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Fluo-5F, AM
Cell permeant
Fluo-5F is an analog of Fluo-4 with lower calcium-binding affinity (Kd = ~2.3 uM), making it suitable for detecting intracellular calcium levels in the range of 1 µM to 1 mM that would saturate the response of Fluo-4. Cells may be loaded with Fluo-5F AM ester by adding the dissolved indicator directly to dishes containing cultured cells. It is compatible with excitation at 488 nm by argon-ion laser sources, making Fluo-5F useful for confocal microscopy, flow cytometry, and microplate screening applications. It has excitation and emission wavelengths at 494 and 516 nm respectively. Upon calcium binding, its fluorescence intensity increases by >100 fold.
Chemical structure for Fluo-5F, AM *Cell permeant*
Chemical structure for Fluo-5F, AM *Cell permeant*
CatalogSize
Price
Quantity
2056010x50 ug
Price
 
Physical properties

Dissociation constant (Kd, nM)2300
Molecular weight1100.91
SolventDMSO
Spectral properties

Excitation (nm)494
Emission (nm)516
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings

Flow cytometer
Excitation488 nm laser
Emission530/30 nm filter
Instrument specification(s)FITC channel

Fluorescence microscope
ExcitationFITC
EmissionFITC
Recommended plateBlack wall/clear bottom

Fluorescence microplate reader
Excitation490
Emission525
Cutoff515
Recommended plateBlack wall/clear bottom
Instrument specification(s)Bottom read mode/Programmable liquid handling
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Page updated on October 5, 2025