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Fluorescein *CAS 2321-07-5*

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Physical properties
Molecular weight332.31
Spectral properties
Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952
Storage, safety and handling
Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Fluoresceins
Molecular weight
Absorbance (nm)
Correction Factor (260 nm)
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
0.79001, 0.952
Fluorescein is a fluorophore widely used in microscopy, forensics and serology to detect latent blood stains. Fluorescein has an absorption maximum at 490 nm and emission maximum of 514 nm (in water). It has a pKa at 6.4 and multiple ionization equilibria. This leads to pH dependent absorption and emission over the range of 5 to 9. It has an isoabsorptive point at 460 nm.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein *CAS 2321-07-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM300.924 µL1.505 mL3.009 mL15.046 mL30.092 mL
5 mM60.185 µL300.924 µL601.848 µL3.009 mL6.018 mL
10 mM30.092 µL150.462 µL300.924 µL1.505 mL3.009 mL

Molarity calculator

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Spectral properties

Absorbance (nm)487
Correction Factor (260 nm)0.32
Correction Factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)800001
Excitation (nm)498
Emission (nm)517
Quantum yield0.79001, 0.952



View all 91 references: Citation Explorer
Lipoxin A inhibits porphyromonas gingivalis-induced aggregation and reactive oxygen species production by modulating neutrophil-platelet interaction and CD11b expression
Authors: Borgeson E, Lonn J, Bergstrom I, Brodin VP, Ramstrom S, Nayeri F, Sarndahl E, Bengtsson T.
Journal: Infect Immun (2011): 1489
Role of hyaluronan and CD44 in reactive oxygen species-induced mucus hypersecretion
Authors: Yu H, Li Q, Zhou X, Kolosov VP, Perelman JM.
Journal: Mol Cell Biochem (2011): 65
Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells
Authors: Hossain MZ, Kleve MG.
Journal: Int J Nanomedicine (2011): 1475
Effect of glucocorticoid on production of reactive oxygen species in bone microvascular endothelial cells
Authors: Yang Y, Lou J, Li Z, Sun W, Wang B, Jia Y.
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533
Regulation of cyclooxygenase-2 and cytosolic phospholipase A2 gene expression by lipopolysaccharide through the RNA-binding protein HuR: involvement of NADPH oxidase, reactive oxygen species and mitogen-activated protein kinases
Authors: Lin WN, Lin CC, Cheng HY, Yang CM.
Journal: Br J Pharmacol (2011): 1691
Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951
Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266