Fluorescein DHPE

Fluorescein DHPE is a phospholipid that is labeled on the head group with the bright, green-fluorescent fluorescein dye. Because of fluorescein's photolability, fluorescein DHPE is a useful reagent for measuring lateral diffusion in membranes using fluorescence photobleaching recovery methods. Fluorescein DHPE has also been used in conjunction with polyclonal anti-fluorescein dye antibody to prepare colloidal gold probes for SPT diffusion measurements in supported phospholipid bilayers and in keratocyte plasma membranes.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23304	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein DHPE to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	84.563 µL	422.815 µL	845.63 µL	4.228 mL	8.456 mL
5 mM	16.913 µL	84.563 µL	169.126 µL	845.63 µL	1.691 mL
10 mM	8.456 µL	42.282 µL	84.563 µL	422.815 µL	845.63 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1182.55 g/mol	=	mL	x	mM	=	mmol

Citations

View all 5 citations: Citation Explorer

Highly Robust Multilamellar Lipid Vesicles Generated through Intervesicular Self-assembly Mediated by Hydrolyzed Collagen Peptides

Authors:

Koo, Bon Il and Rahman, Rafia Tasnim and Jang, Jihui and Lee, Dong Jae and Lee, Jun Bae and Nam, Yoon Sung

Activation of Nedd4L Ubiquitin Ligase by FCHO2-generated Membrane Curvature

Authors:

Sakamoto, Yasuhisa and Uezu, Akiyoshi and Kikuchi, Koji and Suetsugu, Shiro and Nakanishi, Hiroyuki

Journal:

bioRxiv (2022)

DNA mechanotechnology reveals that integrin receptors apply pN forces in podosomes on fluid substrates

Authors:

Glazier, Roxanne and Brockman, Joshua M and Bartle, Emily and Mattheyses, Alexa L and Destaing, Olivier and Salaita, Khalid

Journal:

Nature Communications (2019): 1--13

Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis

Authors:

Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira

Journal:

Biomaterials (2018): 74--85

Development of PEGylated aspartic acid-modified liposome as a bone-targeting carrier for the delivery of paclitaxel and treatment of bone metastasis

Authors:

Yamashita, Shugo and Katsumi, Hidemasa and Hibino, Nozomi and Isobe, Yugo and Yagi, Yumiko and Tanaka, Yuka and Yamada, Saki and Naito, Chihiro and Yamamoto, Akira

Journal:

Biomaterials (2017)

References

View all 27 references: Citation Explorer

Permeabilization assay for antimicrobial peptides based on pore-spanning lipid membranes on nanoporous alumina

Authors:

Neubacher H, Mey I, Carnarius C, Lazzara TD, Steinem C.

Journal:

Langmuir (2014): 4767

Membrane order parameters for interdigitated lipid bilayers measured via polarized total-internal-reflection fluorescence microscopy

Authors:

Ngo AT, Jakubek ZJ, Lu Z, Joos B, Morris CE, Johnston LJ.

Journal:

Biochim Biophys Acta (2014): 2861

Mesoporous gold and palladium nanoleaves from liquid-liquid interface: enhanced catalytic activity of the palladium analogue toward hydrazine-assisted room-temperature 4-nitrophenol reduction

Authors:

Dutta S, Sarkar S, Ray C, Roy A, Sahoo R, Pal T.

Journal:

ACS Appl Mater Interfaces (2014): 9134

Phase segregation of polymerizable lipids to construct filters for separating lipid-membrane-embedded species

Authors:

Hu SK, Chen YM, Chao L.

Journal:

Biomicrofluidics (2014): 52005

Lipid reassembly in asymmetric Langmuir-Blodgett/Langmuir-Schaeffer bilayers

Authors:

Yuan J, Hao C, Chen M, Berini P, Zou S.

Journal:

Langmuir (2013): 221

Physical properties

Molecular weight	1182.55
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H301, H311, H331
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R23, R24, R25
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 16, 2025