Name: Fluorescein dicaprylate [Fluorescein dioctanoate]
Brand: AAT Bioquest
SKU: 22019
Price: 115 USD
Availability: InStock

Fluorescein dicaprylate

Fluorescein dioctanoate

Fluorescein dicaprylate is a non-fluorescent hydrophobic fluorescein derivative that can pass through the cell membrane whereupon intracellular esterases hydrolyze the dicaprylate group producing the highly fluorescent product fluorescein. The fluorescein molecules accumulate in cells that possess intact membranes so the green fluorescence can be used as a marker of cell viability. Cells that do not possess an intact cell membrane or an active metabolism may not accumulate the fluorescent product and therefore do not exhibit green fluorescence. Fluorescein dicaprylate may be used in combination with PI staining as the non-viable cells take up the PI and stain dead cells red whereas viable cells do not take up the PI and should only stain green. Fluorescein dicaprylate may stain Salmonella in green fluorescence. It is a fluorogenic substrate for C8 esterase that generates green fluorescence upon esterase cleavage.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22019	100 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein dicaprylate [Fluorescein dioctanoate] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	171.028 µL	855.139 µL	1.71 mL	8.551 mL	17.103 mL
5 mM	34.206 µL	171.028 µL	342.056 µL	1.71 mL	3.421 mL
10 mM	17.103 µL	85.514 µL	171.028 µL	855.139 µL	1.71 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	584.7 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel

Authors:

Xu, Yufan and Wang, Xiaohong

Journal:

Biotechnology and bioengineering (2015): 1683--1695

Overexpression of the CaTIP1-1 pepper gene in tobacco enhances resistance to osmotic stresses

Authors:

Yin, Yan-Xu and Wang, Shu-Bin and Xiao, Huai-Juan and Zhang, Huai-Xia and Zhang, Zhen and Jing, Hua and Zhang, Ying-Li and Chen, Ru-Gang and Gong, Zhen-Hui

Journal:

International journal of molecular sciences (2014): 20101--20116

Identification of VAR2CSA domain-specific inhibitory antibodies of the Plasmodium falciparum erythrocyte membrane protein 1 using a novel flow cytometry assay

Authors:

Obiakor, Harold and Avril, Marion and MacDonald, Nicholas J and Srinivasan, Prakash and Reiter, Karine and Anderson, Charles and Holmes, Kevin L and Fried, Michal and Duffy, Patrick E and Smith, Joseph D and others, undefined

Journal:

Clinical and Vaccine Immunology (2013): 433--442

Aging enhances maceration-induced ultrastructural alteration of the epidermis and impairment of skin barrier function

Authors:

Minematsu, Takeo and Yamamoto, Yuko and Nagase, Takashi and Naito, Ayumi and Takehara, Kimie and Iizaka, Shinji and Komagata, Kazunori and Huang, Lijuan and Nakagami, Gojiro and Akase, Tomoko and others, undefined

Journal:

Journal of dermatological science (2011): 160--168

References

View all 68 references: Citation Explorer

Novel method for cell debris removal in the flow cytometric cell cycle analysis using carboxy-fluorescein diacetate succinimidyl ester

Authors:

Terho P, Lassila O.

Journal:

Cytometry A (2006): 552

Modification of the fluorescein diacetate assay for screening of antifungal agents against Candida albicans: comparison with the NCCLS methods

Authors:

Brouwer N, Kohen J, Jamie J, Vemulpad S.

Journal:

J Microbiol Methods (2006): 234

Optimisation of the fluorescein diacetate antibacterial assay

Authors:

Wan, undefined and y S, Brouwer N, Liu Q, Mahon A, Cork S, Karuso P, Vemulpad S, Jamie J.

Journal:

J Microbiol Methods (2005): 21

A three-dimensional flow control concept for single-cell experiments on a microchip. 2. Fluorescein diacetate metabolism and calcium mobilization in a single yeast cell as stimulated by glucose and pH changes

Authors:

Peng XY, Li PC.

Journal:

Anal Chem (2004): 5282

Effect of immunosuppressants on T-cell subsets observed in vivo using carboxy-fluorescein diacetate succinimidyl ester labeling

Authors:

Hu H, Dong Y, Feng P, Fechner J, Hamawy M, Knechtle SJ.

Journal:

Transplantation (2003): 1075

Physical properties

Molecular weight	584.70
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	19722-86-2

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Page updated on October 15, 2025