Fluorescein, disodium salt CAS 518-47-8

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Physical properties

Molecular weight	376.27
Solvent	Water

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluorescein, disodium salt *CAS 518-47-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	265.767 µL	1.329 mL	2.658 mL	13.288 mL	26.577 mL
5 mM	53.153 µL	265.767 µL	531.533 µL	2.658 mL	5.315 mL
10 mM	26.577 µL	132.883 µL	265.767 µL	1.329 mL	2.658 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
MUP, disodium salt [4-Methylumbelliferyl phosphate, disodium salt] CAS 22919-26-2	360	448	18000	0.1

Images

Figure 1. Chemical structure for Fluorescein, disodium salt *CAS 518-47-8*

Citations

View all 2 citations: Citation Explorer

Measuring Diffusion Through Carbon Nanotube Arrays with Fluorescent Spectroscopy
Authors: Machold, Michael
Journal: (2023)

Fluorescence Guided Surgery
Authors: Stewart, Hazel L and Birch, David JS
Journal: Methods and Applications in Fluorescence (2021)

References

View all 91 references: Citation Explorer

Lipoxin A inhibits porphyromonas gingivalis-induced aggregation and reactive oxygen species production by modulating neutrophil-platelet interaction and CD11b expression
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Journal: Infect Immun (2011): 1489

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Journal: Mol Cell Biochem (2011): 65

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Journal: Int J Nanomedicine (2011): 1475

Effect of glucocorticoid on production of reactive oxygen species in bone microvascular endothelial cells
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Journal: Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi (2011): 533

Regulation of cyclooxygenase-2 and cytosolic phospholipase A2 gene expression by lipopolysaccharide through the RNA-binding protein HuR: involvement of NADPH oxidase, reactive oxygen species and mitogen-activated protein kinases
Authors: Lin WN, Lin CC, Cheng HY, Yang CM.
Journal: Br J Pharmacol (2011): 1691

Effects of hypothermic storage on intracellular calcium, reactive oxygen species formation, mitochondrial function, motility, and plasma membrane integrity in striped bass (Morone saxatilis) sperm
Authors: Guthrie HD, Welch GR, Theisen DD, Woods LC, 3rd.
Journal: Theriogenology (2011): 951

Coenzyme Q functionalized CdTe/ZnS quantum dots for reactive oxygen species (ROS) imaging
Authors: Qin LX, Ma W, Li DW, Li Y, Chen X, Kraatz HB, James TD, Long YT.
Journal: Chemistry (2011): 5262

The role of reactive oxygen species in WP 631-induced death of human ovarian cancer cells: a comparison with the effect of doxorubicin
Authors: Rogalska A, Gajek A, Szwed M, Jozwiak Z, Marczak A.
Journal: Toxicol In Vitro (2011): 1712

Positive correlation between the generation of reactive oxygen species and activation/reactivation of transgene expression after hydrodynamic injections into mice
Authors: Takiguchi N, Takahashi Y, Nishikawa M, Matsui Y, Fukuhara Y, Oushiki D, Kiyose K, Hanaoka K, Nagano T, Takakura Y.
Journal: Pharm Res (2011): 702

Reactive oxygen species contribute to oridonin-induced apoptosis and autophagy in human cervical carcinoma HeLa cells
Authors: Zhang YH, Wu YL, Tashiro S, Onodera S, Ikejima T.
Journal: Acta Pharmacol Sin (2011): 1266