Is Amplified by Oxidative Stress.
Authors: Sindhu, Sardar and Akhter, Nadeem and Wilson, Ajit and Thomas, Reeby and Arefanian, Hossein and Al Madhoun, Ashraf and Al-Mulla, Fahd and Ahmad, Rasheed
Journal: Cells (2020)
Fluorescent labelling of membrane fatty acid transporter CD36 (SR-B2) in the extracellular loop.
Authors: Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert
Journal: PloS one (2019): e0210704
Palmitate up-regulates laminin expression via ROS/integrin αvβ3 pathway in HLSECs.
Authors: Zhang, Qi and Jiang, Luxia and Yu, Jing and Tian, Limin and Guo, Tiankang and Di, Baoshan and Quan, Jinxing and Feng, Jing and Liu, Jing
Journal: Oncotarget (2019): 4083-4090
Mucus permeating self-emulsifying drug delivery systems (SEDDS): About the impact of mucolytic enzymes.
Authors: Efiana, Nuri Ari and Phan, Thi Nhu Quynh and Wicaksono, Arko Jatmiko and Bernkop-Schnürch, Andreas
Journal: Colloids and surfaces. B, Biointerfaces (2018): 228-235
Page updated on August 21, 2025

Ordering information

Price
Unit size
Catalog Number22192
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

848.11

Solvent

DMSO

Spectral properties

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm -1 M -1)

800001

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.79001, 0.952

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

Platform

Fluorescence microscope

ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall, clear bottom
Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
Gallery Image 2

Documents

pdfSafety data sheet
pdfProduct protocol
pdfCertificate of analysis
Fluoresceins