Calculator
Fluo-8®, potassium salt
Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ∼490/∼520 nm. Fluo-8® AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading. In addition, Fluo-8® AM is two times brighter than Fluo-4 AM and four times brighter than Fluo-3 AM. AAT Bioquest offers a set of our outstanding Fluo-8® reagents with different calcium-binding affinities (Fluo-8® Kd = 389 nM; Fluo-8H™ Kd = 232 nM; Fluo-8L™ Kd = 1.86 µM; Fluo-8FF™ Kd = 10 µM). We also offer versatile packing sizes to meet your special needs (e.g., 1 mg, 10x50 µg, 20x50 µg, and HTS packages) with no additional packaging charge.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of Fluo-8®, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 114.016 µL | 570.08 µL | 1.14 mL | 5.701 mL | 11.402 mL |
| 5 mM | 22.803 µL | 114.016 µL | 228.032 µL | 1.14 mL | 2.28 mL |
| 10 mM | 11.402 µL | 57.008 µL | 114.016 µL | 570.08 µL | 1.14 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| Fluo-8®, sodium salt | 495 | 516 | 23430 | 0.161 | 1.076 | 0.769 |
| Fluo-8FF™, potassium salt | 495 | 516 | 23430 | 0.161 | 1.076 | 0.769 |
| Fluo-8L™, potassium salt | 495 | 516 | 23430 | 0.161 | 1.076 | 0.769 |
Citations
View all 155 citations: Citation Explorer
The Role of Orai1 in Regulating Sarcoplasmic Calcium Release, Mitochondrial Morphology and Function in Myostatin Deficient Skeletal Muscle
Authors: Sztretye, M{\'o}nika and Singl{\'a}r, Zolt{\'a}n and Balogh, Norbert and Kis, Gr{\'e}ta and Szentesi, P{\'e}ter and Angyal, {\'A}gnes and Balatoni, Ildik{\'o} and Csernoch, L{\'a}szl{\'o} and Dienes, Beatrix
Journal: Frontiers in physiology (2020): 601090
Authors: Sztretye, M{\'o}nika and Singl{\'a}r, Zolt{\'a}n and Balogh, Norbert and Kis, Gr{\'e}ta and Szentesi, P{\'e}ter and Angyal, {\'A}gnes and Balatoni, Ildik{\'o} and Csernoch, L{\'a}szl{\'o} and Dienes, Beatrix
Journal: Frontiers in physiology (2020): 601090
The sixth transmembrane segment is a major gating component of the TMEM16A calcium-activated chloride channel
Authors: Peters, Christian J and Gilchrist, John M and Tien, Jason and Bethel, Neville P and Qi, Lijun and Chen, Tingxu and Wang, Lynn and Jan, Yuh Nung and Grabe, Michael and Jan, Lily Y
Journal: Neuron (2018): 1063--1077
Authors: Peters, Christian J and Gilchrist, John M and Tien, Jason and Bethel, Neville P and Qi, Lijun and Chen, Tingxu and Wang, Lynn and Jan, Yuh Nung and Grabe, Michael and Jan, Lily Y
Journal: Neuron (2018): 1063--1077
Aryl-and alkyl-phosphorus-containing flame retardants induced mitochondrial impairment and cell death in Chinese hamster ovary (CHO-k1) cells
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786
Authors: Huang, Chao and Li, Na and Yuan, Shengwu and Ji, Xiaoya and Ma, Mei and Rao, Kaifeng and Wang, Zijian
Journal: Environmental Pollution (2017): 775--786
Ex vivo replication of phenotypic functions of osteocytes through biomimetic 3D bone tissue construction
Authors: Sun, Qiaoling and Choudhary, Saba and Mannion, Ciaran and Kissin, Yair and Zilberberg, Jenny and Lee, Woo Y
Journal: Bone (2017)
Authors: Sun, Qiaoling and Choudhary, Saba and Mannion, Ciaran and Kissin, Yair and Zilberberg, Jenny and Lee, Woo Y
Journal: Bone (2017)
Non-steroidal anti-inflammatory drug delays corneal wound healing by reducing production of 12-hydroxyheptadecatrienoic acid, a ligand for leukotriene B4 receptor 2
Authors: Iwamoto, Satoshi and Koga, Tomoaki and Ohba, Mai and Okuno, Toshiaki and Koike, Masato and Murakami, Akira and Matsuda, Akira and Yokomizo, Takehiko
Journal: Scientific Reports (2017)
Authors: Iwamoto, Satoshi and Koga, Tomoaki and Ohba, Mai and Okuno, Toshiaki and Koike, Masato and Murakami, Akira and Matsuda, Akira and Yokomizo, Takehiko
Journal: Scientific Reports (2017)
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