Name: Fluorescein-5-maleimide *CAS 75350-46-8*
Brand: AAT Bioquest
SKU: 130
Price: 148 USD
Availability: InStock

Fluorescein-5-maleimide

CAS 75350-46-8

Maleimides are among the most frequently used reagents for thiol modification. In most proteins, the site of reaction is at cysteine residues that either are intrinsically present or resulted from reduction of cystines. Unlike iodoacetamides, maleimides do not react with histidine and methionine under physiological conditions. Fluorescein-5-maleimide is one of the most popular fluorescent dyes for thiol modifications of proteins along with 5-TMRIA.

Catalog	Size	Price	Quantity
130	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein-5-maleimide *CAS 75350-46-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	233.995 µL	1.17 mL	2.34 mL	11.7 mL	23.399 mL
5 mM	46.799 µL	233.995 µL	467.99 µL	2.34 mL	4.68 mL
10 mM	23.399 µL	116.997 µL	233.995 µL	1.17 mL	2.34 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	427.36 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Multifunctional superparamagnetic nanoparticles conjugated with fluorescein-labeled designed ankyrin repeat protein as an efficient HER2-targeted probe in breast cancer

Authors:

Li, Dong-Li and Tan, Jian-Er and Tian, Ying and Huang, Shun and Sun, Peng-Hui and Wang, Meng and Han, Yan-Jiang and Li, Hong-Sheng and Wu, Hu-Bing and Zhang, Xing-Mei and others, undefined

Journal:

Biomaterials (2017)

Etablierung von zellbasierten Assays zur Identifizierung von Inhibitoren des Chemokins CXCL8

Authors:

J{\"o}st, Marina

Untersuchungsmethoden f{\"u}r die chemokininduzierte Migration von Leukozyten

Authors:

Rink, Ina

Targeted Mesoporous Silica Nanoparticles Delivering Arsenic Trioxide with Environment Sensitive Drug Release for Effective Treatment of Triple Negative Breast Cancer

Authors:

Wu, Xiaohui and Han, Zheng and Schur, Rebecca M and Lu, Zheng-Rong

Journal:

ACS Biomaterials Science & Engineering (2016): 501--507

References

View all 87 references: Citation Explorer

Bovine factor B: cloning, expression, and characterization

Authors:

Belogrudov GI., undefined

Journal:

Arch Biochem Biophys (2006): 68

Substrates induce conformational changes in human anion exchanger 1 (hAE1) as observed by fluorescence resonance energy transfer

Authors:

Pal P, Lebedev D, Salim S, Knauf PA.

Journal:

Biochemistry (2006): 6279

Fluorometric measurements of intermolecular distances between the alpha and beta subunits of the Na+/K+-ATPase

Authors:

Dempski RE, Hartung K, Friedrich T, Bamberg E.

Journal:

J Biol Chem. (2006)

Loop VIII/IX of the Na+-citrate transporter CitS of Klebsiella pneumoniae folds into an amphipathic surface helix

Authors:

Sobczak I, Lolkema JS.

Journal:

Biochemistry (2005): 5461

Essential role for Pro21 in phospholamban for optimal inhibition of the Ca-ATPase

Authors:

Li J, Boschek CB, Xiong Y, Sacksteder CA, Squier TC, Bigelow DJ.

Journal:

Biochemistry (2005): 16181

Physical properties

Molecular weight	427.36
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	75350-46-8

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