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Fluo-4, Pentapotassium Salt

Chemical structure for Fluo-4, Pentapotassium Salt
Chemical structure for Fluo-4, Pentapotassium Salt
Ordering information
Price ()
Catalog Number20555
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
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Physical properties
Dissociation constant (Kd, nM)345
Molecular weight927.08
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)82000
Excitation (nm)495
Emission (nm)528
Quantum yield0.161
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C)
UNSPSC12352200
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Show More (83)

OverviewpdfSDSpdfProtocol


Molecular weight
927.08
Dissociation constant (Kd, nM)
345
Extinction coefficient (cm -1 M -1)
82000
Excitation (nm)
495
Emission (nm)
528
Quantum yield
0.161
Calcium measurement is critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding calcium have enabled researchers to investigate changes in intracellular free calcium concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Fluo-3 and Fluo-4 are most commonly used among the visible light-excitable calcium indicators. Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels. Cells may be physically loaded with the cell-impermeant salt forms of these indicators using patch pipette or microinjection. These indicators are useful for fluorescence and confocal microscopy, flow cytometry, and microplate screening applications.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluo-4, Pentapotassium Salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM107.866 µL539.328 µL1.079 mL5.393 mL10.787 mL
5 mM21.573 µL107.866 µL215.731 µL1.079 mL2.157 mL
10 mM10.787 µL53.933 µL107.866 µL539.328 µL1.079 mL

Molarity calculator

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Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)82000
Excitation (nm)495
Emission (nm)528
Quantum yield0.161

Citations


View all 1 citations: Citation Explorer
Succinate, increased in metabolic syndrome, activates GPR91 receptor signaling in urothelial cells
Authors: Mossa, Abubakr H and Flores, Monica Velasquez and Cammisotto, Philippe G and Campeau, Lysanne
Journal: Cellular Signalling (2017)