Fluo-2, AM

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-2 is the parent compound of Fluo-3 and Fluo-4. These fluorescent calcium indicators have calcium-dependent fluorescence.

Protocol

COA

Catalog	Size	Price	Quantity
20494	10x50 ug	Price
20495	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluo-2, AM to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	94.254 µL	471.271 µL	942.543 µL	4.713 mL	9.425 mL
5 mM	18.851 µL	94.254 µL	188.509 µL	942.543 µL	1.885 mL
10 mM	9.425 µL	47.127 µL	94.254 µL	471.271 µL	942.543 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1060.96 g/mol	=	mL	x	mM	=	mmol

Citations

View all 12 citations: Citation Explorer

GC-globulin/vitamin D-binding protein is required for pancreatic $\alpha$ cell adaptation to metabolic stress

Authors:

Viloria, K and Nasteska, D and Ast, J and Hasib, A and Cuozzo, F and Heising, S and Briant, L and Hewison, M and Hodson, D

Journal:

Diabetes (2022)

Mitochonic acid 5 regulates mitofusin 2 to protect microglia

Authors:

Tan, Jian and Chen, Shuang-Xi and Lei, Qing-Yun and Yi, Shan-Qing and Wu, Na and Wang, Yi-Lin and Xiao, Zi-Jian and Wu, Heng and others,

Journal:

Neural Regeneration Research (2021): 1813

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling

Authors:

Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling

Journal:

The International Journal of Biochemistry & Cell Biology (2017)

Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells

Authors:

Lu, Jiang and Yao, Xue-qin and Luo, Xin and Wang, Yu and Chung, Sookja Kim and Tang, He-xin and Cheung, Chi Wai and Wang, Xian-yu and Meng, Chen and Li, Qing and others, undefined

Journal:

Neural Regeneration Research (2017): 945

Obtaining spontaneously beating cardiomyocyte-like cells from adipose-derived stromal vascular fractions cultured on enzyme-crosslinked gelatin hydrogels

Authors:

Yang, Gang and Xiao, Zhenghua and Ren, Xiaomei and Long, Haiyan and Ma, Kunlong and Qian, Hong and Guo, Yingqiang

Journal:

Scientific Reports (2017): 41781

References

View all 53 references: Citation Explorer

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics

Authors:

Loughrey CM, MacEachern KE, Cooper J, Smith GL.

Journal:

Cell Calcium (2003): 1

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry

Authors:

Patel H, Porter RH, Palmer AM, Croucher MJ.

Journal:

Br J Pharmacol (2003): 671

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification

Authors:

Zhang Z, Kitching P.

Journal:

J Virol Methods (2000): 187

Physical properties

Dissociation constant (K_d, nM)	232
Molecular weight	1060.96
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	108964-32-5

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC
Emission	FITC
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490
Emission	525
Cutoff	515
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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Page updated on October 12, 2025