Fluoro-Jade®C

Fluoro-Jade® C, like its predecessors Fluoro-Jade and Fluoro-Jade B, will stain all degenerating neurons, regardless of specific insult or mechanism of cell death. Therefore, the patterns of neuronal degeneration seen following exposure to either the glutamate agonist, kainic acid, or the inhibitor of mitochondrial respiration, 3-NPA, were the same for all of the Fluoro-Jade dyes. However, there was a qualitative difference in the staining characteristics of the three fluorochromes. Specifically, Fluoro-Jade C exhibited the greatest signal to background ratio, as well as the highest resolution. This translates to a stain of maximal contrast and affinity for degenerating neurons. This makes it ideal for localizing not only degenerating nerve cell bodies, but also distal dendrites, axons and terminals. The dye is highly resistant to fading and is compatible with virtually all histological processing and staining protocols. Triple labeling can be accomplished by staining degenerating neurons with Fluoro-Jade C, cell nuclei with DAPI and activated astrocytes with GFAP immunofluorescence. Fluoro-Jade® is a registered trademark of Histo-Chem, Inc.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
23062	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Fluoro-Jade®C to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	121.507 µL	607.533 µL	1.215 mL	6.075 mL	12.151 mL
5 mM	24.301 µL	121.507 µL	243.013 µL	1.215 mL	2.43 mL
10 mM	12.151 µL	60.753 µL	121.507 µL	607.533 µL	1.215 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	823 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

T cell infiltration into the brain triggers pulmonary dysfunction in murine Cryptococcus-associated IRIS

Authors:

Kawano, Tasuku and Zhou, Jinyan and Anwar, Shehata and Salah, Haneen and Dayal, Andrea H and Ishikawa, Yuzuki and Boetel, Katelyn and Takahashi, Tomoko and Sharma, Kamal and Inoue, Makoto

Journal:

Nature Communications (2023): 3831

Cervical Vagus Nerve Stimulation Improves Neurologic Outcome After Cardiac Arrest in Mice by Attenuating Oxidative Stress and Excessive Autophagy

Authors:

Duan, Weina and Sun, Qian and Wu, Xiaojing and Xia, Zhongyuan and Warner, David S and Ulloa, Luis and Yang, Wei and Sheng, Huaxin

Journal:

Neuromodulation: Technology at the Neural Interface (2022): 414--423

CircTYW1 serves as a sponge for microRNA-380 in accelerating neurological recovery following spinal cord injury via regulating FGF9

Authors:

Sun, Yanpeng and Zhou, Yingjie and Shi, Xiangqin and Ma, Xiaoran and Peng, Xiaodong and Xie, Yan and Cao, Xiangyang

Journal:

Cell Cycle (2021): 1828--1844

Determinants of optogenetic cortical spreading depolarizations

Authors:

Chung, David Y and Sadeghian, Homa and Qin, Tao and Lule, Sevda and Lee, Hang and Karakaya, Fahri and Goins, Stacy and Oka, Fumiaki and Yaseen, Mohammad A and Houben, Thijs and others,

Journal:

Cerebral Cortex (2019): 1150--1161

Combinatory FK506 and Minocycline Treatment Alleviates Prion-Induced Neurodegenerative Events via Caspase-Mediated MAPK-NRF2 Pathway

Authors:

Shah, Syed Zahid Ali and Zhao, Deming and Taglialatela, Giulio and Hussain, Tariq and Dong, Haodi and Sabir, Naveed and Mangi, Mazhar Hussain and Wu, Wei and Lai, Mengyu and Zhang, Xixi and others, undefined

Journal:

International journal of molecular sciences (2019): 1144

References

View all 38 references: Citation Explorer

Neuronal Damage Using Fluoro-Jade B Histofluorescence and Gliosis in the Gerbil Septum Submitted to Various Durations of Cerebral Ischemia

Authors:

Park CW, Lee JC, Ahn JH, Lee DH, Cho GS, Yan BC, Park JH, Kim IH, Lee HY, Won MH, Cho JH.

Journal:

Cell Mol Neurobiol. (2013)

Neuronal damage in hippocampal subregions induced by various durations of transient cerebral ischemia in gerbils using Fluoro-Jade B histofluorescence

Authors:

Yu DK, Yoo KY, Shin BN, Kim IH, Park JH, Lee CH, Choi JH, Cho YJ, Kang IJ, Kim YM, Won MH.

Journal:

Brain Res (2012): 50

One-step labeling of degenerative neurons in unfixed brain tissue samples using Fluoro-Jade C

Authors:

Gu Q, Schmued LC, Sarkar S, Paule MG, Raymick B.

Journal:

J Neurosci Methods (2012): 40

Neuronal damage using fluoro-jade B histofluorescence and gliosis in the striatum after various durations of transient cerebral ischemia in gerbils

Authors:

Ohk TG, Yoo KY, Park SM, Shin BN, Kim IH, Park JH, Ahn HC, Lee YJ, Kim MJ, Kim TY, Won MH, Cho JH.

Journal:

Neurochem Res (2012): 826

Fluoro jade-C staining in the assessment of brain injury after deep hypothermia circulatory arrest

Authors:

Wang R, Ma WG, Gao GD, Mao QX, Zheng J, Sun LZ, Liu YL.

Journal:

Brain Res (2011): 127

Physical properties

Molecular weight	823
Solvent	Water

Spectral properties

Excitation (nm)	506
Emission (nm)	527

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on July 12, 2023