FMOC-Asp(DABCYL)-OH

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Physical properties

Molecular weight	648.71
Solvent	DMF

Spectral properties

Absorbance (nm)	454
Correction Factor (280 nm)	0.516

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

648.71

Absorbance (nm)

454

Correction Factor (280 nm)

0.516

A building block for in-sequence Asp labeling by DABCYL.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of FMOC-Asp(DABCYL)-OH to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	154.152 µL	770.76 µL	1.542 mL	7.708 mL	15.415 mL
5 mM	30.83 µL	154.152 µL	308.304 µL	1.542 mL	3.083 mL
10 mM	15.415 µL	77.076 µL	154.152 µL	770.76 µL	1.542 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Absorbance (nm)	454
Correction Factor (280 nm)	0.516

Product Family

Name	Extinction coefficient (cm ^-1 M ^-1)	Correction Factor (280 nm)
FMOC-Asp(EDANS)-OH CAS 182253-73-2	5900	0.107
FMOC-Asp(TF3)-OH	75000¹	0.179
FMOC-Lys(DABCYL)-OH CAS 146998-27-8	-	0.516
FMOC-Asp(TQ2)-OH	21000	0.12
FMOC-Asp(TQ3)-OH	22000	0.091
FMOC-Glu(DABCYL)-OH	-	0.516

Images

Figure 1. Chemical structure for FMOC-Asp(DABCYL)-OH

Citations

View all 2 citations: Citation Explorer

Pharmacophore Generation from a Drug-like Core Molecule Surrounded by a Library Peptide via the 10BASEd-T on Bacteriophage T7
Authors: Tokunaga, Yuuki and Azetsu, Yuuki and Fukunaga, Keisuke and Hatanaka, Takaaki and Ito, Yuji and Taki, Masumi
Journal: Molecules (2014): 2481--2496

Site-specific C-terminal and internal loop labeling of proteins using sortase-mediated reactions
Authors: Guimaraes, Carla P and Witte, Martin D and Theile, Christopher S and Bozkurt, Gunes and Kundrat, Lenka and Blom, Annet EM and Ploegh, Hidde L
Journal: Nature protocols (2013): 1787--1799

References

View all 16 references: Citation Explorer

Profiling the substrate specificity of viral protease VP4 by a FRET-based peptide library approach
Authors: Ekici OD, Zhu J, Wah Chung IY, Paetzel M, Dalbey RE, Pei D.
Journal: Biochemistry (2009): 5753

A FRET-based assay for characterization of alternative splicing events using peptide nucleic acid fluorescence in situ hybridization
Authors: Blanco AM, Rausell L, Aguado B, Perez-Alonso M, Artero R.
Journal: Nucleic Acids Res (2009): e116

Unfolded protein and peptide dynamics investigated with single-molecule FRET and correlation spectroscopy from picoseconds to seconds
Authors: Nettels D, Hoffmann A, Schuler B.
Journal: J Phys Chem B (2008): 6137

Development of DNA aptamers to a foot-and-mouth disease peptide for competitive FRET-based detection
Authors: Bruno JG, Carrillo MP, Phillips T.
Journal: J Biomol Tech (2008): 109

Synthesis of peptide nucleic acid FRET probes via an orthogonally protected building block for post-synthetic labeling of peptide nucleic acids at the 5-position of uracil
Authors: Oquare BY, Taylor JS.
Journal: Bioconjug Chem (2008): 2196

A 'turn-on' FRET peptide sensor based on the mercury binding protein MerP
Authors: White BR, Liljestr and HM, Holcombe JA.
Journal: Analyst (2008): 65

Detecting the inter-peptide arrangement and maturation process of transthyretin (105-115) amyloid fibril using a FRET pair with short Forster distance
Authors: Deng W, Cao A, Lai L.
Journal: Biochem Biophys Res Commun (2007): 689

Enzymatic nanolithography of FRET peptide layer using V8 protease-immobilized AFM probe
Authors: Nakamura C, Miyamoto C, Obataya I, Takeda S, Yabuta M, Miyake J.
Journal: Biosens Bioelectron (2007): 2308

Controlled peptide solvation in portion-mixing libraries of FRET peptides: improved specificity determination for Dengue 2 virus NS2B-NS3 protease and human cathepsin S
Authors: Alves FM, Hirata IY, Gouvea IE, Alves MF, Meldal M, Bromme D, Juliano L, Juliano MA.
Journal: J Comb Chem (2007): 627

Peptide arrays with designed alpha-helical structures for characterization of proteins from FRET fingerprint patterns
Authors: Usui K, Takahashi M, Nokihara K, Mihara H.
Journal: Mol Divers (2004): 209