Fluorescein-dT Phosphoramidite

Fluorescein-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the fluorescein tag to a desired location of an oligo sequence. It is complimentary to the commonly used 6-FAM phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. AAT Bioquest also offer HEX-dT phosphoramidite, TET-dT phosphoramidite, biotin-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.

Protocol

Catalog	Size	Price	Quantity
6200	50 umoles	Price
6201	100 umoles	Price

Calculators

Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of Fluorescein-dT Phosphoramidite to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	70.147 µL	350.734 µL	701.469 µL	3.507 mL	7.015 mL
5 mM	14.029 µL	70.147 µL	140.294 µL	701.469 µL	1.403 mL
10 mM	7.015 µL	35.073 µL	70.147 µL	350.734 µL	701.469 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1425.58 g/mol	=	mL	x	mM	=	mmol

References

View all 4 references: Citation Explorer

A Chiral-Nanoassemblies-Enabled Strategy for Simultaneously Profiling Surface Glycoprotein and MicroRNA in Living Cells.

Authors:

Ma, Wei and Sun, Maozhong and Fu, Pan and Li, Si and Xu, Liguang and Kuang, Hua and Xu, Chuanlai

Journal:

Advanced materials (Deerfield Beach, Fla.) (2017)

Sorting the unique chirality, right handed single wall carbon nanotubes via the dye modified ssDNA.

Authors:

Liu, Ru and Chen, Zhong and Zhao, Feng and Chang, Xueling and Zu, Yan and Gao, Xueyun

Journal:

Journal of nanoscience and nanotechnology (2011): 7587-92

Identification of unexpected modifications of fluorescein-labeled oligodeoxynucleotides by nuclease P1 digestion and mass spectrometric techniques.

Authors:

Wu, H and Skrzypczynski, Z and Cornwell, M J and Aboleneen, H

Journal:

Rapid communications in mass spectrometry : RCM (2000): 26-32

Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles.

Authors:

Ramírez-Vick, J E and García, A A and Lee, J

Journal:

Preparative biochemistry & biotechnology (1998): 243-60

Physical properties

Molecular weight	1425.58
Solvent	MeCN

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	289712-99-8

Documents

Protocol
Safety Data Sheet (Catalog 6200)
Safety Data Sheet (Catalog 6201)
Certificate of Analysis
Flyer: Labeled Phosphoramidites

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Page updated on November 23, 2025