Name: Fluorescein, disodium salt *CAS 518-47-8*
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Fluorescein, disodium salt

CAS 518-47-8

Fluorescein, disodium salt is water-soluble version of fluorescein (see product #1). Fluorescein is a fluorophore widely used in microscopy, forensics and serology to detect latent blood stains. Fluorescein has an absorption maximum at 490 nm and emission maximum of 514 nm (in water). It has a pKa at 6.4 and multiple ionization equilibria. This leads to pH dependent absorption and emission over the range of 5 to 9. It has an isoabsorptive point at 460 nm.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2	100 mg	Price

Citations

View all 3 citations: Citation Explorer

A DUF21 domain-containing protein regulates plant dwarfing in watermelon

Authors:

Sun, Piaoyun and Zhao, Hongjiao and Cao, Lihong and Zhang, Tian and Zhang, Helong and Yang, Tongwen and Zhao, Bosi and Jiang, Yanxin and Dong, Junyang and Chen, Tianrui and others,

Journal:

Plant Physiology (2024): kiae486

Measuring Diffusion Through Carbon Nanotube Arrays with Fluorescent Spectroscopy

Authors:

Machold, Michael

Fluorescence Guided Surgery

Authors:

Stewart, Hazel L and Birch, David JS

Journal:

Methods and Applications in Fluorescence (2021)

References

View all 91 references: Citation Explorer

Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm

Authors:

Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.

Journal:

Theriogenology (2011): 951

Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells

Authors:

Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.

Journal:

Acta Pharmacol Sin (2011): 1266

Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice

Authors:

Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.

Journal:

Pharm Res (2011): 702

The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin

Authors:

Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.

Journal:

Toxicol In Vitro (2011): 1712

Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging

Authors:

Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.

Journal:

Chemistry (2011): 5262

Physical properties

Molecular weight	376.27
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	518-47-8

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