Fluorescein-12-dCTP 1 mM in Tris Buffer (pH 7.5)

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Physical properties

Molecular weight	991.69
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

991.69

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

80000¹

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.7900¹, 0.95²

Fluorescein-12-dCTP might be incorporated into a DNA sequence as its natural counterpart dCTP to incorporate a fluorescein tag in a DNA molecule. It can be used for the direct enzymatic labeling of DNA/cDNA, e.g., by PCR or Nick Translation. The resulting fluorescein-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. The linker between fluorescein tag and cytidine has been optimized for the optimal substrate incorporation and labeling efficiency.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein-12-dCTP *1 mM in Tris Buffer (pH 7.5)* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	100.838 µL	504.19 µL	1.008 mL	5.042 mL	10.084 mL
5 mM	20.168 µL	100.838 µL	201.676 µL	1.008 mL	2.017 mL
10 mM	10.084 µL	50.419 µL	100.838 µL	504.19 µL	1.008 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)
Fluorescein-12-dUTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dATP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35
Fluorescein-12-dGTP 1 mM in Tris Buffer (pH 7.5)	498	517	80000¹	0.7900¹, 0.95²	0.32	0.35

Images

Figure 1. Chemical structure for Fluorescein-12-dCTP *1 mM in Tris Buffer (pH 7.5)* .

References

View all 18 references: Citation Explorer

Development and Preliminary Application of Multiplex Loop-Mediated Isothermal Amplification Coupled With Lateral Flow Biosensor for Detection of Mycobacterium tuberculosis Complex.
Authors: Wang, Xingyun and Wang, Guirong and Wang, Yacui and Quan, Shuting and Qi, Hui and Sun, Lin and Shen, Chen and Huang, Hairong and Jiao, Weiwei and Shen, Adong
Journal: Frontiers in cellular and infection microbiology (2021): 666492

Human Pol ζ purified with accessory subunits is active in translesion DNA synthesis and complements Pol η in cisplatin bypass.
Authors: Lee, Young-Sam and Gregory, Mark T and Yang, Wei
Journal: Proceedings of the National Academy of Sciences of the United States of America (2014): 2954-9

[Detection of heterogeneity and evolution of subclones in t(8;21) AML by QM-FISH].
Authors: Wang, Ying-chan and Hu, Lin-ping and Lin, Dong and Li, Cheng-wen and Yuan, Tian and Jia, Yu-jiao and Tian, Zheng and Tang, Ke-jing and Wang, Min and Wang, Jian-xiang
Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (2013): 844-50

A comprehensive HBV array for the detection of HBV mutants and genotype.
Authors: Chan, Kaimin and Yam, Irene and Yuen, John and Yuen, Man-Fung and Lai, Ching-Lung and Alexander, Graeme J and Chan, Tai-Kwong and Chan, Vivian
Journal: Clinical biochemistry (2011): 1253-60

Cationic conjugated polymers for optical detection of DNA methylation, lesions, and single nucleotide polymorphisms.
Authors: Duan, Xinrui and Liu, Libing and Feng, Fude and Wang, Shu
Journal: Accounts of chemical research (2010): 260-70

Development of a universal chemiluminometric genotyping method for high-throughput detection of 7 LDLR gene mutations in Greek population.
Authors: Glynou, Kyriaki and Laios, Eleftheria and Drogari, Euridiki and Tsaoussis, Vassilis
Journal: Clinical biochemistry (2008): 335-42

High-throughput microtiter well-based chemiluminometric genotyping of 15 HBB gene mutations in a dry-reagent format.
Authors: Glynou, Kyriaki and Kastanis, Petros and Boukouvala, Sotiria and Tsaoussis, Vassilis and Ioannou, Penelope C and Christopoulos, Theodore K and Traeger-Synodinos, Joanne and Kanavakis, Emmanuel
Journal: Clinical chemistry (2007): 384-91

Methylation-dependent fragment separation: direct detection of DNA methylation by capillary electrophoresis of PCR products from bisulfite-converted genomic DNA.
Authors: Boyd, Victoria L and Moody, Kristina I and Karger, Achim E and Livak, Kenneth J and Zon, Gerald and Burns, John W
Journal: Analytical biochemistry (2006): 266-73

Quantitation of fluorescent nucleotide incorporation by capillary gel electrophoresis and laser-induced fluorescence detection.
Authors: Cruickshank, K A
Journal: Analytical biochemistry (1999): 21-31

DNA copy number changes in childhood acute lymphoblastic leukemia.
Authors: Larramendy, M L and Huhta, T and Heinonen, K and Vettenranta, K and Mahlamäki, E and Riikonen, P and Saarinen-Pihkala, U M and Knuutila, S
Journal: Haematologica (1998): 890-5