FluoroQuest™ Mounting Medium with DAPI

FluoroQuest™ with DAPI is an aqueous mounting medium for preserving fluorescence of tissue and cell smears. This mounting medium is fortified with DAPI which is a counterstain for DNA. For use in in situ hybridization techniques or other methods where fluorescence of DNA staining is required. DAPI excites at 360 nm and emits at 460 nm, producing a blue fluorescence. RNA is also stained with DAPI.

HeLa cells were stained with mouse anti-tubulin and then followed with iFluor<sup>TM</sup> 610 goat anti-mouse IgG (H+L). Cells were mounted in FluoroQuest™ Mounting Medium with DAPI (Cat# 20004).

Protocol

SDS

COA

Catalog	Size	Price	Quantity
20004	50 mL	Price

Citations

View all 10 citations: Citation Explorer

Hippo signaling pathway regulates Ebola virus transcription and egress

Authors:

Liang, Jingjing and Djurkovic, Marija A and Leavitt, Carson G and Shtanko, Olena and Harty, Ronald N

Journal:

Nature Communications (2024): 6953

Zika Virus-Infected Monocyte Exosomes Mediate Cell-to-Cell Viral Transmission

Authors:

Mart{\'\i}nez-Rojas, Pedro Pablo and Monroy-Mart{\'\i}nez, Ver{\'o}nica and Agredano-Moreno, Lourdes Teresa and Jim{\'e}nez-Garc{\'\i}a, Luis Felipe and Ruiz-Ordaz, Blanca H

Journal:

Cells (2024): 144

Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm

Authors:

Kouadio, Jean-Louis and Zheng, Meiying and Aikins, Michael and Duda, David and Duff, Stephen and Chen, Danqi and Zhang, Jun and Milligan, Jason and Taylor, Christina and Mamanella, Patricia and others,

Journal:

PloS one (2021): e0260532

Structural and functional characterization of Mpp75Aa1. 1, a putative beta-pore forming protein from Brevibacillus laterosporus active against the western corn rootworm

Authors:

Kouadio, Jean-Louis and Duff, Stephen and Aikins, Michael and Zheng, Meiying and Rydel, Timothy and Chen, Danqi and Bretsnyder, Eric and Xia, Chunsheng and Zhang, Jun and Milligan, Jason and others,

Journal:

PloS one (2021): e0258052

Acute melatonin administration improves exercise tolerance and the metabolic recovery after exhaustive effort

Authors:

Faria, Vin{\'\i}cius Silva and Pejon, Taciane Maria Melges and Gobatto, Claudio Alexandre and de Araujo, Gustavo Gomes and Cornachione, Anabelle Silva and Beck, Wladimir Rafael

Journal:

Scientific Reports (2021): 1--11

References

View all 18 references: Citation Explorer

On the mechanism of Trolox as antiblinking and antibleaching reagent

Authors:

Cordes T, Vogelsang J, Tinnefeld P.

Journal:

J Am Chem Soc (2009): 5018

Relationship between DAPI-fluorescence fading and nuclear DNA content: An alternative method to DNA quantification

Authors:

Gallardo-Escarate C, Alvarez-Borrego J, Von Br and E, Dupre E, Del Rio-Portilla MA.

Journal:

Biol Res (2007): 29

Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy

Authors:

Widengren J, Chmyrov A, Eggeling C, Lofdahl PA, Seidel CA.

Journal:

J Phys Chem A (2007): 429

The reliability of long-term storage of direct immunofluorescent staining slides at room temperature

Authors:

Dikicioglu E, Meteoglu I, Okyay P, Culhaci N, Kacar F.

Journal:

J Cutan Pathol (2003): 430

Comparative genomic hybridization technique

Authors:

El-Rifai WE, Knuutila S.

Journal:

Methods Mol Med (2001): 25

Physical properties

Solvent

Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	27000
Excitation (nm)	359
Emission (nm)	457

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Refrigerated (2-8 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on September 26, 2025