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Fluo-5F, pentapotassium Salt
Cell impermeant
Fluo-5F is an analog of Fluo-4 with lower calcium-binding affinity (Kd = ~2.3 uM), making it suitable for detecting intracellular calcium levels in the range of 1 µM to 1 mM that would saturate the response of Fluo-4. Cells may be physically loaded with the cell-impermeant Fluo-5F using patch pipette or microinjection. More commonly Fluo-5F AM ester (#20560) may be directly loaded into live cells by adding the dissolved indicator directly to dishes containing the cultured cells. It is compatible with excitation at 488 nm by argon-ion laser sources, making Fluo-5F useful for confocal microscopy, flow cytometry, and microplate screening applications. It has excitation and emission wavelengths at 494 and 516 nm respectively. Upon calcium binding, its fluorescence intensity increases by >100 fold.
| Catalog | Size | Price | Quantity |
|---|---|---|---|
| 20562 | 500 ug | Price |
Physical properties
| Dissociation constant (Kd, nM) | 2300 |
| Molecular weight | 931.05 |
| Solvent | Water |
Spectral properties
| Excitation (nm) | 494 |
| Emission (nm) | 516 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
Contact us
| Telephone | |
| Fax | |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Technical Support | Contact us |
| Request quotation | Request |
| Purchase order | Send to sales@aatbio.com |
| Shipping | Standard overnight for United States, inquire for international |
Page updated on October 25, 2025