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Fluorescein dicaprylate WS1

Fluorescein dicaprylate WS1 is a non-fluorescent water-soluble fluorescein derivative. Esterases hydrolyze the dicaprylate group producing the highly fluorescent product fluorescein. This water-soluble fluorescein dicaprylate may stain Salmonella in green fluorescence. It is a fluorogenic substrate for C8 esterase that generates green fluorescence upon esterase cleavage. Compared to the fluorescein dicaprylate esterase substrate, this fluorescein dicaprylate WS1 is much more water-soluble while fluorescein dicaprylate precipitates in aqueous solutions, making it hard to be used in continuous assays.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Fluorescein dicaprylate WS1 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM120.632 µL603.158 µL1.206 mL6.032 mL12.063 mL
5 mM24.126 µL120.632 µL241.263 µL1.206 mL2.413 mL
10 mM12.063 µL60.316 µL120.632 µL603.158 µL1.206 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

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Spectrum

Citations

View all 4 citations: Citation Explorer
Fluid and cell behaviors along a 3D printed alginate/gelatin/fibrin channel
Authors: Xu, Yufan and Wang, Xiaohong
Journal: Biotechnology and bioengineering (2015): 1683--1695
Overexpression of the CaTIP1-1 pepper gene in tobacco enhances resistance to osmotic stresses
Authors: Yin, Yan-Xu and Wang, Shu-Bin and Xiao, Huai-Juan and Zhang, Huai-Xia and Zhang, Zhen and Jing, Hua and Zhang, Ying-Li and Chen, Ru-Gang and Gong, Zhen-Hui
Journal: International journal of molecular sciences (2014): 20101--20116
Identification of VAR2CSA domain-specific inhibitory antibodies of the Plasmodium falciparum erythrocyte membrane protein 1 using a novel flow cytometry assay
Authors: Obiakor, Harold and Avril, Marion and MacDonald, Nicholas J and Srinivasan, Prakash and Reiter, Karine and Anderson, Charles and Holmes, Kevin L and Fried, Michal and Duffy, Patrick E and Smith, Joseph D and others, undefined
Journal: Clinical and Vaccine Immunology (2013): 433--442
Aging enhances maceration-induced ultrastructural alteration of the epidermis and impairment of skin barrier function
Authors: Minematsu, Takeo and Yamamoto, Yuko and Nagase, Takashi and Naito, Ayumi and Takehara, Kimie and Iizaka, Shinji and Komagata, Kazunori and Huang, Lijuan and Nakagami, Gojiro and Akase, Tomoko and others, undefined
Journal: Journal of dermatological science (2011): 160--168

References

View all 68 references: Citation Explorer
Novel method for cell debris removal in the flow cytometric cell cycle analysis using carboxy-fluorescein diacetate succinimidyl ester
Authors: Terho P, Lassila O.
Journal: Cytometry A (2006): 552
Modification of the fluorescein diacetate assay for screening of antifungal agents against Candida albicans: comparison with the NCCLS methods
Authors: Brouwer N, Kohen J, Jamie J, Vemulpad S.
Journal: J Microbiol Methods (2006): 234
Optimisation of the fluorescein diacetate antibacterial assay
Authors: Wan, undefined and y S, Brouwer N, Liu Q, Mahon A, Cork S, Karuso P, Vemulpad S, Jamie J.
Journal: J Microbiol Methods (2005): 21
A three-dimensional flow control concept for single-cell experiments on a microchip. 2. Fluorescein diacetate metabolism and calcium mobilization in a single yeast cell as stimulated by glucose and pH changes
Authors: Peng XY, Li PC.
Journal: Anal Chem (2004): 5282
Effect of immunosuppressants on T-cell subsets observed in vivo using carboxy-fluorescein diacetate succinimidyl ester labeling
Authors: Hu H, Dong Y, Feng P, Fechner J, Hamawy M, Knechtle SJ.
Journal: Transplantation (2003): 1075
Page updated on October 14, 2024

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Catalog Number22018
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Physical properties

Molecular weight

828.97

Solvent

DMSO

Spectral properties

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm -1 M -1)

800001

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.79001, 0.952

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200
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