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FITC-streptavidin conjugate
Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules since streptavidin has a very high binding affinity for biotin. This FITC-streptavidin conjugate comprises streptavidin (as the biotin-binding protein) with FITC covalently attached (as the fluorescent label). Our FITC-streptavidin conjugate was prepared using AAT Bioquest's proprietary labeling technology. It demonstrated much brighter signal compared to the similar FITC-streptavidin conjugates from other commercial sources, thus can significantly increase assay sensitivities. It is commonly used as a second step reagent for indirect immunofluorescent staining, when used in conjunction with biotinylated primary antibodies. It is a valuable tool for biotin-streptavidin-based biological assays and tests using flow cytometry with a FITC filter set. A variety of the complementary biotinylated reagents are available from numerous commercial vendors.
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's FITC-streptavidin conjugate (Green). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
HeLa cells were incubated with mouse anti-tubulin and biotin goat anti-mouse IgG followed by AAT's FITC-streptavidin conjugate (Green). Cell nuclei were stained with Hoechst 33342 (Blue, Cat#17530).
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
169101 mg
Price
 
Physical properties
SolventWater
Spectral properties
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
73000
Excitation (nm)491
Emission (nm)516
Quantum yield
0.92
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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Page updated on October 8, 2025