Name: Fluo-5F, AM *Cell permeant*
Brand: AAT Bioquest
SKU: 20560
Price: 232 USD
Availability: InStock

Fluo-5F, AM

Cell permeant

Fluo-5F is an analog of Fluo-4 with lower calcium-binding affinity (Kd = ~2.3 uM), making it suitable for detecting intracellular calcium levels in the range of 1 µM to 1 mM that would saturate the response of Fluo-4. Cells may be loaded with Fluo-5F AM ester by adding the dissolved indicator directly to dishes containing cultured cells. It is compatible with excitation at 488 nm by argon-ion laser sources, making Fluo-5F useful for confocal microscopy, flow cytometry, and microplate screening applications. It has excitation and emission wavelengths at 494 and 516 nm respectively. Upon calcium binding, its fluorescence intensity increases by >100 fold.

Protocol

SDS

Catalog	Size	Price	Quantity
20560	10x50 ug	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluo-5F, AM *Cell permeant* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	90.834 µL	454.17 µL	908.339 µL	4.542 mL	9.083 mL
5 mM	18.167 µL	90.834 µL	181.668 µL	908.339 µL	1.817 mL
10 mM	9.083 µL	45.417 µL	90.834 µL	454.17 µL	908.339 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1100.91 g/mol	=	mL	x	mM	=	mmol

Citations

View all 14 citations: Citation Explorer

Mathematical model of the zebrafish ventricular cardiomyocyte action potential and calcium transient

Authors:

Cestariolo, Ludovica and Long, Zachary D and Verkerk, Arie O and Ferrero, Jose M and Quinn, T Alexander and Matas, Jose F Rodriguez

Journal:

The Journal of Physiology (2025)

Biophysical Studies of Endocrine and Synaptic Release

Authors:

Cheng, Jinbo

Somatostatin modulation of initial fusion pores in Ca2+-triggered exocytosis from mouse chromaffin cells

Authors:

Cheng, Jinbo and Jackson, Meyer B

Journal:

The Journal of Physiology (2024)

Vitamin D is an endogenous partial agonist of the transient receptor potential vanilloid 1 channel

Authors:

Long, Wentong and Fatehi, Mohammad and Soni, Shubham and Panigrahi, Rashmi and Philippaert, Koenraad and Yu, Yi and Kelly, Rees and Boonen, Brett and Barr, Amy and Golec, Dominic and others,

Journal:

The Journal of Physiology (2020): 4321--4338

Calreticulin regulates TGF-β1-induced epithelial mesenchymal transition through modulating Smad signaling and calcium signaling

Authors:

Wu, Yanjiao and Xu, Xiaoli and Ma, Lunkun and Yi, Qian and Sun, Weichao and Tang, Liling

Journal:

The International Journal of Biochemistry & Cell Biology (2017)

References

View all 71 references: Citation Explorer

Direct detection of SERCA calcium transport and small-molecule inhibition in giant unilamellar vesicles

Authors:

Bian T, Autry JM, Casemore D, Li J, Thomas DD, He G, Xing C.

Journal:

Biochem Biophys Res Commun (2016): 206

Single cell and subcellular measurements of intracellular Ca(2)(+) concentration

Authors:

McCarron JG, Olson ML, Chalmers S, Girkin JM.

Journal:

Methods Mol Biol (2013): 239

Astrocyte calcium signals at Schaffer collateral to CA1 pyramidal cell synapses correlate with the number of activated synapses but not with synaptic strength

Authors:

Honsek SD, Walz C, Kafitz KW, Rose CR.

Journal:

Hippocampus (2012): 29

Preferential loading of bergmann glia with synthetic acetoxymethyl calcium dyes

Authors:

Hoogl, undefined and TM, Kuhn B, Wang SS.

Journal:

Cold Spring Harb Protoc (2011): 1228

Visualization and quantification of endoplasmic reticulum Ca2+ in renal cells using confocal microscopy and Fluo5F

Authors:

Eaddy AC, Schnellmann RG.

Journal:

Biochem Biophys Res Commun (2011): 424

Physical properties

Dissociation constant (K_d, nM)	2300
Molecular weight	1100.91
Solvent	DMSO

Spectral properties

Excitation (nm)	494
Emission (nm)	516

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Instrument settings

Flow cytometer
Excitation	488 nm laser
Emission	530/30 nm filter
Instrument specification(s)	FITC channel

Fluorescence microscope
Excitation	FITC
Emission	FITC
Recommended plate	Black wall/clear bottom

Fluorescence microplate reader
Excitation	490
Emission	525
Cutoff	515
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Bottom read mode/Programmable liquid handling

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Calcium Detection Probes & Assay Kits

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Page updated on October 30, 2025