Fluorescein *CAS 2321-07-5*
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Additional ordering information
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| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 332.31 |
| Solvent | DMSO |
Spectral properties
| Absorbance (nm) | 487 |
| Correction Factor (260 nm) | 0.32 |
| Correction Factor (280 nm) | 0.35 |
| Extinction coefficient (cm -1 M -1) | 800001 |
| Excitation (nm) | 498 |
| Emission (nm) | 517 |
| Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
| Certificate of Origin | Download PDF |
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Fluoresceins
CAS 2321-07-5 | Molecular weight 332.31 | Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
Fluorescein is a fluorophore widely used in microscopy, forensics and serology to detect latent blood stains. Fluorescein has an absorption maximum at 490 nm and emission maximum of 514 nm (in water). It has a pKa at 6.4 and multiple ionization equilibria. This leads to pH dependent absorption and emission over the range of 5 to 9. It has an isoabsorptive point at 460 nm.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein *CAS 2321-07-5* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 300.924 µL | 1.505 mL | 3.009 mL | 15.046 mL | 30.092 mL |
| 5 mM | 60.185 µL | 300.924 µL | 601.848 µL | 3.009 mL | 6.018 mL |
| 10 mM | 30.092 µL | 150.462 µL | 300.924 µL | 1.505 mL | 3.009 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 487 |
| Correction Factor (260 nm) | 0.32 |
| Correction Factor (280 nm) | 0.35 |
| Extinction coefficient (cm -1 M -1) | 800001 |
| Excitation (nm) | 498 |
| Emission (nm) | 517 |
| Quantum yield | 0.79001, 0.952 |
Images
Figure 1. Chemical structure for Fluorescein *CAS 2321-07-5*
References
View all 91 references: Citation Explorer
Lipoxin A inhibits porphyromonas gingivalis-induced aggregation and reactive oxygen species production by modulating neutrophil-platelet interaction and CD11b expression
Authors: Borgeson E, Lonn J, Bergstrom I, Brodin VP, Ramstrom S, Nayeri F, Sarndahl E, Bengtsson T.
Journal: Infect Immun (2011): 1489
Authors: Borgeson E, Lonn J, Bergstrom I, Brodin VP, Ramstrom S, Nayeri F, Sarndahl E, Bengtsson T.
Journal: Infect Immun (2011): 1489
Role of hyaluronan and CD44 in reactive oxygen species-induced mucus hypersecretion
Authors: Yu H, Li Q, Zhou X, Kolosov VP, Perelman JM.
Journal: Mol Cell Biochem (2011): 65
Authors: Yu H, Li Q, Zhou X, Kolosov VP, Perelman JM.
Journal: Mol Cell Biochem (2011): 65
Nickel nanowires induced and reactive oxygen species mediated apoptosis in human pancreatic adenocarcinoma cells
Authors: Hossain MZ, Kleve MG.
Journal: Int J Nanomedicine (2011): 1475
Authors: Hossain MZ, Kleve MG.
Journal: Int J Nanomedicine (2011): 1475
Effect of glucocorticoid on production of reactive oxygen species in bone microvascular endothelial cells
Authors: Yang Y, Lou J, Li Z, Sun W, Wang B, Jia Y.
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533
Authors: Yang Y, Lou J, Li Z, Sun W, Wang B, Jia Y.
Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533
Regulation of cyclooxygenase-2 and cytosolic phospholipase A2 gene expression by lipopolysaccharide through the RNA-binding protein HuR: involvement of NADPH oxidase, reactive oxygen species and mitogen-activated protein kinases
Authors: Lin WN, Lin CC, Cheng HY, Yang CM.
Journal: Br J Pharmacol (2011): 1691
Authors: Lin WN, Lin CC, Cheng HY, Yang CM.
Journal: Br J Pharmacol (2011): 1691
Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951
Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262
The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712
Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702
Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266