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Fluorescein-dT CPG
Ordering information
| Price | |
| Catalog Number | |
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Solvent | MeCN |
Spectral properties
| Absorbance (nm) | 487 |
| Correction Factor (260 nm) | 0.32 |
| Correction Factor (280 nm) | 0.35 |
| Extinction coefficient (cm -1 M -1) | 800001 |
| Excitation (nm) | 498 |
| Emission (nm) | 517 |
| Quantum yield | 0.79001, 0.952 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Fluoresceins
Absorbance (nm) 487 | Correction Factor (260 nm) 0.32 | Correction Factor (280 nm) 0.35 | Extinction coefficient (cm -1 M -1) 800001 | Excitation (nm) 498 | Emission (nm) 517 | Quantum yield 0.79001, 0.952 |
Fluorescein CPG has traditionally been used to add the fluorescein label at the 3'-terminus. 3'-(6-FAM) CPG allows effective blockage of the 3'-terminus from polymerase extension as well as exonuclease digestion. Fluorescein-dT CPG allows both of these enzymatic activities to proceed. Normal cleavage and deprotection with ammonium hydroxide readily generate the fluorescein labelled oligos.
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Absorbance (nm) | 487 |
| Correction Factor (260 nm) | 0.32 |
| Correction Factor (280 nm) | 0.35 |
| Extinction coefficient (cm -1 M -1) | 800001 |
| Excitation (nm) | 498 |
| Emission (nm) | 517 |
| Quantum yield | 0.79001, 0.952 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield | Correction Factor (260 nm) | Correction Factor (280 nm) |
| 3'-Fluorescein CPG *1000 Å* | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
| Fluorescein-dT Phosphoramidite | 498 | 517 | 800001 | 0.79001, 0.952 | 0.32 | 0.35 |
Images
Figure 1. Chemical structure for Fluorescein-dT CPG.
References
View all 2 references: Citation Explorer
Label-Free Homogeneous microRNA Detection in Cell Culture Medium Based on Graphene Oxide and Specific Fluorescence Quenching.
Authors: Nitu, Florentin R and Savu, Lorand and Muraru, Sorin and Stoian, Ioan and Ionită, Mariana
Journal: Nanomaterials (Basel, Switzerland) (2021)
Authors: Nitu, Florentin R and Savu, Lorand and Muraru, Sorin and Stoian, Ioan and Ionită, Mariana
Journal: Nanomaterials (Basel, Switzerland) (2021)
Parallel vs. anti-parallel orientation in a curdlan/oligo(dA) complex as estimated by a FRET technique.
Authors: Numata, Munenori and Koumoto, Kazuya and Mizu, Masami and Sakurai, Kazuo and Shinkai, Seiji
Journal: Organic & biomolecular chemistry (2005): 2255-61
Authors: Numata, Munenori and Koumoto, Kazuya and Mizu, Masami and Sakurai, Kazuo and Shinkai, Seiji
Journal: Organic & biomolecular chemistry (2005): 2255-61
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