Fluorescein-12-dCTP *1 mM in Tris Buffer (pH 7.5)*

Fluorescein-12-dCTP

1 mM in Tris Buffer (pH 7.5)

Fluorescein-12-dCTP might be incorporated into a DNA sequence as its natural counterpart dCTP to incorporate a fluorescein tag in a DNA molecule. It can be used for the direct enzymatic labeling of DNA/cDNA, e.g., by PCR or Nick Translation. The resulting fluorescein-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. The linker between fluorescein tag and cytidine has been optimized for the optimal substrate incorporation and labeling efficiency.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17035	25 nmoles	Price

References

View all 18 references: Citation Explorer

Development and Preliminary Application of Multiplex Loop-Mediated Isothermal Amplification Coupled With Lateral Flow Biosensor for Detection of Mycobacterium tuberculosis Complex.

Authors:

Wang, Xingyun and Wang, Guirong and Wang, Yacui and Quan, Shuting and Qi, Hui and Sun, Lin and Shen, Chen and Huang, Hairong and Jiao, Weiwei and Shen, Adong

Journal:

Frontiers in cellular and infection microbiology (2021): 666492

Human Pol ζ purified with accessory subunits is active in translesion DNA synthesis and complements Pol η in cisplatin bypass.

Authors:

Lee, Young-Sam and Gregory, Mark T and Yang, Wei

Journal:

Proceedings of the National Academy of Sciences of the United States of America (2014): 2954-9

[Detection of heterogeneity and evolution of subclones in t(8;21) AML by QM-FISH].

Authors:

Wang, Ying-chan and Hu, Lin-ping and Lin, Dong and Li, Cheng-wen and Yuan, Tian and Jia, Yu-jiao and Tian, Zheng and Tang, Ke-jing and Wang, Min and Wang, Jian-xiang

Journal:

Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (2013): 844-50

A comprehensive HBV array for the detection of HBV mutants and genotype.

Authors:

Chan, Kaimin and Yam, Irene and Yuen, John and Yuen, Man-Fung and Lai, Ching-Lung and Alexander, Graeme J and Chan, Tai-Kwong and Chan, Vivian

Journal:

Clinical biochemistry (2011): 1253-60

Cationic conjugated polymers for optical detection of DNA methylation, lesions, and single nucleotide polymorphisms.

Authors:

Duan, Xinrui and Liu, Libing and Feng, Fude and Wang, Shu

Journal:

Accounts of chemical research (2010): 260-70

Physical properties

Molecular weight	991.69
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 5, 2025