FMOC-Asp(biotinyl-PEG)-OH

This PEG biotin-labeled amino acid has excellent solubility in DMF and other solvents used in SPPS. It is an excellent alternative to FMOC-Lys(biotin)-OH (#5020) due to its improved solubility in both organic solvents and water. In addition, the PEG-spacer reduce the hindrance between the target peptide and avidin, leading to better biotin binding. The hydrophilic nature of the PEG minimizes non-specific interactions that can arise from the spacer group becoming buried in the hydrophobic pocket of proteins.

Protocol

COA

Catalog	Size	Price	Quantity
5023	100 mg	Price
5024	1 g	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Asp(biotinyl-PEG)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	127.561 µL	637.804 µL	1.276 mL	6.378 mL	12.756 mL
5 mM	25.512 µL	127.561 µL	255.122 µL	1.276 mL	2.551 mL
10 mM	12.756 µL	63.78 µL	127.561 µL	637.804 µL	1.276 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	783.94 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7

Authors:

Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi

Journal:

Molecules (2014): 2481--2496

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions

Authors:

Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L

Journal:

Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer

Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach

Authors:

Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.

Journal:

Biochemistry (2009): 5753

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization

Authors:

Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.

Journal:

Nucleic Acids Res (2009): e116

Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds

Authors:

Nettels D, Hoffmann A, Schuler B.

Journal:

J Phys Chem B (2008): 6137

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection

Authors:

Bruno JG, Carrillo MP, Phillips T.

Journal:

J Biomol Tech (2008): 109

Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil

Authors:

Oquare BY, Taylor JS.

Journal:

Bioconjug Chem (2008): 2196

Physical properties

Molecular weight	783.94
Solvent	DMF

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet (Catalog 5023)
Safety Data Sheet (Catalog 5024)
Certificate of Analysis

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Page updated on October 22, 2025