Fluorescein-dT CPG

Fluorescein CPG has traditionally been used to add the fluorescein label at the 3'-terminus. 3'-(6-FAM) CPG allows effective blockage of the 3'-terminus from polymerase extension as well as exonuclease digestion. Fluorescein-dT CPG allows both of these enzymatic activities to proceed. Normal cleavage and deprotection with ammonium hydroxide readily generate the fluorescein labelled oligos.

Protocol

COA

Catalog	Size	Price	Quantity
6205	100 mg	Price
6206	1 g	Price

References

View all 2 references: Citation Explorer

Label-Free Homogeneous microRNA Detection in Cell Culture Medium Based on Graphene Oxide and Specific Fluorescence Quenching.

Authors:

Nitu, Florentin R and Savu, Lorand and Muraru, Sorin and Stoian, Ioan and Ionită, Mariana

Journal:

Nanomaterials (Basel, Switzerland) (2021)

Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique.

Authors:

Numata, Munenori and Koumoto, Kazuya and Mizu, Masami and Sakurai, Kazuo and Shinkai, Seiji

Journal:

Organic & biomolecular chemistry (2005): 2255-61

Physical properties

Solvent

MeCN

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 6205)
Safety Data Sheet (Catalog 6206)
Certificate of Analysis

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Page updated on November 4, 2025