FluoroQuest™ Mounting Medium with DAPI

HeLa cells were stained with mouse anti-tubulin and then followed with iFluor<sup>TM</sup> 610 goat anti-mouse IgG (H+L). Cells were mounted in FluoroQuest™ Mounting Medium with DAPI (Cat# 20004).

Western corn rootworm midgut morphology after feeding with Vpb4Da2. Vpb4Da2 staining by Alexa-fluor-488 conjugated secondary antibody is in green. Nuclei staining by DAPI is in blue. (A) Longitudinal section of the midgut after 6 h exposure to Vpb4Da2. (B) Cross section after 48 h exposure to Vpb4Da2. (C) Cross section after 96 h exposure to Vpb4Da2. (A) and (B) White arrows indicate blebbing of the epithelial cells. (A), (B) and (C) White arrowheads designate a complete or near complete loss of the apical microvilli layer. (D), (E), and (F) Are untreated controls where black arrowheads indicate the intact apical microvilli layer. Scale bars are all 50 μm.
Source: <b>Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm</b> by
Kouadio JL, Zheng M, Aikins M, Duda D, Duff S, et al. <em>PLOS ONE</em> Dec. 2021.

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
Quotation	Request
International	See distributors
Shipping	Standard overnight for United States, inquire for international

Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Extinction coefficient (cm ^-1 M ^-1)

27000

Excitation (nm)

359

Emission (nm)

457

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This mounting medium is fortified with DAPI which is a counterstain for DNA. For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Images

Figure 1. HeLa cells were stained with mouse anti-tubulin and then followed with iFluor^TM 610 goat anti-mouse IgG (H+L). Cells were mounted in FluoroQuest™ Mounting Medium with DAPI (Cat# 20004).

Figure 2. Chemical structure for FluoroQuest™ Mounting Medium with DAPI

Figure 3. Western corn rootworm midgut morphology after feeding with Vpb4Da2. Vpb4Da2 staining by Alexa-fluor-488 conjugated secondary antibody is in green. Nuclei staining by DAPI is in blue. (A) Longitudinal section of the midgut after 6 h exposure to Vpb4Da2. (B) Cross section after 48 h exposure to Vpb4Da2. (C) Cross section after 96 h exposure to Vpb4Da2. (A) and (B) White arrows indicate blebbing of the epithelial cells. (A), (B) and (C) White arrowheads designate a complete or near complete loss of the apical microvilli layer. (D), (E), and (F) Are untreated controls where black arrowheads indicate the intact apical microvilli layer. Scale bars are all 50 μm. Source: Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm by Kouadio JL, Zheng M, Aikins M, Duda D, Duff S, et al. PLOS ONE Dec. 2021.

Citations

View all 8 citations: Citation Explorer

Structural and functional characterization of Mpp75Aa1. 1, a putative beta-pore forming protein from Brevibacillus laterosporus active against the western corn rootworm
Authors: Kouadio, Jean-Louis and Duff, Stephen and Aikins, Michael and Zheng, Meiying and Rydel, Timothy and Chen, Danqi and Bretsnyder, Eric and Xia, Chunsheng and Zhang, Jun and Milligan, Jason and others,
Journal: PloS one (2021): e0258052

Acute melatonin administration improves exercise tolerance and the metabolic recovery after exhaustive effort
Authors: Faria, Vin{\'\i}cius Silva and Pejon, Taciane Maria Melges and Gobatto, Claudio Alexandre and de Araujo, Gustavo Gomes and Cornachione, Anabelle Silva and Beck, Wladimir Rafael
Journal: Scientific Reports (2021): 1--11

Simultaneous hyperbaric oxygen therapy during systemic chemotherapy reverses chemotherapy-induced peripheral neuropathy by inhibiting TLR4 and TRPV1 activation in the central and peripheral nervous system
Authors: Chou, Ping-Ruey and Lu, Ching-Yeh and Kan, Jung-Yu and Wang, Shih-Hung and Lo, Jing-Jou and Huang, Shu-Hung and Wu, Sheng-Hua
Journal: Supportive Care in Cancer (2021): 1--10

Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm
Authors: Kouadio, Jean-Louis and Zheng, Meiying and Aikins, Michael and Duda, David and Duff, Stephen and Chen, Danqi and Zhang, Jun and Milligan, Jason and Taylor, Christina and Mamanella, Patricia and others,
Journal: PloS one (2021): e0260532

Comparison between the therapeutic effects of differentiated and undifferentiated Wharton's jelly mesenchymal stem cells in rats with streptozotocin-induced diabetes
Authors: Hsiao, Chen-Yuan and Chen, Tien-Hua and Huang, Ben-Shian and Chen, Po-Han and Su, Cheng-Hsi and Shyu, Jia-Fwu and Tsai, Pei-Jiun
Journal: World Journal of Stem Cells (2020): 139

Participation of extracellular vesicles from zika-virus-infected mosquito cells in the modification of na{\"\i}ve cells’ behavior by mediating cell-to-cell transmission of viral elements
Authors: Mart{\'\i}nez-Rojas, Pedro Pablo and Quiroz-Garc{\'\i}a, Elizabeth and Monroy-Mart{\'\i}nez, Ver{\'o}nica and Agredano-Moreno, Lourdes Teresa and Jim{\'e}nez-Garc{\'\i}a, Luis Felipe and Ruiz-Ordaz, Blanca H
Journal: Cells (2020): 123

Dose-Dependent Effect of Hyperbaric Oxygen Treatment on Burn-Induced Neuropathic Pain in Rats
Authors: Wu, Zong-Sheng and Wu, Sheng-Hua and Lee, Su-Shin and Lin, Cen-Hung and Chang, Chih-Hau and Lo, Jing-Jou and Chai, Chee-Yin and Wu, Ching-Shuang and Huang, Shu-Hung
Journal: International journal of molecular sciences (2019): 1951

Dose-dependent effect of hyperbaric oxygen treatment on burn-induced neuropathic pain in rats
Authors: Wu, Zong-Sheng and Wu, Sheng-Hua and Lee, Su-Shin and Lin, Cen-Hung and Chang, Chih-Hau and Lo, Jing-Jou and Chai, Chee-Yin and Wu, Ching-Shuang and Huang, Shu-Hung
Journal: International journal of molecular sciences (2019): 1951

References

View all 18 references: Citation Explorer

On the mechanism of Trolox as antiblinking and antibleaching reagent
Authors: Cordes T, Vogelsang J, Tinnefeld P.
Journal: J Am Chem Soc (2009): 5018

Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification
Authors: Gallardo-Escarate C, Alvarez-Borrego J, Von Br and E, Dupre E, Del Rio-Portilla MA.
Journal: Biol Res (2007): 29

Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy
Authors: Widengren J, Chmyrov A, Eggeling C, Lofdahl PA, Seidel CA.
Journal: J Phys Chem A (2007): 429

The reliability of long-term storage of direct immunofluorescent staining slides at room temperature
Authors: Dikicioglu E, Meteoglu I, Okyay P, Culhaci N, Kacar F.
Journal: J Cutan Pathol (2003): 430

Comparative genomic hybridization technique
Authors: El-Rifai WE, Knuutila S.
Journal: Methods Mol Med (2001): 25

Fast-in situ hybridization and immunoenzymatic color pigment detection of mouse bone marrow micronucleus
Authors: Kolanko CJ, Pyle MD, Loats H, Parton J, Blakely WF, Nath J.
Journal: Biotech Histochem (1999): 111

Comparison of fixation protocols for adherent cultured cells applied to a GFP fusion protein of the epidermal growth factor receptor
Authors: Brock R, Hamelers IH, Jovin TM.
Journal: Cytometry (1999): 353

Antifading agents for confocal fluorescence microscopy
Authors: Berrios M, Conlon KA, Colflesh DE.
Journal: Methods Enzymol (1999): 55

Evaluation of five green fluorescence-emitting streptavidin-conjugated fluorochromes for use in immunofluorescence microscopy
Authors: Benchaib M, Delorme R, Pluvinage M, Bryon PA, Souchier C.
Journal: Histochem Cell Biol (1996): 253

Immunofluorescent artifacts due to the pH of antifading mounting media
Authors: Swartz DJ, Santi PA.
Journal: Biotechniques (1996): 398