Fluorescein-12-dATP 1 mM in Tris Buffer (pH 7.5)

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Physical properties

Molecular weight	1014.72
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

1014.72

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

80000¹

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.7900¹, 0.95²

Fluorescein-12-dATP might be incorporated into a DNA sequence as its natural counterpart dATP to incorporate a fluorescein tag in a DNA molecule. It can be used for the direct enzymatic labeling of DNA/cDNA, e.g., by nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. The resulting fluorescein-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. The linker between fluorescein tag and adenine has been optimized for the optimal substrate incorporation and labeling efficiency. The labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein-12-dATP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	98.549 µL	492.747 µL	985.494 µL	4.927 mL	9.855 mL
5 mM	19.71 µL	98.549 µL	197.099 µL	985.494 µL	1.971 mL
10 mM	9.855 µL	49.275 µL	98.549 µL	492.747 µL	985.494 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein-12-dUTP 1 mM in Tris Buffer (pH 7.5) CAS 214154-36-6	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dUTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dCTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dGTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

Images

Figure 1. Chemical structure for Fluorescein-12-dATP *1 mM in Tris Buffer (pH 7.5)* .

References

View all 1 references: Citation Explorer

Highly sensitive fluorometric determination of thrombin by on-chip signal amplification initiated by terminal deoxynucleotidyl transferase.
Authors: Wen, Dongxiao and He, Minhui and Ma, Kefeng and Cui, Ying and Kong, Jinming and Yang, Huaixia and Liu, Qingyun
Journal: Mikrochimica acta (2018): 380