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Fluorescein-12-dGTP
1 mM in Tris Buffer (pH 7.5)
Fluorescein-12-dGTP might be incorporated into a DNA sequence as its natural counterpart dGTP to incorporate a fluorescein tag in a DNA molecule. It can be used for the direct enzymatic labeling of DNA/cDNA, e.g. by nick translation, random priming, polymerase chain reaction (PCR), and 3'-end labeling. The resulting fluorescein-labeled DNA/cDNA probes are ideally suited for fluorescence hybridization applications such as FISH or microarray-based gene expression profiling. The linker between fluorescein tag and guanosine has been optimized for the optimal substrate incorporation and labeling efficiency. The labeled products may be detected directly via fluorescence or indirectly by combining with anti-fluorescein HRP or AP conjugates.
Chemical structure for Fluorescein-12-dGTP *1 mM in Tris Buffer (pH 7.5)* .
Chemical structure for Fluorescein-12-dGTP *1 mM in Tris Buffer (pH 7.5)* .
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
1703725 nmoles
Price
 
Physical properties
Molecular weight1030.72
SolventWater
Spectral properties
Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
, 
0.95
2
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 21, 2025