FITC-Palmitate Conjugate
Ordering information
Price | |
Catalog Number | |
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 848.11 |
Solvent | DMSO |
Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | ![]() ![]() |
See also: Fluoresceins
Molecular weight 848.11 | Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
FITC-Palmitate Conjugate is a highly hydrophobic fatty acid lipid that is tagged with fluorescein. Palmitic acid is a saturated C16 fatty acid that can interact well with the hydrophobic cell membranes or lysosome membranes. Fluorescein-modified palmitic acid has strong green fluorescence that can well be imaged with the standard FITC filter set. AAT Bioquest also offer other dye-labeled lipids, such as phospholipids, sphingolipids, sterols, glycerolipids, and others.
Platform
Fluorescence microscope
Excitation | FITC filter set |
Emission | FITC filter set |
Recommended plate | Black wall/clear bottom |
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of FITC-Palmitate Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 117.909 µL | 589.546 µL | 1.179 mL | 5.895 mL | 11.791 mL |
5 mM | 23.582 µL | 117.909 µL | 235.818 µL | 1.179 mL | 2.358 mL |
10 mM | 11.791 µL | 58.955 µL | 117.909 µL | 589.546 µL | 1.179 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
/ | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer


Spectral properties
Absorbance (nm) | 487 |
Correction Factor (260 nm) | 0.32 |
Correction Factor (280 nm) | 0.35 |
Extinction coefficient (cm -1 M -1) | 800001 |
Excitation (nm) | 498 |
Emission (nm) | 517 |
Quantum yield | 0.79001, 0.952 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (280 nm) |
FITC-streptavidin conjugate | 491 | 516 | 73000 | 0.92 | 0.35 |
Images

Figure 1. Image of Hela cells stained with FITC-Palmitate Conjugate and then fixed with 4% formaldehyde. Live Hela cells were cultured in a 96-well plate overnight, and the medium was removed before staining. 100 µL of the FITC-Palmitate conjugate (10 µM) in HH buffer was added to each well. Cells were stained for 30 minutes at room temperature, washed twice with HH buffer, and then fixed with 4% formaldehyde. The cells were imaged with a FITC filter before and after fixation.
References
View all 37 references: Citation Explorer
Planarian toxicity fluorescent assay: A rapid and cheap pre-screening tool for potential skin irritants.
Authors: Shah, Syed Ibrahim and Williams, Adrian C and Lau, Wing Man and Khutoryanskiy, Vitaliy V
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2020): 105004
Authors: Shah, Syed Ibrahim and Williams, Adrian C and Lau, Wing Man and Khutoryanskiy, Vitaliy V
Journal: Toxicology in vitro : an international journal published in association with BIBRA (2020): 105004
MIP-1α Expression Induced by Co-Stimulation of Human Monocytic Cells with Palmitate and TNF-α Involves the TLR4-IRF3 Pathway and Is Amplified by Oxidative Stress.
Authors: Sindhu, Sardar and Akhter, Nadeem and Wilson, Ajit and Thomas, Reeby and Arefanian, Hossein and Al Madhoun, Ashraf and Al-Mulla, Fahd and Ahmad, Rasheed
Journal: Cells (2020)
Authors: Sindhu, Sardar and Akhter, Nadeem and Wilson, Ajit and Thomas, Reeby and Arefanian, Hossein and Al Madhoun, Ashraf and Al-Mulla, Fahd and Ahmad, Rasheed
Journal: Cells (2020)
Fluorescent labelling of membrane fatty acid transporter CD36 (SR-B2) in the extracellular loop.
Authors: Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert
Journal: PloS one (2019): e0210704
Authors: Liu, Yilin and Rodriguez-Calvo, Ricardo and Wang, Shujin and Zhu, Xiaoqing and Broers, Jos L V and Glatz, Jan F C and Luiken, Joost J F P and Neumann, Dietbert
Journal: PloS one (2019): e0210704
Palmitate up-regulates laminin expression via ROS/integrin αvβ3 pathway in HLSECs.
Authors: Zhang, Qi and Jiang, Luxia and Yu, Jing and Tian, Limin and Guo, Tiankang and Di, Baoshan and Quan, Jinxing and Feng, Jing and Liu, Jing
Journal: Oncotarget (2019): 4083-4090
Authors: Zhang, Qi and Jiang, Luxia and Yu, Jing and Tian, Limin and Guo, Tiankang and Di, Baoshan and Quan, Jinxing and Feng, Jing and Liu, Jing
Journal: Oncotarget (2019): 4083-4090
Mucus permeating self-emulsifying drug delivery systems (SEDDS): About the impact of mucolytic enzymes.
Authors: Efiana, Nuri Ari and Phan, Thi Nhu Quynh and Wicaksono, Arko Jatmiko and Bernkop-Schnürch, Andreas
Journal: Colloids and surfaces. B, Biointerfaces (2018): 228-235
Authors: Efiana, Nuri Ari and Phan, Thi Nhu Quynh and Wicaksono, Arko Jatmiko and Bernkop-Schnürch, Andreas
Journal: Colloids and surfaces. B, Biointerfaces (2018): 228-235
Efficacy and safety of retinol palmitate ophthalmic solution in the treatment of dry eye: a Japanese Phase II clinical trial.
Authors: Toshida, Hiroshi and Funaki, Toshinari and Ono, Koichi and Tabuchi, Nobuhito and Watanabe, Sota and Seki, Tamotsu and Otake, Hiroshi and Kato, Takuji and Ebihara, Nobuyuki and Murakami, Akira
Journal: Drug design, development and therapy (2017): 1871-1879
Authors: Toshida, Hiroshi and Funaki, Toshinari and Ono, Koichi and Tabuchi, Nobuhito and Watanabe, Sota and Seki, Tamotsu and Otake, Hiroshi and Kato, Takuji and Ebihara, Nobuyuki and Murakami, Akira
Journal: Drug design, development and therapy (2017): 1871-1879
Neutral Ceramidase Secreted Via Exosome Protects Against Palmitate-Induced Apoptosis in INS-1 Cells.
Authors: Tang, S and Luo, F and Feng, Y M and Wei, X and Miao, H and Lu, Y B and Tang, Y and Ding, D F and Jin, J F and Zhu, Q
Journal: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Ass (2017): 130-135
Authors: Tang, S and Luo, F and Feng, Y M and Wei, X and Miao, H and Lu, Y B and Tang, Y and Ding, D F and Jin, J F and Zhu, Q
Journal: Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Ass (2017): 130-135
Lack of Impact of High Dietary Vitamin A on T Helper 2-Dependent Contact Hypersensitivity to Fluorescein Isothiocyanate in Mice.
Authors: Kobayashi, Chie and Kurohane, Kohta and Imai, Yasuyuki
Journal: Biological & pharmaceutical bulletin (2015): 1827-30
Authors: Kobayashi, Chie and Kurohane, Kohta and Imai, Yasuyuki
Journal: Biological & pharmaceutical bulletin (2015): 1827-30
Inhibition of de novo Palmitate Synthesis by Fatty Acid Synthase Induces Apoptosis in Tumor Cells by Remodeling Cell Membranes, Inhibiting Signaling Pathways, and Reprogramming Gene Expression.
Authors: Ventura, Richard and Mordec, Kasia and Waszczuk, Joanna and Wang, Zhaoti and Lai, Julie and Fridlib, Marina and Buckley, Douglas and Kemble, George and Heuer, Timothy S
Journal: EBioMedicine (2015): 808-24
Authors: Ventura, Richard and Mordec, Kasia and Waszczuk, Joanna and Wang, Zhaoti and Lai, Julie and Fridlib, Marina and Buckley, Douglas and Kemble, George and Heuer, Timothy S
Journal: EBioMedicine (2015): 808-24
Sucrose esters increase drug penetration, but do not inhibit p-glycoprotein in caco-2 intestinal epithelial cells.
Authors: Kiss, Lóránd and Hellinger, Éva and Pilbat, Ana-Maria and Kittel, Ágnes and Török, Zsolt and Füredi, András and Szakács, Gergely and Veszelka, Szilvia and Sipos, Péter and Ózsvári, Béla and Puskás, László G and Vastag, Monika and Szabó-Révész, Piroska and Deli, Mária A
Journal: Journal of pharmaceutical sciences (2014): 3107-19
Authors: Kiss, Lóránd and Hellinger, Éva and Pilbat, Ana-Maria and Kittel, Ágnes and Török, Zsolt and Füredi, András and Szakács, Gergely and Veszelka, Szilvia and Sipos, Péter and Ózsvári, Béla and Puskás, László G and Vastag, Monika and Szabó-Révész, Piroska and Deli, Mária A
Journal: Journal of pharmaceutical sciences (2014): 3107-19
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