Name: Fluo-3, AM *Bulk package* *CAS 121714-22-5*
Brand: AAT Bioquest
SKU: 21012
Price: 4627 USD
Availability: InStock

Fluo-3, AM

Bulk package; CAS 121714-22-5

Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Rhod-2 are most commonly used among the visible light-excitable calcium indicators. Fluo-3 indicators are widely used in flow cytometry and confocal laser-scanning microscopy. More recently, Fluo-3, AM has been extensively used in cell-based high-throughput screening assays for functional GPCR assays. Fluo-3 is essentially nonfluorescent unless bound to Ca2+ and exhibits a quantum yield at saturating Ca2+ of ~0.14 and a Kd for Ca2+ of 390 nM.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21012	50 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluo-3, AM *Bulk package* *CAS 121714-22-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	88.507 µL	442.537 µL	885.073 µL	4.425 mL	8.851 mL
5 mM	17.701 µL	88.507 µL	177.015 µL	885.073 µL	1.77 mL
10 mM	8.851 µL	44.254 µL	88.507 µL	442.537 µL	885.073 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1129.85 g/mol	=	mL	x	mM	=	mmol

Citations

View all 16 citations: Citation Explorer

Moso Bamboo’s Survival Strategy Against Chilling Stress in Signaling Dynamics

Authors:

Jing, Xiong and Cai, Chunju and Geng, Pengfei and Wang, Yi

Journal:

Forests (2024): 2132

Comprehensive review of indicators and techniques for optical mapping of intracellular calcium ions

Authors:

Sheng, Chu-Qiao and Wu, Shuang-Shuang and Cheng, Yong-Kang and Wu, Yao and Li, Yu-Mei

Journal:

Cerebral Cortex (2024): bhae346

Microfluidic organ chip of fluid--solid dynamic curved interface

Authors:

Su, Haoran and Ma, Tianxiang and Liu, Xiao and Wang, Li and Shu, Fangjun and Liang, Zhuqing and Zhang, Dongrui and Zhang, Xing and Li, Kexin and Wang, Min and others,

Journal:

Applied Physics Reviews (2024)

Downregulation of connexin 43-based gap junctions underlies propofol-induced excessive relaxation in hypertensive vascular smooth muscle cells

Authors:

Zeng, Weiqi and Deng, Zhizhao and Gao, Yingxin and Sun, Guoliang and Li, Xianlong and Yuan, Dongdong

Journal:

Cell Communication and Signaling (2023): 1--14

Calreticulin regulates TGF-$\beta$1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling

Authors:

Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling

Journal:

The International Journal of Biochemistry \& Cell Biology (2017): 103--113

References

View all 53 references: Citation Explorer

A flow cytometric comparison of Indo-1 to fluo-3 and Fura Red excited with low power lasers for detecting Ca(2+) flux

Authors:

Bailey S, Macardle PJ.

Journal:

J Immunol Methods (2006): 220

Functional fluo-3/AM assay on P-glycoprotein transport activity in L1210/VCR cells by confocal microscopy

Authors:

Orlicky J, Sulova Z, Dovinova I, Fiala R, Zahradnikova A, Jr., Breier A.

Journal:

Gen Physiol Biophys (2004): 357

Comparison of human recombinant adenosine A2B receptor function assessed by Fluo-3-AM fluorometry and microphysiometry

Authors:

Patel H, Porter RH, Palmer AM, Croucher MJ.

Journal:

Br J Pharmacol (2003): 671

Measurement of the dissociation constant of Fluo-3 for Ca2+ in isolated rabbit cardiomyocytes using Ca2+ wave characteristics

Authors:

Loughrey CM, MacEachern KE, Cooper J, Smith GL.

Journal:

Cell Calcium (2003): 1

A sensitive method for the detection of foot and mouth disease virus by in situ hybridisation using biotin-labelled oligodeoxynucleotides and tyramide signal amplification

Authors:

Zhang Z, Kitching P.

Journal:

J Virol Methods (2000): 187

Physical properties

Dissociation constant (K_d, nM)	390
Molecular weight	1129.85
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	86,000 ¹
Excitation (nm)	506
Emission (nm)	515
Quantum yield	0.15 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	121714-22-5

Instrument settings

Fluorescence microscope
Excitation	FITC
Emission	FITC
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490
Emission	525
Cutoff	515
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

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Page updated on October 6, 2025