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Fluorescein-dT Phosphoramidite

Fluorescein-dT phosphoramidite contains a DMT group to allow quantification of coupling. It can be inserted into a target sequence as a replacement for a dT residue. It provides a valuable tool to add the fluorescein tag to a desired location of an oligo sequence. It is complimentary to the commonly used 6-FAM phosphoramidite that contains no DMT group and can only be added at the 5'-terminus, thus terminating synthesis. AAT Bioquest also offer HEX-dT phosphoramidite, TET-dT phosphoramidite, biotin-dT phosphoramidite, biotin-dT CPG and Fluorescein-dT CPG.

Spectrum

References

View all 4 references: Citation Explorer
A Chiral-Nanoassemblies-Enabled Strategy for Simultaneously Profiling Surface Glycoprotein and MicroRNA in Living Cells.
Authors: Ma, Wei and Sun, Maozhong and Fu, Pan and Li, Si and Xu, Liguang and Kuang, Hua and Xu, Chuanlai
Journal: Advanced materials (Deerfield Beach, Fla.) (2017)
Sorting the unique chirality, right handed single wall carbon nanotubes via the dye modified ssDNA.
Authors: Liu, Ru and Chen, Zhong and Zhao, Feng and Chang, Xueling and Zu, Yan and Gao, Xueyun
Journal: Journal of nanoscience and nanotechnology (2011): 7587-92
Identification of unexpected modifications of fluorescein-labeled oligodeoxynucleotides by nuclease P1 digestion and mass spectrometric techniques.
Authors: Wu, H and Skrzypczynski, Z and Cornwell, M J and Aboleneen, H
Journal: Rapid communications in mass spectrometry : RCM (2000): 26-32
Recovery of an oligonucleotide using silver ions immobilized onto paramagnetic particles.
Authors: Ramírez-Vick, J E and García, A A and Lee, J
Journal: Preparative biochemistry & biotechnology (1998): 243-60
Page updated on October 12, 2024

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Physical properties

Molecular weight

1425.58

Solvent

MeCN

Spectral properties

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm -1 M -1)

800001

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.79001, 0.952

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

289712-99-8
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