Fluorescein *CAS 2321-07-5*

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Chemical structure for Fluorescein *CAS 2321-07-5*
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Overview

Ex/Em (nm)497/516
MW332.31
CAS #2321-07-5
SolventDMSO
Storage Freeze (<-15 °C)
Minimize light exposure
Category Chemical Building Blocks and Precursors
Fluorescein is a fluorophore widely used in microscopy, forensics and serology to detect latent blood stains. Fluorescein has an absorption maximum at 490 nm and emission maximum of 514 nm (in water). It has a pKa at 6.4 and multiple ionization equilibria. This leads to pH dependent absorption and emission over the range of 5 to 9. It has an isoabsorptive point at 460 nm.




Calculators
Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein *CAS 2321-07-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.



Molarity calculator

Table 2. Enter any two values (mass, volume, concentration) to calculate the third.

Mass Molecular weight Volume Concentration Moles
/ = x =
 






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