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FluoroQuest™ Fluorescence Quantum Yield Determination Kit
Optimized for Bioconjugates
When a fluorophore absorbs a photon of light, an energetically excited state is formed. The fate of this species is varied, depending upon the exact nature of the fluorophore and its surroundings, but the end result is deactivation (loss of energy) and return to the ground state. The main deactivation processes which occur are fluorescence (loss of energy by emission of a photon), internal conversion and vibrational relaxation (non-radiative loss of energy as heat to the surroundings), and intersystem crossing to the triplet manifold and subsequent non-radiative deactivation. The fluorescence quantum yield is the ratio of photons absorbed to photons emitted through fluorescence. In other words the quantum yield gives the probability of the excited state being deactivated by fluorescence rather than by another, non-radiative mechanism. The kit provides all the essential components for fluorescence quantum yield determination for biological conjugates. It is optimized for determining the fluorescence quantum yields of fluorescent protein conjugates, peptides, nucleotides and nucleic acids.
<p>Principle of fluorescence. Electrons are excited to a higher energy level by external source. Upon return to their ground state, a set quanta of photons are release proportional to the energy loss by electrons. This release of photons represents the fluorescence emission.The fluorophore's quantum yield is the ratio of its emitted photons to the photons it absorbed.</p>
<p>Principle of fluorescence. Electrons are excited to a higher energy level by external source. Upon return to their ground state, a set quanta of photons are release proportional to the energy loss by electrons. This release of photons represents the fluorescence emission.The fluorophore's quantum yield is the ratio of its emitted photons to the photons it absorbed.</p>
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
201 kit
Price
 
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
UNSPSC12171501
Instrument settings
Spectrofluorometer
ExcitationSee Table 1
EmissionSee Table 1
Fluorescence microplate reader
ExcitationSee Table 1
EmissionSee Table 1
Recommended plateSolid black
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Page updated on September 25, 2025