Name: Fluorescamine *CAS 38183-12-9*
Brand: AAT Bioquest
SKU: 820
Price: 115 USD
Availability: InStock

Fluorescamine

CAS 38183-12-9

Fluorescamine is a popular amine-fluorogenic reagent for determining protein concentrations in solutions and on gels. It is also used to analyze low molecular weight amines by TLC, HPLC and capillary electrophoresis. Fluorescamine is nonfluorescent but readily reacts with primary aliphatic amines, including those in peptides and proteins, to yield a strongly blue-green fluorescent adduct that can be excited by UV light. The amine adduct has absorption maximum at 385 nm and fluorescence maximum at 486 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
820	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescamine *CAS 38183-12-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	359.376 µL	1.797 mL	3.594 mL	17.969 mL	35.938 mL
5 mM	71.875 µL	359.376 µL	718.752 µL	3.594 mL	7.188 mL
10 mM	35.938 µL	179.688 µL	359.376 µL	1.797 mL	3.594 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	278.26 g/mol	=	mL	x	mM	=	mmol

References

View all 41 references: Citation Explorer

Fluorescence of aromatic amines and their fluorescamine derivatives for detection of explosive vapors

Authors:

Eastwood D, Fern and ez C, Yoon BY, Sheaff CN, Wai CM.

Journal:

Appl Spectrosc (2006): 958

Fluorimetric determination of histamine in fish using micellar media and fluorescamine as labelling reagent

Authors:

Adamou R, Coly A, Douabale SE, Saleck ML, Gaye-Seye MD, Tine A.

Journal:

J Fluoresc (2005): 679

Determination of 4-amino-m-cresol and 5-amino-o-cresol by high performance liquid chromatography and fluorescence derivatization using fluorescamine

Authors:

Eggenreich K, Zach E, Beck H, Wintersteiger R.

Journal:

J Biochem Biophys Methods (2004): 35

Chiral separation of fluorescamine-labeled amino acids using microfabricated capillary electrophoresis devices for extraterrestrial exploration

Authors:

Skelley AM, Mathies RA.

Journal:

J Chromatogr A (2003): 191

LC determination of aminoglutethimide enantiomers as dansyl and fluorescamine derivatives in tablet formulations

Authors:

Cesur N, Apak TI, Aboul-Enein HY, Ozkirimli S.

Journal:

J Pharm Biomed Anal (2002): 487

Physical properties

Molecular weight	278.26
Solvent	DMSO

Spectral properties

Absorbance (nm)	237
Excitation (nm)	382
Emission (nm)	480

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	38183-12-9

Instrument settings

Fluorescence microplate reader
Excitation	382 nm
Emission	480 nm
Cutoff	410 nm
Recommended plate	Solid black

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Page updated on October 10, 2025