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Fluorescein dicaprylate
Fluorescein dioctanoate
Fluorescein dicaprylate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dicaprylate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. Fluorescein dicaprylate may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. Fluorescein dicaprylate may stain Salmonella in green fluorescence. It is a fluorogenic substrate for C8 esterase that generates green fluorescence upon esterase cleavage.
Chemical structure for Fluorescein dicaprylate [Fluorescein dioctanoate]
Chemical structure for Fluorescein dicaprylate [Fluorescein dioctanoate]
CatalogSize
Price
Quantity
22019100 mg
Price
 
Physical properties

Molecular weight584.70
SolventDMSO
Spectral properties

Absorbance (nm)487
Correction factor (260 nm)0.32
Correction factor (280 nm)0.35
Extinction coefficient (cm -1 M -1)
80000
1
Excitation (nm)498
Emission (nm)517
Quantum yield
0.7900
1
0.95
2
Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
CAS19722-86-2
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Page updated on October 12, 2025