Fluo-8FF™, potassium salt

Calcium measurements are critical for numerous biological investigations. Fluorescent probes that show spectral responses upon binding Ca2+ have enabled researchers to investigate changes in intracellular free Ca2+ concentrations by using fluorescence microscopy, flow cytometry, fluorescence spectroscopy, and fluorescence microplate readers. Fluo-3 AM and Fluo-4 AM are most commonly used among the visible light-excitable calcium indicators for live-cell calcium imaging. However, Fluo-3 AM and Fluo-4 AM are only moderately fluorescent in live cells upon esterase hydrolysis and require harsh cell loading conditions to maximize their cellular calcium responses. Fluo-8® dyes are developed to improve cell loading and calcium response while maintaining the convenient Fluo-3 and Fluo-4 spectral wavelengths of Ex/Em = ≤490/≤520 nm. Fluo-8® AM can be loaded into cells at room temperature, while Fluo-3 AM and Fluo-4 AM require 37°C for cell loading. In addition, Fluo-8® AM is two times brighter than Fluo-4 AM and four times brighter than Fluo-3 AM. AAT Bioquest offers a set of our outstanding Fluo-8® reagents with different calcium-binding affinities (Fluo-8® Kd = 389 nM; Fluo-8H™ Kd = 232 nM; Fluo-8L™ Kd = 1.86 µM; Fluo-8FF™ Kd = 10 µM). We also offer versatile packing sizes to meet your special needs (e.g., 1 mg, 10x50 µg, 20x50 µg, and HTS packages) with no additional packaging charge.

Protocol

COA

Catalog	Size	Price	Quantity
21102	10x50 ug	Price
21103	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluo-8FF™, potassium salt to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	109.523 µL	547.615 µL	1.095 mL	5.476 mL	10.952 mL
5 mM	21.905 µL	109.523 µL	219.046 µL	1.095 mL	2.19 mL
10 mM	10.952 µL	54.762 µL	109.523 µL	547.615 µL	1.095 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	913.05 g/mol	=	mL	x	mM	=	mmol

Citations

View all 154 citations: Citation Explorer

The sixth transmembrane segment is a major gating component of the TMEM16A calcium-activated chloride channel

Authors:

Peters, Christian J and Gilchrist, John M and Tien, Jason and Bethel, Neville P and Qi, Lijun and Chen, Tingxu and Wang, Lynn and Jan, Yuh Nung and Grabe, Michael and Jan, Lily Y

Journal:

Neuron (2018): 1063--1077

High-throughput screen detects calcium signaling dysfunction in typical sporadic autism spectrum disorder

Authors:

Schmunk, Galina and Nguyen, Rachel L and Ferguson, David L and Kumar, Kenny and Parker, Ian and Gargus, J Jay

Journal:

Scientific Reports (2017): 40740

Ex vivo replication of phenotypic functions of osteocytes through biomimetic 3D bone tissue construction

Authors:

Sun, Qiaoling and Choudhary, Saba and Mannion, Ciaran and Kissin, Yair and Zilberberg, Jenny and Lee, Woo Y

Journal:

Bone (2017)

Non-steroidal anti-inflammatory drug delays corneal wound healing by reducing production of 12-hydroxyheptadecatrienoic acid, a ligand for leukotriene B4 receptor 2

Authors:

Iwamoto, Satoshi and Koga, Tomoaki and Ohba, Mai and Okuno, Toshiaki and Koike, Masato and Murakami, Akira and Matsuda, Akira and Yokomizo, Takehiko

Journal:

Scientific Reports (2017)

Bystander effects elicited by single-cell photo-oxidative blue-light stimulation in retinal pigment epithelium cell networks

Authors:

Ishii, Masaaki and Rohrer, Bärbel

Journal:

Cell Death Discovery (2017): 16071

Physical properties

Dissociation constant (K_d, nM)	10000
Molecular weight	913.05
Solvent	Water

Spectral properties

Correction factor (260 nm)	1.076
Correction factor (280 nm)	0.769
Extinction coefficient (cm ^-1 M ^-1)	23430
Excitation (nm)	495
Emission (nm)	516
Quantum yield	0.16 ¹

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Shipping	Standard overnight for United States, inquire for international

Page updated on October 12, 2025