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Fluo-3FF, AM
UltraPure grade; Cell permeant
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Rhod-2 are most commonly used among the visible light-excitable calcium indicators. This cell-permeant Fluo-3FF is an analog of Fluo-3 AM with much lower calcium binding affinity, Kd ~10 µM. Like Fluo-3 AM, this indicator is essentially nonfluorescent, but exhibits strong fluorescence enhancement with no spectral shift upon entering cells and binding calcium.
Chemical structure for Fluo-3FF, AM *UltraPure grade* *Cell permeant*
Chemical structure for Fluo-3FF, AM *UltraPure grade* *Cell permeant*
CatalogSize
Price
Quantity
2101410x50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)10000
Molecular weight1151.80
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
86,000
1
Excitation (nm)506
Emission (nm)515
Quantum yield
0.15
1
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Fluorescence microscope
ExcitationFITC
EmissionFITC
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation490
Emission525
Cutoff515
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on July 12, 2023